49results about How to "Simplify experimental procedures" patented technology

The present invention relates to a molecule marking method for a non-head Chinese cabbage late bolting gene, and the method belongs to the molecular biology field. Using the marking primer DBC16 to amplify the DNA of the non-head Chinese cabbage late bolting family or the breeding material, when the late bolting LB fragment of 252bp is amplified out, the existence of the non-head Chinese cabbage late bolting gene is proved. The present invention sieves the non-head Chinese cabbage late bolting family and the early bolting family P120 for the SSR molecule marking, and a late bolting mark LB ofthe non-head Chinese cabbage late bolting gene is obtained. The molecule marking provided by the present invention can be carried out for selecting and breeding the non-head Chinese cabbage late bolting breed, the select efficiency can be improved greatly, the breeding process can be accelerated, and in producing, the present invention can be applied to the purity identifying for the non-head Chinese cabbage late bolting breed.

The invention discloses an immunochromatography assay for quantitatively detecting organophosphorus pesticides. The immunochromatography assay is characterized in that the principle combining the organophosphorus pesticides and acetylcholin esterase is combined with the immunochromatography technology, a monoclonal antibody for resisting electric eel acetylcholin esterase is prepared, the site, combining with the electric eel acetylcholin esterase, of the antibody is identical with the site, combining with the electric eel acetylcholin esterase, of organophosphorus, the monoclonal antibody is used to fix a detection line, the acetylcholin esterase is used to modify an indicator, the immunochromatography assay for quantitatively detecting the organophosphorus pesticides is provided to achieve the multi-residue detection of the organophosphorus pesticides, and the quantitative detection of the organophosphorus pesticides is achieved according to the signal strength of the detection line and a quality control line. The immunochromatography assay has the advantages that the marking of the organophosphorus pesticide indicator and the chemical preparation of protein conjugates are avoided, detection procedures are simplified, human health damage and severe environment pollution caused by the use of standard organophosphorus pesticides are reduced, and the immunochromatography assay is high in detection sensitivity and capable of satisfying the detection requirements of accuracy, quickness and field high throughput.

The invention relates to a preparation method of hollow nanometer nickel spheres, comprising: dissolving a nickel salt in a deep eutectic solvent at 85-100°C, stirring, and then stirring at 110-150°C for 20-50 minutes; Under the condition of stirring, add water dropwise to the above solution, and stir at 110-150°C for 15-30min; under the condition of stirring, keep the temperature at 85-100°C, add the sodium hypophosphite aqueous solution dropwise into the mixing system, and heat after the dropwise addition 0.5‑2h to obtain nanometer hollow nickel spheres. The preparation process of the invention is simple and controllable, the raw materials are easy to obtain, safe and environment-friendly, and the cost is low, and no organic solvent is used in the reaction process, and no toxic substances are discharged, and the invention is suitable for industrial production.

The invention relates to a three-dimensional physical similarity simulation experimental frame with an adjustable angle. The three-dimensional physical similarity simulation experimental frame comprises an external structural frame and an internal frame, wherein two ends of the external structural frame are provided with an angle determining plate, and each angle determining plate is provided with an angle adjusting track; the bottoms of the front side and the rear side of the internal frame are respectively provided with a guide pulley, the rear end guide pulley is movably hinged with the external structural frame, and the front end guide pulley is matched with the angle adjusting track; and the bottom of the external structural frame is provided with a hydraulic cylinder and a support thereof, and the hydraulic cylinder acts on the internal frame. The three-dimensional physical similarity simulation experimental frame has the advantages that: 1, the operation of the frame is simple, more manpower is saved, and the success rate of experiments is improved; 2, the edge of the frame is provided with an angle value, the angle for the experiment can be directly adjusted, and the experimental process is simplified; 3, the periphery of the frame is protected by using a high-strength transparent materialboard, so that the phenomenon is easy to observe, and results are more realistic; and 4, an open-off cut is reserved, so that the experimental procedure can be simplified, and the experiment is more realistic and simpler.