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Method For Predicting Organ Transplant Rejection Using Next-Generation Sequencing

A next-generation sequencing and organ technology, applied in the direction of biochemical equipment and methods, instruments, measuring devices, etc., can solve a lot of time and cost problems

Pending Publication Date: 2018-02-02
EONEDIAGNOMICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, this method has limitations due to the time and cost it requires to analyze genome-wide data

Method used

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  • Method For Predicting Organ Transplant Rejection Using Next-Generation Sequencing
  • Method For Predicting Organ Transplant Rejection Using Next-Generation Sequencing
  • Method For Predicting Organ Transplant Rejection Using Next-Generation Sequencing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Example 1: Predicting Organ Transplant Rejection in Artificially Generated Organ Transplant Recipients

[0126] 1.1: Pretreatment for preparation and analysis of artificial DNA samples from organ transplant recipients

[0127] Mixing male DNA (donor) with female DNA (recipient) so that the percentage of male DNA in female DNA is 0%, 0.625%, 1.25%, 2.5%, 5% or 10%, thereby preparing artificial organ transplant patients Genomic DNA samples.

[0128] Custom amplicons were prepared for the TruSeq Custom Amplicon (TSCA) assay (Illumina, USA) using 100 ng of each gDNA. The heat block was adjusted to 95°C, and 5 μl each of DNA and CAT (Custom Amplicon OligoTube) was added to a 1.7-ml test tube. 5 µl each of ACD1 and ACP1 were also prepared as control reagents. Add 40 μl of OHS1 (Oligonucleotide Hybridization Reagent for Sequencing 1) to each tube, mix well using a pipette, maintain each tube at 95°C for 1 minute, then pass it over the oligonucleotide at 40°C Hybridizat...

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Abstract

The present invention relates to a method for non-invasively predicting an organ transplant rejection by measuring the ratio between an organ-donor-specific nucleic acid sequence and a recipient-specific nucleic acid sequence in a biological sample of an organ transplant recipient and, more specifically, to a method for predicting an organ transplant rejection by measuring the ratio of a donor-derived marker sequence and a recipient-derived marker sequence regarding three or more markers selected in Tables 1 to 10 by testing a biological sample (e.g. blood) of an organ transplant recipient andpredicting the organ transplant rejection based on the measurement. The method for predicting an organ transplant rejection using next-generation sequencing (NGS) according to the present invention or a digital base amplification technique can apply even to a very small amount of sample, is faster and inexpensive, is fast in the rate of data analysis, can apply to all organ transplants of all races in the word, and can simultaneously detect error probability of a sequence analysis, and thus is useful to non-invasively predict the organ transplant rejection.

Description

technical field [0001] The present application relates to a method for non-invasively predicting organ transplant rejection by determining the ratio between donor-specific nucleic acid sequences and recipient-specific nucleic acid sequences in biological samples obtained from organ transplant recipients, and more specifically, to A method of predicting organ transplant rejection based on the results of determining the ratio between a donor-derived marker sequence and a recipient-derived marker sequence by analyzing a biological sample (such as blood) obtained from an organ transplant recipient. Background technique [0002] Accurate and timely diagnosis of organ transplant rejection in organ transplant recipients is essential for the survival of organ transplant recipients. However, currently used methods for diagnosing organ transplant rejection have many drawbacks. For example, the gold standard for diagnosing heart transplant rejection is the use of cardiac biopsy proced...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G06F19/10G06F19/22G16B30/00
CPCG16B99/00G16B30/00C12Q1/6827C12Q1/6869C12Q1/6876C12Q2600/106C12Q2600/16C12Q2600/156C12Q2537/143C12Q1/68G01N33/49C12Q1/6881C12Q1/6883G01N2800/245
Inventor 李旻燮裵真植
Owner EONEDIAGNOMICS CO LTD
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