96 results about "Desoxyribonucleotide" patented technology

A process to obtain linear double-stranded covalently closed DNA "dumbbell" constructs from plasmids by restriction digest, subsequent ligation with hairpin oligodesoxyribonucleotides, optionally in the presence of restriction enzyme, and a final digestion with endo- and exonucleolytic enzymes that degrade all contaminating polymeric DNA molecules but the desired construct. The invention also provides a process to obtain said dumbbell constructs employing endonuclease class II enzymes. Furthermore, the invention provides a process to obtain linear, covalently closed DNA molecules, such as plasmids, free from contamination by genomic DNA, by submitting the DNA preparation to a facultative endonucleolytic degradation step and an obligatory exonucleolytic degradation step.

A nucleic acid, which is provided in a large amount through a nucleic acid amplification reaction with the use of chimeric oligonucleotide primers, is constructed in a state of containing a modified deoxyribonucleotide for immobilizing the nucleic acid to a solid phase and then immobilized to a solid phase at a high efficiency, thereby giving an immobilized nucleic acid product with excellent qualities.

Point-of-care binding assays include at least one target nucleic acid binding in a multiplex structure with at least one sequence in a partner nucleic acid associated with a label, due to complementary base pairings between at least one sequence in the target nucleic acid and at least one sequence in the partner nucleic acid. The assays overcome the inherent deficiencies of antibody-protein antigen assays. In a preferred embodiment, color tagged nucleic acid sequences are used to bind a complementary target nucleic acid. The tagged nucleic acid sequences are preferably made from deoxyribonucleotides, ribonucleotides, or peptide nucleotides.

The invention discloses a specific promoter of a root and a recombinant expression vector. The promoter adopts DNA molecular: a of following a) or b) or c) DNA molecular: a) is a DNA molecular which is composed of the deoxyribonucleotide shown in the sequence 1 in the sequence table; b) is a DNA molecular hybridized with a) defined DNA sequence under the strict condition; and c) is a specific expression DNA molecular in the root of the plant and has over 90 percents of homology and the gene with a adjusting and controlling purpose with a) defined DNA sequence. The invention also discloses a recombinant expression vector with the promoter, and the recombinant expression vector can be applied for cultivating the transgenic plant with a specific expressing target gene in the plant root.

A sepsis microarray detection chip includes a plurality of probes immobilized on a matrix, wherein each probe is selected from the group of deoxyribonucleotide sequences depicted in the SEQ ID NOs. 1 to 66. Since these probes are formed with deoxyribonucleotide sequences specific to sepsis, they can be used to detect sepsis.

Methods are provided for evaluating the fidelity of a given set of polymerase chain reaction conditions. In the subject methods, a template polydeoxyribonucleotide is amplified under the to be evaluated polymerase chain reaction conditions, where the template polydeoxyribonucleotide includes a pseudo restriction endonuclease restriction site. The resultant amplified product population is then contacted with the corresponding restriction endonuclease and resultant cleavage products, if any, are detected. The fidelity of the polymerase chain reaction conditions is then derived from the detected cleavage products (or absence thereof). Also provided are kits for use in practicing the subject methods. The subject methods are suited for determining the fidelity of a given polymerase under PCR conditions, and are particularly suited for determining the fidelity of a thermostable polymerase under PCR conditions.

The present invention provides methods and compositions for producing a plurality of labeled deoxyribonucleotides from an initial nucleic acid sample. In the subject methods, a solid support bound polyA+ RNA fraction is first produced from the initial nucleic acid sample. The resultant solid support bound fraction is then contacted with a plurality of gene specific primers, followed by annealed gene specific primer primed synthesis of a plurality of labeled deoxyribonucleotides. Also provided are kits for use in practicing the subject methods.

The invention provides a salmon small molecule polydeoxyribonucleotide (SMPDRN) product with a molecular weight concentrated in an efficacy range of the salmon small molecule polydeoxyribonucleotide product, a controllable precise preparation method of the salmon small molecule polydeoxyribonucleotide product, and application of the salmon small molecule polydeoxyribonucleotide product to the fields of cosmetics, medicine, nutritious food and health food. The molecular weight of small molecule polydeoxyribonucleotide is 50-1000 bp, and the mass of the molecular weight of the small molecule polydeoxyribonucleotide at the 50-500 bp fragment accounts for more than 85% of the total mass of the small molecule polydeoxyribonucleotide; and the molecular weight of the SMPDRN is concentrated in thehigh-efficacy range, the pertinence is higher, the efficacy is more stable, and the obtained product has significant effects in improving cell activity, promoting collagen synthesis, regenerating damaged skin, promoting wound healing, tightening skin, increasing skin elasticity, eliminating wrinkles, delaying skin aging, resisting oxidation, preventing formation of color spots, resisting inflammation, repairing damaged cells and other aspects by being verified by cell, animal and human experiment, and is better than an existing PDRN product.

