85 results about "Cell movement" patented technology

A method and apparatus are provided for receiving system information during inter-cell movement of a user equipment (UE) in a network sharing (NS) system. The UE moves from a previous cell to a new cell. After the movement, the UE compares a public land mobile network (PLMN) identity indicating a PLMN to which the previous cell belongs, with a PLMN identity indicating a PLMN to which the new cell belongs. If the PLMN identity of the new cell is equal to the PLMN identity of the previous cell, the UE sets system information blocks that it has received and stored in the previous cell, as system information of the new cell. If the PLMN identity of the new cell is not equal to the PLMN identity of the previous cell, the UE receives system information blocks with ‘Area scopes Cell and PLMN’ from the new cell, considering that the system information for the previous cell will be invalid in the new cell.

The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well; and a second layer configured to be placed in fluid-tight contact with the upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well.

The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well; and a second layer configured to be placed in fluid-tight contact with the upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well.

A method, system, and computer program product for multi-patterning lithography (MPL) aware cell placement in integrated circuit (IC) design are provided in the illustrative embodiments. A global phase of cell movement is performed. A local phase cell movement is performed, wherein the local phase includes moving a color instance of the cell from a plurality of color instances of the cell within a row of cell in the IC design, wherein the global phase and the local phase are each performed before a final placement is produced for the IC design.

The invention discloses a unicellular micro-operation device used for microinjection. An air supply and a pressure control valve of the device and a left and a right end effectors form a sealed air course through a passage in a holder; the left and the right end effectors are respectively connected with a left and a right three dimensional micromotion stages through the holders; the left and the right three dimensional micromotion stages are symmetrically arranged on the left side and the right side of an inverted microscope worktable; the left and the right end effectors form a unicellular micro-operation flow field with controllable flow rate distribution on the inverted microscope worktable; and a computer collects signals of an image detecting and processing unit to respectively control the pressure control valve and the left and the right three dimensional micromotion stages. The micro-operation device effectively controls the operation environmental flow field of cells, and forms a stable unicellular micro-operation flow field with controllable flow rate distribution to drive the cells to move. Through the invention, the cellular angle in the three dimensional orthogonalized plane can be randomly adjusted, so that the three degrees of freedom (DOF) attitude of the cells can be accurately controlled; and the three dimensional spatial location of the cells is adjusted to realize the accurate positioning of the location and attitude of the cells.

The invention relates to a tumor cell migration dynamics monitoring method based on a microfluidic chip. The method particularly aims at the process of tumor cells migrating and moving into three-dimensional substrates from two-dimensional planes, the microfluidic chip mainly consists of a cell inlet pool (1), a collagen inlet pool (2), a waste liquid pool (3), cell culture rooms (4) and cell migration rooms (5), wherein the cell inlet pool (1) is connected with the upper parts of the cell culture rooms (5), the waste liquid pool (3) is connected with the lower parts of the cell culture rooms (5), and one cell culture room is connected with three cell migration rooms. The tumor cell migration dynamics monitoring method based on the microfluidic chip has the characteristic that the real-time tracking on the cell movement can be realized, and meanwhile, the accurate positioning at the beginning of the cell movement can be realized.

A method, system, and computer program product for multi-patterning lithography (MPL) aware cell placement in integrated circuit (IC) design are provided in the illustrative embodiments. A global phase of cell movement is performed. A local phase cell movement is performed, wherein the local phase includes moving a color instance of the cell from a plurality of color instances of the cell within a row of cell in the IC design, wherein the global phase and the local phase are each performed before a final placement is produced for the IC design.

The invention relates to an automatic cell tracking method for a video microscope image. The existing tracking method has lower degree of automation, and can not adapt to the complex polytropism of the cell movement form and the multi-cell movement tracking. The invention comprises the steps that the abstained video microscope image of the cell movement is enhanced frame by frame; and a target cell is extracted form the enhanced cell movement image to establish the dynamic model of the cell movement for tracking moving target cell. On the basis of analyzing the characteristics of the cell movement and the noise and interference characteristics in the image, the invention adopts the method of dynamic system modeling and stochastic modeling, and through recursing Bayesian filtering and the data connectivity technology, to track the movement trajectory of the cell, and thereby, the automation degree is high, as well as the processing capability is strong.

