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Small molecule polydeoxyribonucleotide and preparation and application thereof

A deoxyribonucleotide and small molecule technology, applied in the field of small molecule polydeoxyribonucleotide and its preparation and application, can solve the problems of cumbersome steps, complex PDRN process, low yield and the like, so as to improve cell activity , Repair damaged cells, high productivity

Inactive Publication Date: 2020-02-04
王超云 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing PDRN preparation technology is complicated, the steps are cumbersome, a large amount of organic solvents and proteases are used in the purification process, and the yield is low
At the same time, the controllability of its preparation and the significant efficacy, targeted efficacy, and efficacy stability of related products still need to be improved.

Method used

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  • Small molecule polydeoxyribonucleotide and preparation and application thereof
  • Small molecule polydeoxyribonucleotide and preparation and application thereof
  • Small molecule polydeoxyribonucleotide and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] A preparation method of small molecule polydeoxyribonucleotide SMPDRN, comprising the steps of:

[0068] (1) Accurately weigh four portions of salmon testis tissue, each of which is 100g, marked as samples 1 to 4, and add alkali lysis solution respectively; add 500mL alkali lysis solution (0.05mol / L EDTA, 0.5mol / L NaOH, 0.1% SDS); Add 500mL alkali lysate (1mol / L EDTA, 5mol / L NaOH, 5% SDS) to No. 2 sample; Add 1500mL alkali lyse (0.05mol / L EDTA, 0.5mol / L NaOH, 0.1% SDS); add 1500mL lysate (1mol / L EDTA, 5mol / L NaOH, 5wt% SDS) to sample No. 4, quickly break up the tissue, mix it upside down, and place sample No. 1 in constant temperature water at 90°C for 60 minutes. Sample No. 2 was placed in 100°C constant temperature water for 15 minutes, No. 3 sample was placed in 90°C constant temperature water for 60 minutes, and No. 4 sample was placed in 100°C constant temperature water for 15 minutes.

[0069] (2) Ice bath to below 40°C, add 0.5mol / L Tris-HCl (pH8.0) to No. 1 s...

Embodiment 2

[0080] A preparation method of small molecule polydeoxyribonucleotide SMPDRN, comprising the steps of:

[0081] (1) Accurately weigh 100g of salmon testis, add 600ml of alkaline lysate (0.1mol / L EDTA, 2mol / LNaOH, 0.5wt% SDS), quickly break up the tissue, mix it upside down, and place it in 95°C constant temperature water for 20min.

[0082] (2) Ice bath to below 40°C, add 2 mol / L Tris-HCl (pH 8.0), the added volume accounts for 1 / 2 of the volume of the reaction system obtained in step (1), and mix by inverting.

[0083] (3) Add 1 mol / L HCL, the added volume accounts for 1 / 5 of the volume of the reaction system obtained in step (2), and mix evenly by inverting.

[0084] (4) Centrifuge at 6000rpm for 15min at 0-4°C, and collect the supernatant.

[0085] (5) Use a DNA fragmentation instrument at 6000 J / s to fragment for 30 seconds.

[0086] (6) Add 10mol / L NH 4 AC solution, whose volume accounts for 1 / 10 of the total volume of the reaction system obtained in step (5), then add...

Embodiment 3

[0094] A preparation method of small molecule polydeoxyribonucleotide SMPDRN, comprising the steps of:

[0095](1) Accurately weigh 100g of salmon testis, add 600ml of alkaline lysate (0.1mol / L EDTA, 2mol / LNaOH, 0.5wt% SDS), quickly break up the tissue, mix it upside down, and place it in 95°C constant temperature water for 20min.

[0096] (2) Ice bath to below 40°C, add 2 mol / L Tris-HCl (pH 8.0), the added volume accounts for 1 / 2 of the volume of the reaction system obtained in step (1), and mix by inverting.

[0097] (3) Add 1 mol / L HCL, the added volume accounts for 1 / 5 of the volume of the reaction system obtained in step (2), and mix evenly by inverting.

[0098] (4) Centrifuge at 6000rpm for 15min at 0-4°C, and collect the supernatant.

[0099] (5) Use a DNA fragmentation instrument at 600 J / s to fragment for 5 minutes.

[0100] (6) Add 10mol / L NH 4 AC solution, whose volume accounts for 1 / 10 of the total volume of the reaction system obtained in step (5), then add -2...

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Abstract

The invention provides a salmon small molecule polydeoxyribonucleotide (SMPDRN) product with a molecular weight concentrated in an efficacy range of the salmon small molecule polydeoxyribonucleotide product, a controllable precise preparation method of the salmon small molecule polydeoxyribonucleotide product, and application of the salmon small molecule polydeoxyribonucleotide product to the fields of cosmetics, medicine, nutritious food and health food. The molecular weight of small molecule polydeoxyribonucleotide is 50-1000 bp, and the mass of the molecular weight of the small molecule polydeoxyribonucleotide at the 50-500 bp fragment accounts for more than 85% of the total mass of the small molecule polydeoxyribonucleotide; and the molecular weight of the SMPDRN is concentrated in thehigh-efficacy range, the pertinence is higher, the efficacy is more stable, and the obtained product has significant effects in improving cell activity, promoting collagen synthesis, regenerating damaged skin, promoting wound healing, tightening skin, increasing skin elasticity, eliminating wrinkles, delaying skin aging, resisting oxidation, preventing formation of color spots, resisting inflammation, repairing damaged cells and other aspects by being verified by cell, animal and human experiment, and is better than an existing PDRN product.

Description

technical field [0001] The invention specifically relates to a small molecule polydeoxyribonucleotide and its preparation and application. Background technique [0002] Deoxyribonucleic acid (DNA) is an important genetic material of organisms. On the one hand, it plays an important role in regulating gene and protein expression, improving cell status, and maintaining normal physiological functions of the body; on the other hand, it serves as a raw material library for The body's growth, development, and repair provide the required deoxyribonucleotides. However, due to the relatively long DNA chain, large molecular weight and charge, it is not suitable for being absorbed by the body through the cell membrane. Researchers in the pharmaceutical, medical beauty, and health care product industries have long been committed to finding DNA biomaterials with a high similarity in base composition to the human body. After the unremitting efforts of researchers, it has been confirmed ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10A61K8/60A61P17/00A61P17/02A61P29/00A61Q19/00A23L33/13
CPCA23L33/13A23V2002/00A61K8/606A61K2800/522A61P17/00A61P17/02A61P29/00A61Q19/00A61Q19/08C12N15/1003C12N15/11C12Q2523/32A23V2200/30A23V2200/302
Inventor 王超云高原董书萍
Owner 王超云
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