Method for constructing external model dynamically simulating human liver cancer metastasis

Abstract

The invention discloses a method for constructing an in vitro model with dynamic simulation of hepatoma metastasis of human beings, comprising construction of a cell attachment resistant model, construction of the hepatoma cell in vitro metastasis model, identification of the constructed hepatoma in vitro metastasis model, etc. The method of the invention applies high metastasis tendency BEL7402 hepatoma cells to simulate and construct the in vitro model for hepatoma cell metastasis by constructing a model which makes the BEL7402 hepatoma cells deviated from attachment of extracellular matrixes and simulating a condition that transitional cells grows by means of anchorage dependence again when meeting adequate environment. By utilization of the model, control study of carcinoma in situ and tumor during the metastatic process can be performed, and the model can be further taken as a monitoring system for detecting biological behaviors of tumor cells during the metastatic process and judging tumor prognosis. The model has important value in researching a clinical tumor metastatic mechanism and controlling screening of metastasis drugs and also can be taken as a platform for preparing tumor metastasis resistant gene vaccine and establishing a tumor metastasis resistant drug screening technology.

Description

technical field [0001] The invention relates to a method for constructing a liver cancer metastasis model system in vitro, in particular to a method for constructing an in vitro model for dynamically simulating human liver cancer metastasis. Background technique [0002] As a malignant tumor with extremely high morbidity and mortality in China, liver cancer has become a long-term problem for clinicians and scientific researchers because of its rapid disease progression, high degree of malignancy, high metastasis rate, and high recurrence rate. Although a small number of patients have achieved long-term survival due to early diagnosis and treatment, the metastasis and recurrence of liver cancer have always been a major obstacle affecting the treatment effect of liver cancer. Clarifying the mechanism of liver cancer metastasis and its early diagnosis and prevention will be the key to further improve the treatment effect of liver cancer. [0003] Liver cancer is a chronic dise...

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Problems solved by technology

However, this factor has not been taken into account in the current liver cancer metastasis model.

After searching, no papers or patents have been found on the method of constructing an in vitro model for dynamically simulating human liver cancer metastasis and effectively using the in vitro model for dynamically simulating human liver cancer metastasis to conduct dynamic simulation and systematic research on the biological activity of metastatic liver cancer cells to report

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