The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

Disclosed herein are methods for analyzing cell kinematics in a nucleated cell culture from a time-series sequence of multiple fluorescence microscopic images of the nucleated cell culture. The method includes the steps of, (a) identifying every cell nucleus in each fluorescence microscopic image; (b) identifying every cell cluster using the cell nuclei identified in the step (a); and (c) tracking the cells and / or cell clusters using the cell nuclei and cell clusters identified for the fluorescence microscopic images in steps (a) and (b) respectively.

The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well; and a second layer configured to be placed in fluid-tight contact with the upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well.

The invention discloses an efficient microinjection device which comprises a quartz substrate and an injection body. The injection body is fixed on the quartz substrate and provided with an input microchannel, an output microchannel and a cell feeding microchannel, and the portion, between the input microchannel and the output microchannel, of the injection body is provided with a microinjection channel and a cell movement control channel; the microinjection channel is arranged in the vertical direction, perpendicularly communicated with the cell feeding microchannel and fixedly provided with a microinjection needle, a gap is formed between the microinjection channel and the microinjection needle, and the size of the gap is smaller than the diameter of the cells; a limit protrusion is arranged in the microinjection channel, and the cell movement control channel is perpendicularly communicated with the microinjection channel and located over the limit protrusion. According to the efficient microinjection device, cell movement is indirectly controlled by controlling microfluid movement to enable the cells to run into the microinjection needle by themselves, and therefore microinjection operation of the cells is achieved.

The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well; and a second layer configured to be placed in fluid-tight contact with the upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well.

The invention belongs to the technical field of image retrieval and discloses a spatial image retrieval system and a retrieval method based on multi-feature fusion. The spatial image retrieval systembased on multi-feature fusion comprises an input module, a main control module, an image detection module, a feature extraction module, a similarity measurement module, a feature fusion module, a matching module and a display module. A large gradient operation matrix is adopt by that feature extraction module, more gradient intervals are divide, the calculation of the HOG cell movement is omitted,the calculation amount is greatly reduced, the overall speed is increased by about 4 times, and the method is very suitable for application occasions which require high real-time performance. At thesame time, the matching module classifies the fused feature images of heterogeneous image blocks instead of the cascaded feature vectors, which is helpful to improve the performance of the network. The heterogeneous image matching method based on depth learning provided by the invention is not only superior to other methods in performance, but also superior to other methods in training efficiency.

The invention provides an imaging flow cytometer and relates to the field of optical instruments in biology and medical science. The problems that an existing imaging flow cytometer system is low in energy use rate in defocusing distance and speed measurement, complex in structure and high in cost are solved. A polarization optical system is introduced, richer biological information relative to samples to be measured can be acquired, and the imaging flow cytometer is mainly composed of a sample injection unit, a laser source, a speed measurement-focusing unit, an imaging unit and a central control unit. Meanwhile, based on the laser back scattering light spot imaging principle, the speed detection function of the samples to be measured and the automatic focusing function of the cytometer are finished. Through analysis of sequence signals of light signal intensity of the samples to be measured in different sensing positions of an array optical sensor and analysis of distribution of light signal intensity of the detection face of the sensor, the cell movement speed and the defocusing distance can be acquired at the same time, measurement is more accurate and visual, the energy use rate and the signal to noise ratio are higher by the adoption of a laser back scattering mode, and a single optical path detection structure is simpler.

Embodiments of the invention include Optical Cell Guidance (OCG) methods and apparatus to control cell growth. This system guides the leading edge of motile cells with an optical gradient, which biases the cell's motion into the light by pulling on proteins, which act like soft dielectrics in the electromagnetic field. OCG differs from those devices described above in that it controls the direction of cell motility. This is an entirely new field, and the first device to directly manipulate cell motility. OCG differs from current approaches in that it does not trap or hold particles. Instead of trapping and pulling the cell, the goal of OCG is to influence, direct, and control the growth of a growth cone.

