Flow Chamber for Disturbed Flow Loading of Co-Cultivated Cells

Abstract

The invention provides a flow chamber for loading disturbance flow on co-cultured cells, which comprises a polycarbonate upper plate, a polycarbonate middle plate, a medical silicon membrane, a polycarbonate lower plate and a stainless steel fastener. The polycarbonate upper plate is provided with two co-cultured placement holes; the polycarbonate lower plate comprises an inlet, an inlet fluid buffer slot, an outlet, an outlet fluid buffer slot and a metal joint; the middle of the polycarbonate middle plate is provided with one first rectangular cavity; the middle of the medical silicon membrane is provided with a second rectangular cavity; the inlet fluid buffer slot is communicated with the first rectangular cavity through the second rectangular cavity; and the inlet fluid buffer slot is positioned below the front end of the polycarbonate middle plate and is far away from the first rectangular cavity. In the invention, not only can the influence between two types of relative cells be considered, but also the disturbance and laminar flow can be simultaneously and respectively loaded on the co-cultured cells. The flow chamber is convenient to use, is very easy to disassemble and assemble and is easy to obtain the cells acted on by different flow fields for carrying out subsequent control study.

Description

technical field [0001] The invention relates to a flow chamber in the research field of mechanobiology, in particular to a flow chamber used for co-cultured cells disturbed flow loading. Background technique [0002] Mechanobiology is an emerging interdisciplinary subject developed in the past ten years. It studies the response of organisms to mechanical environments (stimuli) at different levels, especially the research at the level of cells and molecules has attracted more and more attention. The flow chamber is one of the main tools used in vitro to study the mechanical response behavior of cells under fluid shear stress, and is often used in mechanobiology research. [0003] After searching the early technology, it is found that the flow chambers used by scientists are all parallel plate flow chambers, such as the pulsating flow input flow chamber designed by Helmlinger et al. ( Helmlinger G , Geiger RV Schreck S , et al. Efects of pulsatile flow on cultured vascular en...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the shortcomings of existing flow chambers, and provide a simple operation, easy to use, which can not only consider the interaction between two related cells, but also can be used for joint culture of cells with disturbed flow and laminar shear stress respectively. Loaded flow chamber

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