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Preparation method and application of silicon carbide@bsa-antimicrobial peptide nanoprobe

A nano-probe and silicon carbide technology, which is applied in the field of forensic science, can solve the problems of complex operation methods, long reaction time, and complicated forensic saliva identification, and achieve the effect of rapid quantitative detection, high sensitivity, and simple preparation

Active Publication Date: 2021-09-07
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention provides a preparation method and application of a silicon carbide@BSA-antimicrobial peptide nanoprobe to solve the problems of complex forensic saliva identification operation methods, long reaction time, and complicated sample preparation in the prior art.

Method used

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  • Preparation method and application of silicon carbide@bsa-antimicrobial peptide nanoprobe

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Embodiment 1

[0040] A fluorescent bacterial sensor based on silicon carbide @BSA-antimicrobial peptide nanoprobe and its application in forensic saliva identification in this embodiment are carried out according to the following steps:

[0041] (1) Preparation of silicon carbide@BSA nano: Add 60mg of silicon carbide powder to 30ml of distilled water, adjust the pH to 9 with ammonia water; add 60mg of BSA to the above silicon carbide solution, and add the mixture to 50ml of poly Tetrafluoroethylene reaction kettle, heated to 140°C, reacted for 650 minutes to obtain a cloudy liquid, dialyzed with a dialysis bag for 3 days, and filtered with a 0.22 μm microporous membrane to obtain a purified silicon carbide @BSA-antimicrobial peptide nanoprobe;

[0042] (2) Preparation of silicon carbide@BSA-antimicrobial peptide nanoprobe-antimicrobial peptide: The prepared silicon carbide@BSA-antimicrobial peptide nanoprobe solution and 0.2mg / mL antimicrobial peptide were coupled by EDC and NHS, and oscilla...

Embodiment 2

[0048] A fluorescent bacterial sensor based on silicon carbide @BSA-antimicrobial peptide nanoprobe and its application in forensic saliva identification in this embodiment are carried out according to the following steps:

[0049] (1) Preparation of silicon carbide@BSA nanometer: Add 20mg of silicon carbide powder to 30ml of distilled water, adjust the pH to 9 with ammonia water; add 80mg of BSA to the above silicon carbide solution, and add the mixed solution to 50ml of poly Tetrafluoroethylene reaction kettle, heated to 140°C, reacted for 650 minutes to obtain a cloudy liquid, dialyzed with a dialysis bag for 3 days, and filtered with a 0.22 μm microporous membrane to obtain a purified silicon carbide @BSA-antimicrobial peptide nanoprobe;

[0050] (2) Preparation of silicon carbide@BSA-antimicrobial peptide nanoprobe-antimicrobial peptide: The prepared silicon carbide@BSA-antimicrobial peptide nanoprobe solution and 0.001mg / mL antimicrobial peptide were coupled by EDC and NH...

Embodiment 3

[0056] A fluorescent bacterial sensor based on silicon carbide @BSA-antimicrobial peptide nanoprobe and its application in forensic saliva identification in this embodiment are carried out according to the following steps:

[0057] (1) Preparation of silicon carbide@BSA nanometer: Add 80mg of silicon carbide powder to 30ml of distilled water, adjust the pH to 9 with ammonia water; add 20mg of BSA to the above silicon carbide solution, and add the mixed solution to 50ml of poly Tetrafluoroethylene reaction kettle, heated to 140°C, reacted for 650 minutes to obtain a cloudy liquid, dialyzed with a dialysis bag for 3 days, and filtered with a 0.22 μm microporous membrane to obtain a purified silicon carbide @BSA-antimicrobial peptide nanoprobe;

[0058] (2) Preparation of silicon carbide@BSA-antimicrobial peptide nanoprobe-antimicrobial peptide: The prepared silicon carbide@BSA-antimicrobial peptide nanoprobe solution and 5 mg / mL antimicrobial peptide were coupled by EDC and NHS, ...

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Abstract

The invention provides a preparation method and application of a silicon carbide@BSA-antimicrobial peptide nanoprobe, which belongs to the technical field of forensic detection methods. The preparation method based on silicon carbide@BSA-antimicrobial peptide nanoprobe comprises the following steps: (1) preparation of silicon carbide@BSA nanometer; (2) silicon carbide@BSA-antimicrobial peptide nanoprobe-antimicrobial peptide preparation. The silicon carbide@BSA-antimicrobial peptide nanoprobe in the detection of oral bacteria and its application method in forensic saliva identification includes: S1: detection of oral bacteria in standard solution; S2: detection of oral bacteria in saliva samples; S3: Identification of forensic saliva samples. The fluorescence sensor based on the silicon carbide@BSA-antimicrobial peptide nanoprobe of the present invention has excellent analytical performance such as high sensitivity, wide linear range, and low cost, and has good practical prospects in the fields of forensic microbial detection and forensic saliva identification and detection.

Description

technical field [0001] The invention belongs to the technical field of forensic medicine, and in particular relates to a preparation method and application of silicon carbide@BSA-antimicrobial peptide nanoprobes. Background technique [0002] Reliably identify the type and source of bodily fluids left at a crime scene, providing important clues for crime scene reconstruction. Forensic saliva testing is an increasingly effective aid in criminal investigations, especially in relation to sexual crimes. Over the past few decades, various saliva identification techniques have been gradually developed. The most common method of identifying saliva is the salivary amylase test. Although salivary alpha-amylase is an enzyme that is abundant in human saliva, it is also present in small amounts in other bodily fluids such as lip mucus, blood, urine, semen and peritoneal fluid, as false positive results can be produced to some extent (M. J. Auvdel, % J Journal of Forensic Sciences, 19...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N33/68G01N33/569C09K11/65
CPCC09K11/65G01N21/64G01N21/6428G01N33/56944G01N33/68G01N2021/6417G01N2021/6432
Inventor 蔡继峰李杏梅丁艳君孟凡明李介男
Owner CENT SOUTH UNIV
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