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SNP label relevant to root characteristics in wheat seedling stage and application

A wheat and root system technology, applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problem of few closely linked markers

Active Publication Date: 2018-03-16
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most genes for root traits are associated with genes related to fertilizer and water use, agronomic traits, and yield traits (Bai et al., 2013), but few closely linked markers have been reported

Method used

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  • SNP label relevant to root characteristics in wheat seedling stage and application
  • SNP label relevant to root characteristics in wheat seedling stage and application
  • SNP label relevant to root characteristics in wheat seedling stage and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Acquisition of SNP marker AX110602569 of genes related to seedling root system

[0045] Yangmai 16 (China Agricultural Development Group Co., Ltd., variety right number: CNA20030436.4) / Zhongmai 895 (Henan Xinda Agricultural Development Co., Ltd., National Review Mai 2012010) DH population was selected, including a total of 200 families including parents.

[0046] figure 1 Seedling root system diagrams of Zhongmai 895 (right) and Yangmai 16 (left).

[0047] 1. Root traits investigation

[0048] The root test adopts the hydroponic method, that is, 30 seeds of each variety are selected, treated with 10% for 20-30 minutes, and washed with sterile water for 5-6 times. select H 2 o 2 The treated seeds were plump and uniform in size, placed in a Petri dish covered with filter paper, and germinated in the dark room of an incubator. For each material, seeds with consistent germination were selected for seedling cultivation, and the culture trays were placed in th...

Embodiment 2

[0079] Example 2, Application of AX110602569 SNP site in detecting wheat root traits

[0080] The tested wheats in this example were 27 offspring varieties of Feiyangmai 16 / Zhongmai 895DH population and Zhongmai 895.

[0081] Among them, 26 offspring varieties of non-Yangmai 16 / Zhongmai 895DH population were the test group, and Zhongmai 895 was the control group; including Zhoumai 30, Huaimai 18, Huaimai 20, Jimai 19, Jimai 21, Jingshuang 16, Jimai 22, Jinmai 45, Liangxing 66, Zhoumai 20, Zhengnong 21, Luomai 26, Zhongjin 13, Yannong 19, Wanmai 38, Wennong 14, Xiaoyan 54, Guangtai 68, Xianmai 12 , Yumai 63, Zhoumai 16, Zhoumai 18, Zhoumai 22, Zhoumai 31, Zhoumai 32.

[0082] 1. Routine detection of root traits at the seedling stage of different wheat varieties to be tested

[0083] The identification of root traits of wheat varieties at the seedling stage was carried out in the greenhouse of the Institute of Crop Science, Chinese Academy of Agricultural Sciences in the winte...

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Abstract

The invention discloses an SNP label relevant to root characteristics in a wheat seedling stage and application. The invention provides a genotypic substance of 19-site deoxyribonucleotide of AX-110602569-7A gene in a detected wheat genome provided in the invention in application of authentication or auxiliary authentication for wheat root characteristics, and the 39cM position of the AX-110602569-7A gene in wheat chromosome 7AS. The invention provides a rated site QRL.caas-7A for root characteristics in the wheat seedling stage and the SNP site of the site for auxiliary sieving of the rated genes in the root system in the wheat seedling stage. Wheat with excellent related root characteristics can be sieved by using the SNP site, and the SNP plays an important role in cultivating water andfertilizer high-efficiency wheat varieties.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a SNP marker related to wheat seedling root traits and its application. Background technique [0002] Cultivating new wheat varieties with high yield, stable yield and good quality requires a stable and well-developed root system and high fertilizer utilization efficiency. Plant roots have dual functions of supporting and fixing plants, as well as absorbing water and nutrients from the soil. At the same time, they are also an important place for the synthesis of various ions, organic acids, hormones and amino acids, and are important contributors to yield formation and sufficient soil nutrients. User (Xu et al., 2015). The growth and physiological characteristics of wheat root system and seedling stage will undergo a series of changes with the effects of irrigation mode and environment. The seedling stage is dominated by vegetative growth, producing tillers and secondary roots, whi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 何中虎杨梦娇王彩荣肖永贵夏先春
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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