Gene QDTY11.5<IR66897B> for substantially enhancing drought tolerance of rice in reproductive stage, and molecular marking method thereof
A technology of QDTY11.5IR66897B and IR66897B is applied in the field of significantly increasing the drought tolerance gene QDTY11.5IR66897B and its molecular markers during the reproductive stage of rice, which can solve the problems of lack of large-effect genes in molecular assisted breeding, difficult successful application of genes, and insufficient connection. Achieve the effects of facilitating hybridization, enriching diversity, and speeding up the breeding process
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[0017] (1) Mapping of Drought Tolerance QTL
[0018] 1. Test materials
[0019] Firstly, three landrace varieties IR66897B (male parent of P1 population), MR77 (male parent of P2 population) and SN265 (male parent of P3 population) screened from the germplasm resource bank were constructed respectively with Jijing 88, a high-yielding, high-quality, drought-tolerant japonica rice in China. 3 high-generation backcross introduction line groups. After two rounds of drought stress selection in the reproductive period, 81 drought-tolerant lines from 3 families were selected to form a selective breeding population (Table 1).
[0020] Table 1. Composition information of 3 selective breeding populations
[0021]
[0022] N 1 is the original BC without drought stress 2 f 2 Group size; N 2 is the coefficient of drought-tolerant plants selected after the first round of drought stress treatment; N 3 is the coefficient of drought-tolerant plants selected after two rounds of drough...
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