The invention provides an electrochemical sensor for detecting mercury as well as a preparation method and application of the electrochemical sensor. The electrochemical sensor comprises a glassy carbon electrode, a probe P2 and a mercapto-modified probe P3 which is fixed on gold nano-particles; the surface of the detection end of the glassy carbon electrode is modified with carbon-doped graphite-like carbon nitride; the gold nano-particles deposit on the carbon-doped graphite-like carbon nitride; a mercapto-modified probe P 1 is linked to the gold nano-particles; a part of deoxyribonucleotide of the probe P2 can be in complementary pairing with a part of deoxyribonucleotide of the mercapto-modified probe P1 to form a double-chain structure; and unpaired deoxyribonucleotide in the probe P1 and probe P2 and the mercapto-modified probe P3 which is fixed on the gold nano-particles form the double-chain structure. The preparation method comprises the following steps of modifying the graphite-like carbon nitride, modifying nanogold, modifying the probe P1 and the like. The electrochemical sensor provided by the invention has strong ability of resisting heavy metal ion interference and can be applied to detecting mercury ions.

The invention discloses an SNP label relevant to root characteristics in a wheat seedling stage and application. The invention provides a genotypic substance of 19-site deoxyribonucleotide of AX-110602569-7A gene in a detected wheat genome provided in the invention in application of authentication or auxiliary authentication for wheat root characteristics, and the 39cM position of the AX-110602569-7A gene in wheat chromosome 7AS. The invention provides a rated site QRL.caas-7A for root characteristics in the wheat seedling stage and the SNP site of the site for auxiliary sieving of the rated genes in the root system in the wheat seedling stage. Wheat with excellent related root characteristics can be sieved by using the SNP site, and the SNP plays an important role in cultivating water andfertilizer high-efficiency wheat varieties.

The invention provides a reverse transcription obstruction probe for rapidly removing target RNA in RNA library construction and application thereof. The probe has a length of 20-40 nt, is strictly complementarily paired with a target RNA sequence, and has no complementary pairing continuously exceeding 15 nt with other non-target RNAs, the Tm value of the probe is not lower than 80 DEG C, the self complementary value is less than 5, and 3'-OH of the probe is sealed by modified deoxyribonucleotide. According to the probe, a reverse transcription inhibition probe method is utilized to realize high-efficiency and high-specificity inhibition on reverse transcription of the target RNA during synthesis of one strand of cDNA, so that the target RNA is prevented from participating in a downstream library construction process. The probe has the advantages of simplicity in operation (one-step operation), extremely short consumed time (2 minutes), completeness in retention of the non-target RNAs, high specificity, high efficiency, low cost, high gene detection number and the like, and is very suitable for the fields of industrial automatic library building and rapid disease diagnosis.

The invention discloses a method and a special product for the assisted identification of swine backfat thickness character. The method for the assisted identification of swine backfat thickness character comprises the following steps of: determining whether the deoxyribonucleotide 47033 of the GenBank Accession Number GU565976.1 of a peroxisome proliferator-activated receptor delta gene of a to be detected swine is T, or C, or T and C, so as to determine whether the genotype of the to be detected swine is TT or TC or CC, and determining the backfat thickness character according to the genotype of the to be detected swine, wherein the backfat thickness of the to-be-detected swine with the TT genotype is greater than that of the to be detected swine with the TC genotype, and the backfat thickness of the to be detected swine with the TC genotype is greater than that of the to be detected swine with the CC genotype. The method and the special product disclosed by the invention are used for breeding swine, so that the to be detected swine can be screened in the early stage, the problem of long time needed for selecting good breeding swine in the practical production can be effectively solved, the breeding cost is reduced, and the swine backfat thickness in the practical production is effectively reduced or increased.

