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System and method for detecting polymerase chain reaction (PCR) process through non-contact conductivity method in rear time

A non-contact, real-time detection technology, applied in the field of electrochemical sensing, to achieve the effects of improved sensitivity and resolution, shortened time, and long service life

Active Publication Date: 2012-07-25
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In addition, there is no problem of air bubbles on the electrode surface caused by direct contact.

Method used

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  • System and method for detecting polymerase chain reaction (PCR) process through non-contact conductivity method in rear time
  • System and method for detecting polymerase chain reaction (PCR) process through non-contact conductivity method in rear time
  • System and method for detecting polymerase chain reaction (PCR) process through non-contact conductivity method in rear time

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Embodiment Construction

[0022] Specific embodiments of the present invention will be described below in conjunction with the accompanying drawings.

[0023] figure 1 Shown is a schematic view of the structural device of the entire system of the present invention. The system includes an integrated PCR microchip (in the dotted line box), AC excitation power supply, current-to-voltage and amplification circuit, temperature control circuit (sensor signal conversion and amplification and heating control), digital-to-analog / analog-to-digital conversion interface (DAQ) and The Labview control center of the upper computer.

[0024] Among them, the integrated PCR microchip structure integrates modules such as micro reaction chamber (B), temperature sensor electrode and heating electrode (C), electrochemical detection electrode (D) and so on (such as figure 2 shown). figure 2 Among them, (A) is a schematic diagram of the structure, and this size is not the actual size, and the actual integrated PCR microc...

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Abstract

The invention relates to a system and a method for detecting a polymerase chain reaction (PCR) process through a non-contact conductivity method in rear time. The system is characterized by comprising an integrated PCR microchip based on micro-electromechanical system (MEMS) integration, an alternating-current excitation power supply, a current-to-voltage converting and amplifying circuit, a temperature sensing circuit, a heating circuit, a digital-analog / analog-digital conversion interface DAQ and an upper Labview control center, wherein the integrated PCR microchip integrates a micro reaction cavity, a temperature sensing electrode, a heating electrode and an electrochemical detection electrode; the micro reaction cavity is etched on the front surface of a silicon substrate; the heating electrode and the temperature sensing electrode are integrated on the back surface of the silicon substrate; the electrochemical detection electrode is an interdigital electrode, is bonded with the silicon substrate through an insulating layer and forms a sealed structure with the micro reaction cavity; and temperature circulation required by amplification is realized through the temperature sensing electrode and the heating electrode which are arranged on the back surface of the silicon substrate. The sensitivity and the resolution ratio of the system provided by the invention can reach fg / muL below, the reusability of the electrodes is high, the service life is long, and the reliability of the detecting result is high.

Description

technical field [0001] The invention relates to a real-time detection system and method for realizing a polymerase chain reaction (PCR) process by a non-contact conductometric method, including a polymerase chain reaction (PCR) microchip, a non-contact electric conductivity chemical detection electrode, a high-sensitivity readout A circuit and an analysis method, etc., belong to the technical field of electrochemical sensing. Background technique [0002] The rapid detection and analysis of pathogenic microorganisms is an urgently needed technology in the fields of food safety, environmental monitoring, and public health. Real-time fluorescent quantitative PCR technology provides an effective tool for the detection and analysis of microorganisms. Nucleic acid can be quantified during the amplification process, thereby reducing the time required for conventional endpoint detection methods. However, all current real-time quantitative PCRs are based on optical detection method...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12Q3/00C12Q1/68
Inventor 金庆辉方新心景奉香张洹千赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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