Method of improving detecting sensitivity of micro-fluidic chip by non-contact conducting method

Abstract

The invention provides a method of improving detecting sensitivity of a micro-fluidic chip by a non-contact conducting method. The method comprises the following steps: taking the micro-fluidic chip and adding a buffer solution, wherein a sample pond, a sample waste liquor pond, a buffer solution storage pond, a buffer solution waste liquor pond, a feeding channel and a separating channel of the micro-fluidic chip are filled with the buffer solution; absorbing the buffer solution in the sample pond and the buffer solution storage pond; adding a to-be-tested sample solution prepared by the buffer solution into the sample pond and adding the buffer solution containing ethanol into the buffer solution storage pond, wherein the volume content of ethanol is greater than 10%; inserting an electrode, feeding the sample by taking the sample pond as an anode and the sample waste liquor pond as a cathode, and separating the sample by taking the buffer solution storage pond as an anode and the buffer solution waste liquor pond as a cathode after sample feeding; and repeating sample feeding and separating for at least one time after sample feeding and separating. By adopting the detection method, the detection limit can be lower to 10 pg / ml. The method is very high in sensitivity, and can achieve micro-fluidic chip well. In addition, the method is simple to operate, low in cost and high inpracticality.

Description

technical field [0001] The invention relates to the field of microfluidic chip detection, in particular to a method for improving the detection sensitivity of the microfluidic chip non-contact conductance method. Background technique [0002] Miniaturization and integration are important directions for the development of modern analytical instruments. Microfluidic chips have the characteristics of small size, less consumption of samples and reagents, fast analysis speed, simple sample processing, and high separation efficiency. Microfluidic chips can be highly integrated, and the entire analysis process of sample injection, reaction, filtration, separation, and detection can be realized on a chip of a few square centimeters. [0003] The detectors used in microfluidic chips mainly include: ultraviolet-visible light absorption detectors, fluorescence detectors, laser-induced fluorescence detectors, chemiluminescence detectors, conductivity detectors, amperometric detectors, ...

AI Technical Summary

Problems solved by technology

However, these methods have very limited improvement in sensitivity

Moreover, these methods are also accompanied by the problems of high cost and cumbersome operation.

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