The invention discloses a method and a kit for detecting the correlated mutation allele of a total farrow number of a sow as well as the application. The method adopts a PCR (Polymerase Chain Reaction)-SSCP (Single-Strand Conformation Polymorphism) method, PCR augmentation comprises a section from 315-bit deoxyribonucleotide at the 5' terminal of Gen Bank Accession Number AX752829, PCR augmentation products are detected by gel electrophoresis, and the gene type of the sow to be detected is confirmed according to an electrophoresis result: the gene type of the sow to be detected can be GG or AA which means a homozygote if the electrophoresis result presents two straps, and the gene type of the sow to be detected can be GA which means a heterozygote if the electrophoresis result presents three straps. The detection method of the invention has simple operation, low expense and high accuracy, can realize the automatic direct detection and has higher practical breeding application values. The method and the kit of the invention can be applied to breed grices, early select grices to be selected, effectively solve the problem of long time for selecting excellent grices in practical production, decrease the breeding expense, increase the total sow farrow number and improve the economic benefits.

The invention discloses a shuttle plasmid and a derivative plasmid thereof. A nucleotide sequence of the shuttle plasmid which is provided by the invention is a sequence 1 in a sequence table and a 4872-5664th deoxyribonucleotide from 5'end of the sequence 1 in the sequence table is a kanamycin resistance gene and a promoter thereof. The invention also discloses the derivative plasmid thereof, which is obtained through inverting the kanamycin resistance gene of the 4872-5664th deoxyribonucleotide from the 5'end of the sequence 1 in the sequence table and the promoter of the kanamycin resistance gene and through inserting transcription terminators of a reporter gene and a corynebacterium glutamate LeuB gene in multiple cloning sites, the direction of the reporter gene is opposite to the direction of the kanamycin resistance gene and the promoter of the kanamycin resistance gene, and the transcription terminators of the corynebacterium glutamate LeuB gene are located between the reporter gene and the kanamycin resistance gene. The two plasmids have high stability and can obtain wide application on stic engineering.

The invention discloses a method for assisting the judgment of a Wuzhishan minipig inbred line. The method for assisting the judgment of the Wuzhishan minipig inbred line and / or a non-Wuzhishan-mountain minipig inbred line, which is provided by the invention, detects that the 1400th position of deoxyribonucleotide from the 5' end of GenBankAccession Number DQ406743 of a pig to be detected is C or T, and determines that the genotype of the pig to be detected is CC, CT or TT; the pig to be detected with the CC genotype is an alternate Wuzhishan minipig inbred line; and a pig with the CT genotype and a pig to be detected with the TT genotype are non-Wuzhishan-minipig inbred lines. The method can be applied to the seed breeding of the Wuzhishan minipig inbred line. The method can firstly carry out preliminary screening on all the pigs in a swinery to be detected, eliminates the non-Wuzhishan-minipig inbred line, finds out the alternate Wuzhishan minipig inbred line and combines with other methods to carry out further validation. The invention can also be used for detecting whether the Wuzhishan minipig inbred line purchased in the market is counterfeit or not.

There is described the preparation of polymers from nucleotide and / or non-nucleotide monomers, in particular the preparation of natural and / or base-modified and / or sugar-modified and / or phosphate group-modified oligodeoxyribonucleotides and / or oligoribonucleotides and / or non-nucleotide polymers in an enzymatic manner in 3'->5' direction using a RNA ligase.

The invention discloses a kit for single cell genome-wide amplification and application thereof. The invention protects a kit for single cell genome amplification first, comprising a second amplification system; the second amplification system is composed of 10*Phi29buffer, water and a first amplification system; the first single amplification system is composed of dNTP (1.8-2.2)*10-8 mol, N8 primers (1.8-2.2)*10-11 mol in total concentration, BSA (4.5-5.5)*10-3 mg, Pluronic F68 0.0045-0.0055Mul, and DNA polymerase 9-11 U Phi29; the N8 primers are random primers, composed of eight deoxyribonucleotides, in a random arrangement of A, G, C and T. The WGA kit low in cost, high in efficiency and stable in amplification effect is developed based on the multiple exchange amplification principle and amplification features of DNA polymerase Phi29, and the kit has an important applicable prospect and polarization value.

