33 results about "Cell behaviour" patented technology

This disclosure provides for a three-dimensional (3D) microenvironment presenting defined physical or mechanical cues that regulate cellular behavior and use of the matrix. The disclosure also provides for devices and methods for screening for optimal combinations of physical and mechanical cues in order to create a microenvironment that can regulate specific cellular behavior such as cell growth, proliferation, migration or differentiation.

The invention relates to an orientation material based on magnetic nanofibers and a preparation method and an application of the orientation material based on the magnetic nanofibers, a magnetic nanomaterial and a nanofiber material are combined, a mixed solution is cross-linked in a magnetic field and is induced by the magnetic field to form orientation gel, and the orientation material based on the magnetic nanofibers is obtained. The magnetic field is utilized to pretreat the magnetic nanofibers in the gel forming matrix to form the preset orientation structure, then the gel forming matrix is crosslinked and cured to fix the preset orientation structure, the prepared orientation material can be used for studying the relationship between material properties and cell behaviors under the nanometer-micrometer-macroscopic scale, and the regulation and control mechanism of the material on cell behaviors is disclosed.

A method for measuring surface-induced cellular behavior that includes one or more lithographically patterned, functionalizable structures on a substrate, for example gold islands or grooved quartz, in contact with a fluid and in registry with at least one living cell for a plurality of times. The structures' shape, height, pitch and ordering are controlled by the lithographic process, such that the physical cues imparted to the cell by topography can be tuned independently of the chemical biofunctionality which is subsequently imparted via surface chemistry. Cellular behavior data, such as adhesion, migration, differentiation, division, secretion, apoptosis and necrosis, is measured using imaging sensors in relation to the surface topography and surface chemistry for a plurality of times.

The present disclosure relates to a three-dimensional micro-environment structure for controlling cell behavior, a three-dimensional surface and an array for controlling cell behavior, and a method for manufacturing the three-dimensional micro-environment structure.

The invention related to a method of imaging a clonal cell line comprising providing a test animal comprising a marker gene, inducing inheritable activation of said marker in at least one cell of said test animal, wherein inheritable activation is induced in fewer than 1 in 27 cells in the tissue of interest, incubating the test animal, and visualising those clonal cells which express the marker gene as a result of the inheritable activation. In particular the invention concerns-methods where the tissue is epidermis, and wherein the visualisation is by confocal microscopy such as wholemount confocal microscopy. The invention also relates to toxicity and carcinogenicity testing using such methods.

The invention discloses a method for thermally stimulating cultured cells among cells. Firstly, temperature of a water bath is set to be experimental temperature; the water bath is started for heating; when the temperature of the water bath reaches the final experimental temperature, a cell culture plate/flask holding cells which need to be thermally stimulated and a cell culture plate/flask as blank control are respectively placed at slot positions of 2 base frames; the lower part of the cell culture plate/flask is submerged in water, and the upper part of the cell culture plate/flask is opened and is exposed above a water surface; temperature of the inner surface of the cell culture plate/flask as the blank control is monitored by an infrared temperature monitoring system; heating is performed for temperature reaching time, and then heating stimulation is performed for time required for an experiment; and after completion, the cell culture plate/flask holding the cultured cells is quickly taken out, and thus, the precise and instantaneous thermal stimulation experiment of the cultured cells is completed. The method can very well realize precise and instantaneous thermal stimulation treatment on the cultured cells, and can very well meet the experimental needs like accurately observing the influence of thermal stimulation on cell behaviors and functions.

Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells. The mineral coatings bind polynucleotides and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous polynucleotide delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.

The disclosure provides a device for developing organized tissue strips, such as cardiac tissue strips, for high-throughput assays of functional performance and the methods involved in fabricating, assembling, implementing, utilizing and analyzing data from such assays. The disclosure further provides systems for constructing such devices, systems comprising those devices comprising cells and extracellular matrix material for developing organized tissue strips or comprising the devices and organized tissue strips. The disclosure further provides methods for assaying a property of a tissue strip, such as contractile force.

The invention discloses a composition and a method for generating a linear polymer through light-operated RAFT polymerization in living cells. A monomer, a chain transfer agent, a photoinitiator and living cells are incubated together to achieve uptake of the composition by the cells, and then in-situ synthesis of the polymer in the cells is achieved through illumination. The polymer synthesized in the cells is further extracted, represented, tested and compared with a polymer obtained through in-vitro simulation, and it is found that the polymer synthesized in the cells has high molecular weight and narrow molecular weight distribution and belongs to typical polymer products with controllable polymerization. Therefore, the purpose of introducing the unnatural polymer with a controllable structure into the living cell can be achieved, the composition and the structure in the cell can be further artificially regulated, and even the purpose of controlling the cell behavior and the cell function can be possibly achieved.