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30 results about "Donor derived" patented technology

Universal donor-derived tolerogenic cells for inducing non-syngeneic transplantation tolerance

InactiveUS20090232774A1Reduce and preventReduce and prevent graft rejectionOrganic active ingredientsBiocideDiseaseSubject matter
The present invention provides a method of treating a disease in a subject in need thereof via non-syngeneic graft administration without or with reduced concomitant graft rejection. The method comprises administering to the subject a therapeutically effective graft being non-syngeneic with the subject, and a dose of tolerogenic cells being non-syngeneic with both the subject and the graft for preventing or reducing graft rejection in the subject, thereby treating the disease in the subject
Owner:YEDA RES & DEV CO LTD

Method for establishing PDX (Patient Derived Xenograft) model of human blood tumor

The invention discloses a method for establishing a PDX (Patient Derived Xenograft) model of human blood tumor. The method comprises the steps of extracting a blood tumor cell of a patient, adding rabbit anti-human thymocyte immunoglobulin ATG (Anti-Thymocyte Globulin) and patient autologous serum, mixing, incubating and after completing the incubation, re-suspending the obtained cell and inoculating in a mouse; and feeding the inoculated mouse with CsA (Cyclosporine A) and lasting for 5-10 days starting from 2 days before the inoculation of the blood tumor cell. The method disclosed by the invention has the beneficial effects that a novel highly immunodeficient NCG mouse independently developed in China is firstly adopted to establish a human leukemia PDX model; by orally taking human thymocyte immunoglobulin pretreatment sample combined with the cyclosporine for a long term, donor-derived T cells are removed and inhibited; and by combining the toxic effect of the ATG on lymphocytes with the functional blocking effect of the CsA on T lymphocytes, the immune function of the T lymphocytes can be persistently inhibited and the success rate of PDX modeling of the blood tumor is significantly improved.
Owner:JIANGSU PROVINCIAL HOSPITAL OF TCM

Method for determining differential SNP between donor and receptor, and applications thereof

The present invention discloses a method for determining the differential SNP between a donor and a receptor. The method comprises: obtaining first sequencing data and second sequencing data; respectively comparing the first sequencing data and the second sequencing data to a reference sequence to obtain a first comparing result and a second comparing result; respectively based on the first comparing result and the second comparing result, carrying out SNP detection to obtain a first typing results and a second typing result; and comparing the first typing result to the second typing results to determine the differential SNP. The invention discloses a method for determining the proportion of the donor-derived cfDNA in a receptor, and a method and an apparatus for monitoring organ transplant rejection. With the method and / or apparatus of the present invention, the differential SNP between the donor and the receptor can be determined, and the proportion of the donor-derived cfDNA in the receptor can be accurately determined. The method and / or the apparatus of the present invention can be adopted as the assisted or supplementing way for the organ transplant rejection monitoring.
Owner:BGI GUANGZHOU MEDICAL LAB CO LTD +1

Use of nanoparticles coated with red blood cell membranes to enable blood transfusion

The present invention relates to methods, combinations and pharmaceutical compositions for supplying a blood source from a donor source with a mis-matched blood type, or potentially a mis-matched blood type, to a recipient, using, inter alia, an effective amount of a nanoparticle comprising a) an inner core comprising a non-cellular material, and b) an outer surface comprising a cellular membrane derived from a red blood cell, the cellular membrane of the nanoparticle comprising a blood type antigen that exists on the red blood cell from the donor source, but is missing or potentially missing on red blood cells of the recipient.
Owner:CELLICS THERAPEUTICS INC

Testicle cryopreservation agent for marine fishes and preparation method of spermatogonium

