DNA (deoxyribonucleic acid) sequence tag for identifying genetic sex of fenneropenaeus chinensis and application thereof

Abstract

The invention relates to a DNA (deoxyribonucleic acid) sequence tag for identifying the sex difference of fenneropenaeus chinensis and an application thereof. High-throughput sequencing is carried out on mixing pools of DNA of female individuals and male individuals respectively by a high-throughput sequencing means, bioinformatics analysis is carried out on the sequencing results to screen DNA sequence fragments with significant difference in the female and male mixing pools, the obtained differential fragments are verified in the female and male individuals from different sources, finally a DNA sequence tag which can be used for identifying the sex of fenneropenaeus chinensis is obtained, and accurate identification of the genetic sex of fenneropenaeus chinensis can be achieved by utilizing the DNA sequence tag. The method has the characteristics of high efficiency, accuracy and reliability and has an extensive application prospect in fenneropenaeus chinensis sex identification, sex determination and differentiation mechanisms and sex control studies.

Description

technical field [0001] The invention relates to the sex identification and sex control technology of prawns in aquatic biology technology, specifically, a DNA sequence tag for identifying the genetic sex of Penaeus prawns in China and its application. Background technique [0002] Penaeus chinensis is an important cultured shrimp in my country. Because of its large individual size and high nutritional value, it has important economic value. Like other types of prawns, there are obvious differences in the size of male and female individuals, and the growth rate of female individuals in the middle and late stages of growth is much higher than that of male individuals. It is of great significance to increase the production of aquaculture and increase economic benefits. [0003] In order to cultivate the unisexual population of Penaeus prawns, it is first necessary to identify the genetic sex of the prawns. Although the sex identification of male and female shrimp can be reali...

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Problems solved by technology

[0004] In the study of sex markers in prawns, there have been many reports of screening sex markers. Staelense et al. (2008) constructed a high-density genetic linkage map of Penaeus monodon, and found two markers of sex differences from the genetic map, This marker has also been confirmed as a marker for gender identification in other genetically derived Penaeus monodon populations, and the team has also applied for a related patent (sex-specific markers for shrimp and prawns, Chinese invention patent, Application number: 200780015173.9); Zhangetal. (2006) screened a sex-specific microsatellite locus of Litopenaeus vannamei through QTL mapping method, and later confirmed that the microsatellite locus was family-specific, and it was found in individuals from other sources Not applicable; similarly, the study of Alcivar-Warrenetal.(2007) also found sex-specific microsatellite markers of Litopenaeus vannamei, but only in the population studied

However, due to the diversity of different species of prawns, molecular markers of other species cannot be used for sex identification of Penaeus chinensis

In the study of Penaeus prawn, although many linkage maps were constructed, no corresponding sex identification markers were screened (Lietal.2006; Sunetal.2008; Liuetal.2010)

Lietal. (2013) screened a gene differentially expressed between the sexes of Penaeus prawns and developed the marker into a marker that can be used for sex identification of male and female larvae through the method of gene differential expression analysis (Chinese invention patent, publication number: 101709332A), however, this marker is a marker developed at the transcriptome level, which is greatly affected by the growth and development stages of shrimp, and cannot be used for sex differentiation of shrimp before the juvenile stage

DNA markers used for sex identification of Penaeus prawns in China have not been reported

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