The invention discloses an ammonia nitrogen on-line monitoring instrument. The ammonia nitrogen on-line monitoring instrument consists of a sample feeding tube, a sample feeding peristaltic pump, a distillation heating bottle, an alkali liquid sample feeding peristaltic pump, a cleaning peristaltic pump, an air pump, a cooling tube, an absorption bottle, a boric acid absorption liquid sample feeding peristaltic pump and a communication pipeline. According to the ammonia nitrogen on-line monitoring instrument, a conduction measuring method for testing ammonia nitrogen, namely a wet process distillation-conductance method, is established. Comparing an analysis result of standard samples, various kinds of surface water and wastewater with a synchronously-adopted national standard analysis result, various technical requirements of monitoring technology standard are met, so that the instrument is easy to operate, time-saving, labor-saving, efficient and correct; the experiment cost is also reduced; and the instrument is suitable for popularization and application of ammonia nitrogen during on-line monitoring.