The invention provides a testicle cryopreservation agent for marine fishes and a preparation method of spermatogonium. The preparation method of spermatogonium comprises the following main steps: preparing a cryopreservation agent formula suitable for sciaenops ocellatus; establishing a testicle cryopreservation and unfreezing method; preparing the transplantable spermatogonium. The invention establishes a cryopreservation method for the spermatogonium of the sciaenops ocellatus and technical guarantees are provided for a reproductive cell transplanting technology. According to the testicle cryopreservation agent for the marine fishes and the preparation method of the spermatogonium, a donor source for transplanting reproductive cells is expanded; a testicle is cryopreserved for a long period and can be used according to requirements; guarantees are provided for the donor source for transplanting the reproductive cells and an application range of transplanting of the reproductive cells of fishes is greatly widened.
Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof

InactiveCN106047891AEnhanced drought tolerance during reproductive periodIncrease genetic diversityMicrobiological testing/measurementPlant peptidesAgricultural scienceRandom population
The invention relates to a gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and a molecular marker method thereof, belonging to the fields of rice high-yield stress-tolerant breeding and molecular genetics. By utilizing Jijing 88 and 3 donor-derived breeding population selection, a novel segregation distortion locating process suitable for breeding population selection is adopted to locate drought-tolerance-related QTL (quantitative trait loci); and random population selection is utilized to verify the authenticity of the drought-tolerant QTL and the reliability of the close linkage marker. When being applied to auxiliary selective breeding and polymerization breeding of drought-tolerant rice, the gene can effectively overcome the defects in the past spontaneous mutant genes, can obviously lower the negative influence on the auxiliary breeding due to the development of the initial locating result, and can carry out genotype selection on the lower-generation breeding populations in the seedling stage to obtain the drought-tolerance individuals, thereby facilitating the timely hybrid transformation, omitting the drought resistance identification process in the adult-plant stage, enhancing the breeding efficiency and accelerating the breeding progress.
Owner:AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI +1

Compositions and multiplex assays for characterizing active proteases and their inhibitors

Proteases regulate a wide range of normal cellular functions where dysregulated activity is observed in various diseases. Compositions and methods use protease activity multiplexed bead-based immunoassays to profile protease activity. This platform technology integrates protease activity measurements with total protein quantification techniques. It represents a significant improvement over existing detection techniques by allowing for multiplexed, sensitive active protease measurements in complex biological samples. Exemplary multiplexed detections are realized in a single assay using a minute sample amount (e.g., 5 μl) for active recombinant MMP-1, -2, -3, -7, 9, and 12 and those same MMPs in cell culture supernatant, menstrual fluid effluent, and peritoneal aspirates. This multiplexed platform achieves high level of sensitivities equal to or better than existing leading single-plex detection strategies. It also allows for high throughput screening to identify inhibitors of proteases in complex, donor-derived samples.
Owner:MASSACHUSETTS INST OF TECH

A non-invasive method for monitoring transplanted organ status in organ-transplant recipients

The present invention pertains to a non-invasive method for monitoring transplanted organ status in organ-transplant recipients by determining the ratio of donor derived marker sequences to the marker sequences of the transplant recipient from circulating cell free DNA of the transplant recipients using digital droplet PCR. The invention also determined a normalized threshold value of the total circulating cell free nucleic acids healthy as well as in post-transplantation patients for assessing and monitoring transplanted organ status in organ-transplant recipients.
Owner:ACRANNOLIFE GENOMICS PVT LTD

Method for predicting organ transplant rejection using next-generation sequencing

A non-invasive method for organ transplant rejection prediction is described, involving measurement of the ratio between donor-specific nucleic acid sequences and recipient-specific nucleic acid sequences in a biological sample obtained from an organ transplant recipient. In specific implementations, the method includes analyzing a biological sample (e.g., blood) obtained from an organ transplant recipient to measure the ratio between donor-derived marker sequences and recipient-derived marker sequences, having three or more markers selected from the markers listed in Tables 1 to 10, and thereby predicting organ transplant rejection based on the ratio. Using next-generation sequencing (NGS) or digital base amplification in the disclosed method enables its application to minute amounts of a sample. The method is rapid, inexpensive, enables rapid data analysis, is applicable irrespective of organ type and race(s) of the donor and recipient, and can detect the probability of sequencing error.
Owner:EONE DIAGNOMICS GENOME CENT

