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Insertion and deletion site detection marker related to thousand seed weight of wheat and application of marker

A thousand-grain weight, wheat technology, applied in the field of insertion and deletion site detection markers, can solve the problem of no allelic variation found

Active Publication Date: 2020-11-06
中国科学院遗传与发育生物学研究所农业资源研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no superior allelic variation in TaFT-1 associated with thousand-grain weight traits has been found

Method used

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  • Insertion and deletion site detection marker related to thousand seed weight of wheat and application of marker
  • Insertion and deletion site detection marker related to thousand seed weight of wheat and application of marker
  • Insertion and deletion site detection marker related to thousand seed weight of wheat and application of marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1. InDel site mining related to wheat thousand-grain weight and development of KASP markers

[0086] 1. Construction of recombinant inbred line population

[0087] The wheat variety Gao 8901 was used as the female parent and 3228 was used as the male parent to cross, and the F 6:9 Generation recombinant inbred line (PG-RIL) population.

[0088] 2. Identification of 1000-grain weight of PG-RIL population under the environment for many years

[0089] The PG-RIL population was planted in the Luancheng Agricultural Ecosystem Experimental Station of the Chinese Academy of Sciences (37 °53'15"N, 114°40'47"E). Each family was planted in 2 rows, the row length was 1.5m, the row spacing was 0.25m, and 30 seeds were planted in each row. Water, fertilizer, and other management for all field trials were performed in accordance with local standards. After the wheat matured, 10 plants were randomly selected from each line of each repetition to investigate the thousand-gr...

Embodiment 2

[0127] Example 2, the establishment of the genotype method for detecting indel sites (TaFT-D1-Ind840) using KASP markers

[0128] With the PG-RIL population family in Example 1 and its parents (Gao 8901 and 3228) genomic DNA as template, utilize the primer KFT-D1 in Example 1 to carry out PCR amplification, obtain amplified product.

[0129] PCR amplification system: DNA template 1.00 μL; primer mixture 0.08 μL; KASP Master Mix (LGC Company, KBS-1016-002) 2.81 μL; ddH 2 O to make up to 5.00 μL.

[0130] Wherein, the final concentration of the upstream primers TaFT-D1-FAM and TaFT-D1-HEX in the primer mixture is 12 μM, and the final concentration of the downstream primer TaFT-D1-C is 30 μM.

[0131] Among them, KASP Master Mix consists of fluorescent probe A, fluorescent probe B, quenching probe A and quenching probe B, and high-fidelity Taq enzyme, dNTP, Mg 2+ and so on. The nucleotide sequence of fluorescent probe A is: 5'-GAAGGTGACCAAGTTCATGCT-3', a FAM fluorescent group ...

Embodiment 3

[0138] Embodiment 3, the application of KASP molecular marker in identification, screening wheat natural population thousand grain weight

[0139] Collect the key wheat parents who have played an important role in my country's wheat breeding since the 1950s, the main varieties planted in different ecological zones in different periods, and some wheat micro-core germplasm materials, and screen the materials with relatively consistent growth periods and normal growth. 133 cultivars / lines formed the natural population.

[0140] From 2013-2014, 2014-2015, 2015-2016 and 2016-2017, the above 133 materials were planted at the Luancheng Agricultural Ecosystem Experimental Station of the Chinese Academy of Sciences (37°53'15″N, 114°40'47 "E), each material planted 3 rows, the row length was 1.5m, 30 seeds were sown evenly, and the row spacing was 25cm. Routine field management, no serious pests and diseases and lodging occurred during the growth period.

[0141] Carry out KASP marker ...

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Abstract

The invention discloses an insertion and deletion site detection marker related to thousand seed weight of wheat and application of the marker. The application of a substance for detecting whether the840th deoxyribonucleotide G of the TaFT-D1 gene on a 7DS chromosome in a wheat genome is deleted or not comprises the following steps: identifying or assisting in identifying thousand seed weights ofwheat; comparing the thousand seed weights of the wheat to be measured; and breeding wheat single plants or strains or lines or varieties with relatively high / low thousand seed weight. The insertionand deletion KASP marker provided by the invention can be used for detecting whether a wheat variety or line contains a locus for increasing thousand seed weight or not. The invention provides a new method for molecular marker-assisted selective breeding of wheat, and the breeding process of high-yield varieties of wheat can be accelerated.

Description

technical field [0001] The invention relates to the fields of biotechnology and molecular breeding, in particular to an insertion-deletion site detection marker related to wheat thousand-grain weight and its application. Background technique [0002] Common wheat (Triticum aestivum L.) is one of the most important food crops in the world, feeding nearly 40% of the world's population (http: / / www.fao.org / ). my country is the world's largest wheat producer and consumer, and the sustainable development of wheat production plays a vital role in national food security and social stability (He Zhonghu et al. (2018) China's wheat industry development and technological progress. Journal of Agricultural Sciences, 8 , 99-106.). Therefore, in the case of limited arable land, increasing the yield per unit area of ​​wheat is the primary goal of Chinese breeders in breeding wheat varieties. The yield per unit area of ​​wheat is affected by the number of ears per mu, number of grains per e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 柳洪安调过李立会麻菲菲许云峰张锦鹏张小涛
Owner 中国科学院遗传与发育生物学研究所农业资源研究中心
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