Improved processes for the amplification of target DNA sequences in the form of single or double stranded DNA molecules, especially those present in colony and plaque extracts, using multiple specific and / or random sequence oligonucleotide primers are disclosed along with methods for detecting such amplified target sequences wherein some or all of the deoxyribonucleotides are replaced by deoxyribonucleotide analogues that reduce the Tm of the amplified product. The product of this amplification is used for DNA sequencing and other analyses that involve hybridization. Kits containing components for use in the invention is also described. Also described are further uses of this amplified DNA in sequencing, single base substitution detection, modifying the restriction enzyme fragmentation patterns and other molecular biology applications.

The invention provides a heat-resisting glucamylase which is protein meeting the following condition (a) or (b): (a) the protein contains an amino acid sequence shown as SEQ ID NO.22; and (b) the protein contains a sequence which is at least 70% homologous with the amino acid sequence shown as the SEQ ID NO.22. The coding gene provided by the invention is deoxyribonucleotide meeting the following conditions (1), (2) and (3): (1) the deoxyribonucleotide contains the amino acid sequence shown as SEQ ID NO.20; (2) the deoxyribonucleotide contains the amino acid sequence shown as SEQ ID NO.21; and (3) the deoxyribonucleotide contains a sequence which is at least 705 homologous with the amino acid sequence shown as the SEQ ID NO.20 and the SEQ ID NO.21. The recombinant host cell provided by the invention comprises a recombinant expression vector containing the coding gene. An optimum temperature of glucamylase provided by the invention is higher than 70 DEG C, thermal stability of the glucamylase is good, and industrial application requirement is met. The recombinant host cell provided by the invention is applicable to expression of the glucamylase gene; expression is induced by shaking a flask, wherein expression quantity of KM71 protein is 0.7g / L; and an industrialization application prospect can be achieved.

The invention discloses an antisense oligonucleotide used for inhibiting the expression of micro RNA-485-5p, and an application thereof. The antisense oligonucleotide comprises the sequence of 5'-GAAUUCAUCACGGCCAGCCUCU-3' which can be specifically combined with human miR-485-5p. The antisense oligonucleotide can be ribonucleotide, deoxyribonucleotide or chimera of the ribonucleotide and the deoxyribonucleotide, and can be used for modifying any nucleotide in the chain. The miR-485-5p antisense oligonucleotide can be used for effectively inhibiting the expression of miR-485-5p in a human brainglioma cell U87 as well as the growth and propagation of the human brain glioma cell U87, thus effectively treating the brain glioma and other tumours with high expression of miR-485-5p.

The present application relates to processes resulting in the amplification of ribonucleic acids. The processes comprise the following steps: (a) using a single stranded primer, an RNA-dependent DNA polymerase and deoxyribonucleotide monomers to synthesize a single stranded DNA via reverse transcription of RNA; (b) removing of the RNA; (c) using a single stranded primer comprising a promoter sequence, a DNA polymerase and deoxyribonucleotide monomers to synthesize a double stranded DNA; (d) separating the double stranded DNA into single stranded DNAs; (e) using a single stranded primer comprising a promoter sequence, a DNA polymerase and deoxyribonucleotide monomers to synthesize double stranded DNA on the basis of the single stranded DNA obtained in (d); (f) using an RNA polymerase and ribonucleotide monomers to synthesize multiple single stranded RNAs.

The present invention relates to tolerogenic mammalian dendritic cells (DCs) and methods for the production of the tolerogenic DCs. In addition, the present invention provides a method for enhancing tolerogenicity in a host comprising administering the tolerogenic mammalian DCs of the present invention to the host. The tolerogenic DCs of the present invention comprise an oligodeoxyribonucleotide (ODN) which has one or more NF-kappaB binding sites. The tolerogenic DCs of the present invention may further comprise a viral vector, and preferably an adenoviral vector, which does not affect the tolerogenicity of the tolerogenic DCs when present therein. Enhanced tolerogenicity in a host is useful for prolonging foreign graft survival and for treating inflammatory related diseases, such as autoimmune diseases.