Improved liquid biopsy using size selection

Provided herein are improved methods of determining the sequences of cell-free DNA (cfDNA). The methods in certain embodiments are used for the analysis of circulating DNA in serum samples, such as circulating fetal DNA, circulating donor derived DNA, or circulating tumor DNA. In certain embodiments, the methods include selectively enriching trinucleosomal, dinucleosomal, mononucleosomal or sub-mononucleosomal DNA from the isolated cfDNA.
Owner:NATERA

Construction method for hepatic progenitor cell-like cell bank and cell lines prepared by method and applications of cell bank and cell lines

The invention provides a construction method for a hepatic progenitor cell-like cell bank. The method includes the following steps: performing transformation culture on human primary hepatocyte cultures derived from different donors, performing cryopreservation treatment, performing propagation culture, performing primary passage treatment, performing virus infection, performing secondary passagetreatment, performing continuous screening culture and performing continuous passage culture. According to the construction method for the heterogeneous immortal hepatic progenitor cell-like cell bankprovided by the invention, the human primary hepatocyte cultures derived from the donors are firstly subjected to transformation culture before proliferation culture, so that the method is beneficialto giving good proliferation performance to the human primary hepatocyte cultures, and culture parameters are controlled in the subsequent stage, so that obtained immortal hepatic progenitor cell-like cell lines derived from the different donors have good in-vitro proliferation ability. The invention also provides an application of the liver progenitor cell-like cell bank and the cell lines obtained by the construction method.
Owner:SHANGHAI CELLIVER BIOTECHNOLOGY CO LTD

Skin graft used for xenogeneic intermingled transplantation

The invention provides a skin graft used for xenogeneic intermingled transplantation. The skin graft comprises a pigskin graft and an autologous skin graft, and is prepared by adopting the following method: (1) preparation of the pigskin graft; and (2) preparation of the autologous skin graft. According to the invention, a pig serves as a donor, and the donor is wide in source and is processed into a special shape, so a wound surface can be conveniently repaired by embedding and intermingled transplantation of the donor with the autologous skin of a patient in a later period; the current situation of skin source shortage for the patient with large-area skin defects like burns and skin avulsion can be greatly relieved; the injury of a donor site is reduced; the life of the patient is saved;great social value and large industrialization and commercialization values are achieved; and considerable economic values are brought after the skin graft is put into the clinical market.
Owner:陈翔

A human chondroblastoma cell line and its application

ActiveCN111662875BStable in situ tumorigenic abilityWith gene modification functionMicrobiological testing/measurementDrug screeningGenes mutationBlastoma
The present invention relates to a human chondroblastoma cell line and its application. The human chondroblastoma cell line has a deposit number of CCTCC NO: C2020110, and its donor source is a clinical histone H3.3K36M mutant chondroblastoma patient, and the 36th lysine on H3F3B is mutated to methionine Mutation of acid gene, the three-lineage differentiation of osteogenic, chondrogenic and adipogenic is blocked. It has the ability to form tumors in situ of the tibia and has stable tumorigenic ability. It can be transfected by lentivirus and has the function of gene modification. The human chondroblastoma cell line can be used for screening of drugs for the treatment of chondroblastoma, construction of a drug-resistant chondroblastoma model, construction of an animal model of chondroblastoma, screening of chondroblastoma-related biomarkers and research Mechanisms of the development of chondroblastoma. The invention provides experimental materials for researchers to deeply develop the occurrence and development mechanism of human chondroblastoma and to find or evaluate treatment plans.
Owner:SHANGHAI FIRST PEOPLES HOSPITAL

Gene QDTY11.5<IR66897B> for substantially enhancing drought tolerance of rice in reproductive stage, and molecular marking method thereof

