Method for detecting contents of carcinonitrile, quaternary ammonium salt and ammonium, potassium and calcium impurity ions thereof by adopting cation exchange suppressed conductance method

Abstract

The invention discloses a method for detecting the contents of carcinonitrile, quaternary ammonium salt and ammonium, potassium and calcium impurity ions thereof by adopting a cation exchange suppressed conductance method. The quaternary ammonium salt is N-(3-chloro-2-hydroxypropyl)-N,N,N-trimethylammonium chloride; the sample injection volume of a sample is 25[mu]L; after sample injection thereof, the sample is brought by an eluent into an IonPac CS 17 (4mm*50mm) guard column and an IonPac CS 17 (4mm*250mm) analytical column which are connected to an ion chromatograph for separation; 2mmol / Lof methanesulfonic acid is used as the eluent; at a flow rate of 1.0mL / min, conductance detection shows that the separation degrees of the carcinonitrile, 3-chloro-2-hydroxypropyltrimethylammonium chloride, NH4<+>, K<+> and Na<+> are greater than 1.5. By the method, the overall stability and the reproducibility are ideal and determination data are accurate and reliable; the method can be widely applied to food production, biomedicines and other industries.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for detecting carnenitrile, quaternary ammonium salts, and impurity ammonium, potassium, and calcium ion contents using a cation exchange-inhibited conductometric method, wherein the quaternary ammonium salt is N-(3-chloro- 2-Hydroxypropyl)-N,N,N-trimethylammonium chloride. Background technique [0002] (R)-3-cyano-2-hydroxy-N,N,N-trimethyl-1-propylammonium chloride, commonly known as carnenitrile, is mainly used in the production of L-carnitine and its derivatives, and L-carnitine Alkali is a kind of amino acid that promotes the conversion of fat into energy. It is widely found in various tissues of the human body. It is often added to infant milk powder and athlete's food, because it can transport fat to the mitochondria for burning. It can also be used as an important nutritional supplement for the human body. N-(3-chloro-2-hydroxypropyl)-N,N,N-trimet...

AI Technical Summary

Problems solved by technology

High-performance liquid chromatography-mass spectrometry uses expensive equipment and high cost; the titration end point of the titration method is difficult to observe and determine, and the detection limit is high and the operation is cumbersome; the spectrophotometric method is only applicable to a single quaternary ammonium salt or Total analysis of compound quaternary ammonium salt

At present, there is no effective and reliable method that can simultaneously determine the content of the above substances, so it is urgent to develop a method that can simultaneously detect the content of carnenitrile and quaternary ammonium salt products in plants and the content of impurities such as ammonium, potassium, and sodium ions.

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