The invention discloses a method for breeding thrips palmi karny. The method comprises the steps of cucumber seedling planting, bemisia tabaci introduction, thrips palmi karny imago introduction, subsequent expanding propagation and the like. Honeydew generated by the excreta of bemisia tabaci is adopted as one of nutritional ingredients in the breeding process of the thrips palmi karny, and is more beneficial to population reproduction. The method is simple in process, low in cost and high in breeding efficiency, the bemisia tabaci and the thrips palmi karny are introduced to leaves in sequence, certain population density (not lower than 100 / plant) is kept, cyclic supply of the thrips palmi karny can be achieved, and the obtained thrips palmi karny is high in survival rate and high in vigor. Compared with other conventional indoor thrips breeding, the labor intensity is low, the efficiency is high, and an important insect source guarantee is provided for continuous control study of biological prevention and control of the thrips.