InactiveCN106434687ARich diversityEnhanced drought tolerance during reproductive periodMicrobiological testing/measurementPlant peptidesAgricultural scienceGenotype
The invention relates to a gene QDTY11.5<IR66897B> for substantially enhancing the drought tolerance of rice in a reproductive stage, and a molecular marking method thereof, and belongs to the fields of rice high-yield and anti-adversity breeding and molecular genetics. QDTY11.5 is identified from Jijing88 and four donor derived breeding selection colonies through a segregation distortion positioning technology, is positioned at the 77.8 cM position of eleventh chromosome, is verified from three random selection colonies through a single-labeling analysis technology, and is closely linked and labeled to an economical molecular marker RM229. The gene is suitable for rice drought-resistant molecule assisted selective breeding and polymerization breeding, effectively covers the shortages of previous natural variation genes, allows drought-resistant individuals to be obtained through genotype selection of low-generation breeding colonies in the seedling stage, so hybridization transfer breeding is timely to carry out, the drought resistance identification process in the adult plant stage is omitted, the breeding efficiency is improved, and the breeding process is accelerated.
Owner:AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI +1

Optimizing Detection of Transplant Injury by Donor-Derived Cell-Free DNA

PendingUS20220073989A1Rapid and inexpensive and effectiveMicrobiological testing/measurementCell freeCancer research
Herein are novel methods of detecting subacute and active rejection in graft recipients, including kidney recipients by the measurement of donor-derived cell-free DNA. By the methods, active rejection processes encompassing T-cell mediated rejection may be detected. Also provided herein are novel threshold values for the determination of active rejection that enable higher sensitivity and specificity than prior methods. Additionally, by donor-derived cell-free DNA, subacute rejection processes can be detected, including borderline rejection and other graft injuries.
Owner:RGT UNIV OF CALIFORNIA

Compositions for the treatment of skin conditions

Described herein are methods and compositions for the treatment of skin conditions associated with dysbiosis. Skin conditions associated with dysbiosis for treatment using compositions and methods described herein include atopic dermatitis, eczema, dermatitis, psoriasis, rosacea, and acne. Compositions include single or more than one strain of healthy donor derived bacteria for administration to provide therapy for skin conditions associated with dysregulated microbiota. Such compositions include gram negative and / or gram positive bacteria.
Owner:FORTE SUBSIDIARY INC

Methods for detection of donor-derived cell-free DNA

The present disclosure provides methods for determining the status of an allograft within a transplant recipient from genotypic data measured from a mixed sample of DNA comprising DNA from both the transplant recipient and from the donor. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.
Owner:NATERA

New blood vessel construct implanted into subcutaneous tissue of subject and preparation method thereof

The invention discloses a new blood vessel construct implanted into the subcutaneous tissue of a subject and a preparation method thereof, belonging to the technical field of high polymer materials. The new blood vessel construct is a nanofiber catheter prepared from a high polymer material in an electrostatic spinning manner, wherein the material is one selected from the group consisting of poly-L-lactic acid, poly-D-lactic acid, a polylactic acid-glycolic acid copolymer and polyglycolic acid. When the blood vessel construct prepared from the high polymer material is transplanted into subcutaneous tissue, an extracellular matrix space rich in new blood vessels can be rapidly formed under the skin of a subject and used for containing a donor-derived pancreatic island or pancreatic island cells to maintain the functions thereof for a long time.
Owner:GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER

Multi-batch primary separation method of same-donor-derived human mesenchymal stem cells

The invention discloses a multi-batch primary separation method of same-donor-derived human mesenchymal stem cells, and belongs to the field of cell culture. According to the method, a donor tissue istreated to obtain 1 to 5 mm <3> donor tissue small blocks, and the donor tissue small blocks are soaked with fetal bovine serum or human AB serum for processing, and then subjected to conventional tissue block adherent culture. While harvesting of the mesenchymal stem cells, the donor tissue small blocks are transferred to a new culture plate for continuously culturing, and the culture supernatant of the tissue blocks is recovered and continuously incubated to achieve high-quality, multi-batch stable separation of the same-donor-derived human mesenchymal stem cells. The morphology, proliferation and differentiation ability and immunophenotype of the cells obtained by method remain unchanged among batches. Compared with a traditional tissue block adherence method and an enzymatic digestionmethod, the method avoids the waste of clinical specimen resources, saves human and material resources of clinical links, and improves the separation efficiency and yield of the mesenchymal stem cells.
Owner:GUANGDONG PHARMA UNIV

