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Gene QDTY11.5<IR66897B> for substantially enhancing drought tolerance of rice in reproductive stage, and molecular marking method thereof

A technology of QDTY11.5IR66897B and IR66897B is applied in the field of significantly increasing the drought tolerance gene QDTY11.5IR66897B and its molecular markers during the reproductive stage of rice, which can solve the problems of lack of large-effect genes in molecular assisted breeding, difficult successful application of genes, and insufficient connection. Achieve the effects of facilitating hybridization, enriching diversity, and speeding up the breeding process

Inactive Publication Date: 2017-02-22
AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the mining of these resistance-related genes helps us to understand the genetic basis of drought resistance in rice, most of the genes are still difficult to be successfully applied in breeding practice
On the one hand, there is a gap between basic scientific research and breeding practice research, and the connection is not enough; on the other hand, it is due to the lack of large-effect genes that can be applied to molecular assisted breeding

Method used

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  • Gene QDTY11.5&lt;IR66897B&gt; for substantially enhancing drought tolerance of rice in reproductive stage, and molecular marking method thereof
  • Gene QDTY11.5&lt;IR66897B&gt; for substantially enhancing drought tolerance of rice in reproductive stage, and molecular marking method thereof
  • Gene QDTY11.5&lt;IR66897B&gt; for substantially enhancing drought tolerance of rice in reproductive stage, and molecular marking method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Mapping of Drought Tolerance QTL

[0018] 1. Test materials

[0019] Firstly, three landrace varieties IR66897B (male parent of P1 population), MR77 (male parent of P2 population) and SN265 (male parent of P3 population) screened from the germplasm resource bank were constructed respectively with Jijing 88, a high-yielding, high-quality, drought-tolerant japonica rice in China. 3 high-generation backcross introduction line groups. After two rounds of drought stress selection in the reproductive period, 81 drought-tolerant lines from 3 families were selected to form a selective breeding population (Table 1).

[0020] Table 1. Composition information of 3 selective breeding populations

[0021]

[0022] N 1 is the original BC without drought stress 2 f 2 Group size; N 2 is the coefficient of drought-tolerant plants selected after the first round of drought stress treatment; N 3 is the coefficient of drought-tolerant plants selected after two rounds of drough...

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Abstract

The invention relates to a gene QDTY11.5<IR66897B> for substantially enhancing the drought tolerance of rice in a reproductive stage, and a molecular marking method thereof, and belongs to the fields of rice high-yield and anti-adversity breeding and molecular genetics. QDTY11.5 is identified from Jijing88 and four donor derived breeding selection colonies through a segregation distortion positioning technology, is positioned at the 77.8 cM position of eleventh chromosome, is verified from three random selection colonies through a single-labeling analysis technology, and is closely linked and labeled to an economical molecular marker RM229. The gene is suitable for rice drought-resistant molecule assisted selective breeding and polymerization breeding, effectively covers the shortages of previous natural variation genes, allows drought-resistant individuals to be obtained through genotype selection of low-generation breeding colonies in the seedling stage, so hybridization transfer breeding is timely to carry out, the drought resistance identification process in the adult plant stage is omitted, the breeding efficiency is improved, and the breeding process is accelerated.

Description

technical field [0001] The present invention relates to a kind of gene QDTY 11.5 that significantly increases the drought tolerance gene of rice reproductive stage IR66897B and its molecular marker method, the gene significantly increases the yield of rice under drought stress, belongs to the field of rice high-yield stress-resistant breeding and molecular genetics, and is suitable for introducing the drought-tolerant gene QDTY 11.5 in the reproductive period in rice high-yield stress-resistant breeding IR66897B , and the molecular assisted selection breeding of the gene was carried out by using the closely linked molecular marker RM229. Background technique [0002] Rice is one of the most important food crops in my country and in the world. The annual output of rice accounts for about half of my country's total food production. Rice is the crop that consumes the most water in the world, but my country's fresh water resources are quite scarce and unevenly distributed, maki...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12Q1/68C12N15/11
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐建龙黎志康陈凯朱亚军孟丽君崔彦茹
Owner AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
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