The present invention is directed to methods for evaluating the efficacy of a cancer treatment for (i) inhibiting metastasis in a subject, (ii) inhibiting local cancer cell movement, and (iii) inhibiting cancer cell proliferation. The present invention is further directed to methods for monitoring cell motility in a subject. The present invention is also directed to kits for performing any of the above methods.

The invention provides a conducting resin coating device and a lamination assembly production device. The conducting resin coating device comprises conducting resin coating assemblies, each conductingresin coating assembly comprises a conveying part, a cell movement part and a conducting resin coating mechanism, the conveying part is used to convey cells to the cell movement part, the cell movement part comprises an elevating part, a transfer part and a cell suck-up part, the elevating part drives the cell suck-up part to rise and fall, the transfer part drives the cell suck-up part to transfer in the horizontal plane, the cell movement part can suck up the cells and convey the cells between the transfer part and a cell reception station, the conducting resin coating mechanism comprises arotation device and first and second supporting desks, the rotation device can drive the first and second supporting desks to exchange positions during rotation so as to convey the cells, the first and second supporting desks can receive the cells conveyed by the cell movement part in the cell reception station, and the conducting resin coating mechanism coats the cells with conducting resin.

The present invention provides a microfluidic device and its use for cell motility classification. The microfluidic device comprises a fluid inlet, a sample inlet and a channel connecting the fluid inlet and the sample inlet, wherein the channel comprises at least two sections of different sizes, and the channel allows fluid flow from the fluid inlet to the sample inlet.

The invention provides a cell bidirectional invasion monitoring method based on a micro-fluidic chip. According to the cell bidirectional invasion monitoring method, the micro-fluidic chip is adopted for monitoring the cell bidirectional invasion, the inducing or inhibiting effects of different cells or factors on the invasion of a target cell can be simultaneously observed and compared, and the inducing or inhibiting effects of one certain cell or factor on the invasion of different target cells also can be simultaneously observed. The micro-fluidic chip is mainly composed of three main channels and two collagen channels, wherein the three main channels used for cell culture horizontally communicate with the two collagen channels used for the cell migration observation; the left of the middle main channel (1) is connected with the left collagen channel (2), the right of the middle main channel (1) is connected with the right collagen channel (4), the left of the left collagen channel (2) is connected with the left main channel (3), and the right of the right collagen channel (4) is connected with the right main channel (5). The method has the feature of tracking the cell movement in real time, the accurate locating for the cell movement is realized, and meanwhile, the cell invasion capacity and selectivity can be observed and compared.

Netrin proteins and their receptors regulate cell and axon migration, and are implicated in tissue morphogenesis, tumorigenesis and angiogenesis. Deregulation of mechanisms that control cell motility plays a key role in tumor progression by promoting tumor cell dissemination. Unwanted neovascularization also contributes to tumor progression and metastasis and to ocular diseases which are a leading cause of blindness. Here, we describe novel netrin-derived polypeptides and fragments or derivatives thereof that selectively inhibit cell growth, migration or branching. Methods and compositions for the treatment and prevention of conditions involving cell migration or neovascularization, such as cancer and ocular disease, are also provided.

The invention discloses a 6T & 6TPPNN unit layout method based on minimum width constraint. The 6T & 6TPPNN unit layout method comprises the following steps: considering unit alignment of Vdd / Vss track alignment constraint; Performing unit clustering based on minimum width constraint; Performing 6TPPNN unit conversion; performing cell movement based on the fragment effect; According to the methodprovided by the invention, on the premise of not increasing any chip design area, the line length increase caused on the basis of reducing all minimum width constraints can be reduced, meanwhile, thefragment effect is reduced, and the circuit performance is improved.

An optimal input design method and apparatus to achieve rapid broadband nanomechanical measurements of soft materials using the indentation-based method for the investigation of fast evolving phenomenon, such as the crystallization process of polymers, the nanomechanical measurement of live cell during cell movement, and force volume mapping of nonhomogeneous materials, are presented. The indentation-based nanomechanical measurement provides unique quantification of material properties at specified locations. Particularly, an input force profile with discrete spectrum is optimized to maximize the Fisher information matrix of the linear compliance model of the soft material.