Compositions and multiplex assays for characterizing active proteases and their inhibitors

Proteases regulate a wide range of normal cellular functions where dysregulated activity is observed in various diseases. Compositions and methods use protease activity multiplexed bead-based immunoassays to profile protease activity. This platform technology integrates protease activity measurements with total protein quantification techniques. It represents a significant improvement over existing detection techniques by allowing for multiplexed, sensitive active protease measurements in complex biological samples. Exemplary multiplexed detections are realized in a single assay using a minute sample amount (e.g., 5 μl) for active recombinant MMP-1, -2, -3, -7, 9, and 12 and those same MMPs in cell culture supernatant, menstrual fluid effluent, and peritoneal aspirates. This multiplexed platform achieves high level of sensitivities equal to or better than existing leading single-plex detection strategies. It also allows for high throughput screening to identify inhibitors of proteases in complex, donor-derived samples.
Owner:MASSACHUSETTS INST OF TECH

Method and kit for detecting presence or proportion of donors in acceptor sample

The invention relates to a method for detecting SNP sites of a donor-sourced sample and a receptor-sourced sample. Further, the present application also relates to a method for detecting the presence or proportion of donors in an acceptor sample, and a kit for implementing the method.
Owner:XIAMEN UNIV

A multi-batch primary isolation method of human mesenchymal stem cells from the same donor source

The invention discloses a multi-batch primary separation method of human mesenchymal stem cells from the same donor, belonging to the field of cell culture. The method of the present invention is to obtain a 1‑5mm tissue after the donor tissue is processed. 3 The small pieces of donor tissue were treated by soaking in fetal bovine serum or human AB serum, and then carried out conventional tissue block adherent culture. While harvesting mesenchymal stem cells, the donor tissue block is continuously cultured on a transfer plate, and the culture supernatant of the tissue block is recovered to continue incubation to achieve high-quality, multi-batch stable isolation of human mesenchymal stem cells from the same donor . The morphology, proliferation and differentiation ability and immunophenotype of the cells obtained in the present invention remain unchanged among batches. Compared with the traditional tissue block attachment method and enzyme digestion method, the method of the present invention avoids the waste of clinical specimen resources, saves manpower and material resources in clinical links, and also improves the separation efficiency and yield of mesenchymal stem cells.
Owner:GUANGDONG PHARMA UNIV

Detection method for evaluating transplantation injury and rejection risk after liver transplantation

The invention discloses a detection method for evaluating transplantation injury and rejection risks after liver transplantation, which comprises the following steps: 1) respectively adding primers and probes by taking genome DNA (Deoxyribose Nucleic Acid) of a donor source and genome DNA of a receptor source as templates; 2) performing qPCR detection on the DNA region where the SNP site is located; (3) respectively screening out 1 to 2 receptor homozygous SNP sites and donor homozygous SNP sites; 4) extracting free DNA of the receptor sample as a template, selecting a primer and a probe corresponding to the selected SNP site, adding the primer and the probe into the free DNA, and then carrying out ddPCR detection; and 5) analyzing the ddPCR result, and calculating the percentage of the free DNA containing the SNP site in the donor in the free DNA of the receptor sample. According to the detection method for evaluating the injury and rejection risk of the graft after the liver transplantation, the injury condition and rejection risk of the liver graft can be sensitively, specifically and accurately evaluated, any wound detection is not needed, the operation is convenient, the time is saved, and the efficiency is improved.
Owner:WUXI DIAGNOSTICS LAB SHANGHAI CO LTD
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