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Three-dimensional annular cytoskeleton in response to radio stimulation and preparation method and application thereof

A cell scaffold and stimulus-responsive technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve problems such as connection barriers, scaffold transplantation and infection prevention operation difficulties, achieve good electrical conductivity, reduce cell transplantation The effect of less damage and defects

Pending Publication Date: 2020-11-17
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are also obstacles in the connection of conductive hydrogel, conductive fiber, conductive aerogel, etc. to external wires
On the other hand, in the case of mesenchymal stem cell transplantation, wire connection means that both the wire and the power supply need to be fixed in the animal body or on the body surface, which makes it difficult for the transplantation of the scaffold and anti-infection operations

Method used

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  • Three-dimensional annular cytoskeleton in response to radio stimulation and preparation method and application thereof
  • Three-dimensional annular cytoskeleton in response to radio stimulation and preparation method and application thereof
  • Three-dimensional annular cytoskeleton in response to radio stimulation and preparation method and application thereof

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preparation example Construction

[0032] One aspect of the embodiments of the present invention provides a method for preparing a radio-stimuli-responsive three-dimensional ring-shaped cell scaffold, which includes:

[0033] Graphene was grown on a copper / nickel template by chemical vapor deposition to produce a three-dimensional ring-shaped cell scaffold;

[0034] Alternatively, the conductive material and the fiber are blended to form a conductive fiber, and then processed by a template method to obtain a three-dimensional ring cell scaffold;

[0035] Alternatively, the conductive hydrogel and / or conductive aerogel are printed by 3D printing to obtain a three-dimensional annular cell scaffold.

[0036] In some more specific embodiments, the preparation method includes: at least using any one of photolithography, plasma etching, and electroplating to produce a copper / nickel template with a controllable structure, and then using chemical vapor deposition grow graphene on the copper / nickel template, and then u...

Embodiment 1

[0081]A copper / nickel template with controllable structure is prepared by any one of the micro-nano processing methods in photolithography, plasma etching, and electroplating, and then the three-dimensional ring-shaped copper / nickel template with controllable inner and outer diameters is used as the catalyst, in the horizontal tubular CVD 3D circular graphene grown in a furnace. First, the copper / nickel was placed in absolute ethanol and deionized water for 15 minutes to remove surface impurities, followed by immersion in 0.1mol / L dilute hydrochloric acid for 2 minutes, deionized water for 5 minutes for further cleaning, and blown dry with nitrogen. At low flow H 2 The template was heated to 950 °C under high flow Ar gas, and under high flow H 2 and low-flow Ar gas for 10 min, and then exposed to low-flow H 2 , high flow Ar and CH 4 After 5 minutes, the CVD system was finally cooled to room temperature. Place the template grown with graphene in ferric chloride etching solu...

Embodiment 2

[0085] The preparation of three-dimensional ring-shaped cell scaffolds by 3D printing includes: 3D printing is a kind of rapid prototyping technology, which is based on digital model files, using powdered metal or plastic and other bondable materials, through layer-by-layer printing A technique for constructing objects in a manner. Steps: 1. Modeling: manual modeling, use 3D-MAX, Z-Brush and other software to design a three-dimensional ring structure with controllable inner and outer diameters; 3D scanning, scan the three-dimensional ring bracket template with a 3D scanner to generate digital files . 2. Slicing: convert the already built 3D digital model into a walking path recognizable by the 3D printer and the extruded volume of consumables, first load the model into the software, click on the model slice, and send the file to the 3D printer after slicing. 3. Print: Find the file and click to start printing. The device is placed on the 3D printer and the central container ...

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Abstract

The invention discloses a three-dimensional annular cytoskeleton in response to radio stimulation and a preparation method and application thereof. The preparation method comprises the following steps: growing graphene on a copper / nickel template by adopting a chemical vapor deposition method to prepare a three-dimensional annular cytoskeleton; or blending a conductive substance and a fiber to form a conductive fiber, and then performing treatment by adopting a template method to prepare the three-dimensional annular cytoskeleton; or printing conductive hydrogel and / or conductive aerogel by adopting a 3D printing method to prepare the three-dimensional annular cytoskeleton. The prepared three-dimensional annular cytoskeleton is accurate and controllable in size and good in conductivity; and meanwhile the three-dimensional annular cytoskeleton inoculated with the mesenchymal stem cells by using radio stimulation is simple and convenient to use, the trouble of wire connection is solved,the problems of intra-tissue flowing and low survival rate of the mesenchymal stem cells and cell transplantation injury are reduced, and cell behaviors can be controlled.

Description

technical field [0001] The invention belongs to the technical field of new nanometer materials, and in particular relates to a three-dimensional ring-shaped cell scaffold in response to radio stimulation and its preparation method and application. Background technique [0002] Mesenchymal stem cells (MSCs) are an important member of the stem cell family and a type of pluripotent stem cells, which have the characteristics of self-replication, multi-directional differentiation potential, promotion of stem cell implantation, support for hematopoiesis, and immune regulation; MSCs can be specific in vivo or in vitro Under certain induction conditions, it can differentiate into various tissue cells such as fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, myocardium, and endothelium. The seed cells are used for the repair of tissue and organ damage caused by aging and disease. [0003] Graphene is a carbon nanomaterial composed of single-layer or few-layer carbon atom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N13/00C23C16/26C23C16/01D01F1/09
CPCC12N5/0662C12N5/0062C12N13/00C23C16/26C23C16/01D01F1/09C12N2513/00C12N2529/00
Inventor 程国胜韩芳马迅郝莹
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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