The invention discloses a device for preventing tumor spread after bone tumor biopsy, and belongs to the technical field of medical apparatuses. The device comprises a puncture needle, a sleeve and asampling needle; a sampling coil is fixedly connected to the outer wall of the sampling needle, multiple sampling scraping barbs are fixedly connected to the side wall of the sampling coil, a temporary storage cavity is formed in the sampling needle and located at the bottom of the sampling coil, and the puncture needle sleeves the outer wall of the sampling needle. A sealing platform is fixedly connected to the top of the puncture needle, a liquid injection channel is formed in the middle of the sealing platform, a rubber ring is fixedly connected to the end of the liquid injection channel, asealing gasket is fixedly installed at the junction of the sampling needle and the puncture needle, a sleeve sleeves the outer wall of the puncture needle, a positioning device is fixedly connected to the top of the sleeve, a push needle is matched in an inner cavity of the puncture needle, a fixed needle is fixedly connected to the end of the push needle, and a plug sleeves the outer wall of thefixed needle. By means of the device, the puncture depth is conveniently controlled. Examination tissue cannot shed or get lost, and the puncture success rate is increased. The plug absorbs drugs toplug a puncture channel, a needle passage is subjected to bleeding stopping, and tumor spread is prevented.