The invention relates to a temperature control method of laser medium of a disk laser and a device thereof. The method comprises the steps that temperature control is performed on laser medium in different regions through a semiconductor refrigerating chip array; firstly reference temperature is set, laser medium subregions, the temperature of which is higher than the reference temperature, are refrigerated, and laser medium subregions, the temperature of which is lower than the reference temperature, are heated; the device comprises the laser medium, a heat sink connected with the laser medium, heat-conducting glue, a temperature sensor on the heat-conducting glue, the semiconductor refrigerating chip array, a closed-loop circuit control system and a computer system; the temperature sensor detects the practical temperature of the laser medium subregions in real time, sends the practical temperature to the computer system, and sends temperature control signals after the practical temperature is compared to the reference temperature; and a closed-loop circuit feeds back the temperature control signals to semiconductor refrigerating chips in real time, so as to realize the real-time control to the laser medium subregions, and to realize the bulk temperature control to the laser medium. The method can dynamically control the temperature of the laser medium to be evenly distributed, and effectively restrain the problem of the thermal effect of the laser medium.