The invention discloses an HCBP 6 (Hepatitis C Virus Core-Binding Protein 6) gene knockout cell line and a construction method thereof. The construction method for the HCBP 6 gene knockout cell line is characterized in that a CRISPER / Cas9 (CRISPR associated protein 9) technology is adopted to construct the HCBP 6 gene knockout cell line, a fragment which conforms to a 5'-N[X]-NGG-3' or 5'-CNN-N[X]-3' sequence arrangement rule in the coding sequence of HCPB6 protein is taken as a target sequence, wherein N presents any one of A, G, C and T, X is greater than or equal to 14 or less than or equalto 30, in addition, X is an integer, and N[X] presents X continuous deoxyribonucleotide. The construction method lays a foundation for HCV (hepatitis C virus) infection mechanism research. The HCBP 6gene knockout constructed by the invention forms frameshift mutation on a genome level, the gene can be transferred to the next generation along with the division and the proliferation of cells, anda stable HCBP 6 gene knockout cell line is formed.

The invention discloses an insertion and deletion site detection marker related to thousand seed weight of wheat and application of the marker. The application of a substance for detecting whether the840th deoxyribonucleotide G of the TaFT-D1 gene on a 7DS chromosome in a wheat genome is deleted or not comprises the following steps: identifying or assisting in identifying thousand seed weights ofwheat; comparing the thousand seed weights of the wheat to be measured; and breeding wheat single plants or strains or lines or varieties with relatively high / low thousand seed weight. The insertionand deletion KASP marker provided by the invention can be used for detecting whether a wheat variety or line contains a locus for increasing thousand seed weight or not. The invention provides a new method for molecular marker-assisted selective breeding of wheat, and the breeding process of high-yield varieties of wheat can be accelerated.

Oligonucleotides directed against the survivin gene are provided for modulating the expression of survivin. The compositions comprise oligonucleotides, particularly antisense oligonucleotides, targeted to nucleic acids encoding the survivin. Methods of using these compounds for modulation of survivin expression and for the treatment of diseases associated with either overexpression of survivin, expression of mutated survivin or both are provided. Examples of diseases are cancer such as lung, breast, colon, prostate, pancreas, lung, liver, thyroid, kidney, brain, testes, stomach, intestine, bowel, spinal cord, sinuses, bladder, urinary tract or ovaries cancers. The oligonucleotides may be composed of deoxyribonucleosides or a nucleic acid analogue such as for example locked nucleic acid or a combination thereof.

The invention provides low-temperature alpha-amylase originated from a fungus. The low-temperature alpha-amylase originated from the fungus is a protein of (a) or (b): (a) a protein which is formed by the following amino acid sequences shown in SEQ ID NO.19; (b) a protein which has the function of the alpha-amylase and is derived from (a) by means of substituting and / or deleting and / or adding one or more amino acid residues to the amino acid sequences shown in the SEQ ID NO.19. The coding gene provided by the invention is deoxyribonucleotide shown in (1) or (2): (1) a nucleotide sequence shown in SEQ ID NO.17; (b) a nucleotide sequence shown in SEQ ID NO.18. A recombinant host cell provided by the invention comprises a recombinant expression vector with the above coding gene. The optimum temperature of the alpha-amylase according to the invention is 40 DEG C which is 10 DEG C lower than that of normal-temperature amylase. The alpha-amylase is good in low-temperature adaptability, still maintains the activity of over 20% of highest activity at 0-30 DEG C and has a huge application prospect in detergent and textile desizing.

A nucleic acid, which is provided in a large amount through a nucleic acid amplification reaction with the use of chimeric oligonucleotide primers, is constructed in a state of containing a modified deoxyribonucleotide for immobilizing the nucleic acid to a solid phase and then immobilized to a solid phase at a high efficiency, thereby giving an immobilized nucleic acid product with excellent qualities.

The invention relates to the technical field of bio-medicine engineering, and provides an aptamer capable of being specifically combined with nodularin-R and an application of the aptamer. A sequencegeneral formula of the aptamer is 5'-AAGGAGCAGCGTGGAGGATA-N40-TTAGGGTGTGTCGTCGTGGT-3', wherein N is any one of the four deoxyribonucleotide basic groups A, T, G and C, the number 40 refers to the number of random basic groups, preferably a sequence represented as anyone from the SEQ ID No.1 to the SEQ ID No.7, most preferably, anyone from the SEQ ID No.9 to SEQ ID No.11. The aptamer can be used for preparing an aptamer sensor or a detection reagent applied to detection of the NOD-R for a drinking water sample. The products also can be a basis for preparing medicines for preventing or treatingNOD-R poisoning and removing the NOD-R from water body or aquatic products.