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49results about How to "Simplify experimental procedures" patented technology

{0><}0{>Method for measuring contents of gold and silver in gold mud sample by virtue of fire assay

{0><}0{>The invention relates to a method for measuring contents of gold and silver in a gold mud sample by virtue of fire assay and belongs to a method for measuring a gold mud material by virtue of a fire assay method. <0}{0><}0{>The method comprises the following steps of: burdening and fusing a test sample so as to obtain a proper amount of lead buttons and fragile molten slag; recycling residual gold and silver in the molten slag; separating gold and silver from the lead buttons through cupelling, thereby obtaining gold and silver combination particles; processing and weighing the particles; grinding the gold and silver combination particles into slices; separating gold by use of nitric acid; and weighing, and calculating contents of gold and silver. <0}{0><}0{>The method is capable of improving the experiment accuracy and simplifying experimental procedures, is convenient to operate, and is capable of realizing batch operation for samples and effectively improving the working efficiency.
Owner:CHANGCHUN GOLD RES INST

Efficient accurate continuous measurement method for lead and zinc in lead concentrate and gold concentrate

The invention relates to an efficient accurate continuous measurement method for lead and zinc in lead concentrate and gold concentrate, in particular to a continuous measurement method for lead and zinc in ore analysis. A saturated nitric acid-potassium chlorate solution is used for decomposing samples, PbSO4 deposit is formed by H2SO4 and Pb and separated from the zinc, and the deposit is dissolved by an acetic acid-sodium acetate buffer solution and used for measuring the content of the lead. After the influence of interference elements on filtrate is eliminated by a chemical masking method, the total quantity of the zinc and cadmium is measured by an EDTA (ethylene diamine tetraacetic acid) volumetric method, and the content of the cadmium is measured by atomic absorption and subtracted from the total quantity to obtain the content of the zinc. The efficient accurate continuous measurement method has the advantages that experimental accuracy can be improved, experimental procedures are simplified, operation is facilitated, mass operation of the samples can be realized, and working efficiency is improved.
Owner:CHANGCHUN GOLD RES INST

Kit and method for detecting activity of DNA methyltransferase

The invention discloses a method for detecting the activity of DNA methyltransferase based on single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification. The method comprises the following steps: (1) adding a Dam MTase acted hairpin substrate to a resection reaction buffer solution, and carrying out a cleavage reaction to produce a 24-nt product; and (2) adding the cleavage product to an amplification reaction buffer solution, carrying out a cyclic linkage-dependent strand displacement amplification reaction to cyclically generate an enhanced fluorescent signal, anddetecting the intensity of the fluorescent signal to determine the activity of the Dam MTase. The method can effectively inhibit nonspecific amplification independent of a target and a template / primer, so the background signal is greatly reduced, and the index amplification of the signal is finally achieved. The method has a high sensitivity and a high specificity, and the lower detection limit can reach 4.8 * 10<-6> U / mL.
Owner:SHANDONG NORMAL UNIV

Method for continuously determining zinc content and iron content of zinc concentrate

The invention belongs to the technical field of mineral content analysis, and discloses a method for continuously determining the zinc content and the iron content of zinc concentrate in order to solve the tediousness of two sample dissolving processes needed by individual determination. The method comprises the following steps: decomposing a sample by using hydrochloric acid, nitric acid, ammonium fluoride and sulfuric acid, adding ammonia water, separating the obtained precipitate, processing the obtained filtrate, determining zinc through using a Na2EDTA complexing titration technology, processing the obtained precipitate, and determining iron by using the Na2EDTA complexing titration technology. The method is very convenient to operate. Compared with existing other continuous titration methods, the method using the same standard EDTA solution in the invention has the advantages of simplification of the experiment program, reduction of the experiment cost, increase of the test speed of the sample, effective increase of the working efficiency, few experiment interference factors, and increase of the accuracy.
Owner:CHANGCHUN GOLD RES INST

Fabrication method of cover glass for conidiospore of Ophio cordyceps sinensis and dynamic counting method of produced conidiospore

InactiveCN102676631AOvercome the shortcomings of low spore production and inability to elute and countImprove work efficiencyMicrobiological testing/measurementCover glassInverted microscope
The invention discloses a fabrication method of cover glass for conidiospores of Ophio cordyceps sinensis and a dynamic counting method of produced conidiospores. The method comprises inoculating Ophio cordyceps sinensis at each hole on a solid culture medium containing at least one hole to obtain at least one inoculated hole; arranging a piece of cover glass above each inoculated hole to obtain a cover glass plate; and culturing the cover glass plate until a colony grows, taking 3 pieces of cover glass every a period of time, and counting to obtain the number of conidiospores produced by Ophio cordyceps sinensis at different moments. According to the experiment, the invention simplifies experimental operation, can in-situ observe and count at fixed points through an inverted microscope, and objectively reflects the conidiospore production cycle of Ophio cordyceps sinensis strains.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI

Fluorescence and/or radionuclide containing nanometer kit for detecting specific activated protease of serum and preparation method and application of kit

The invention discloses a fluorescence and / or radionuclide containing nanometer kit for detecting specific activated protease of serum and a preparation method and application of the kit. The kit is prepared from the following components: magnetic nanoparticles, protease-specific cut peptide chain, and fluorescent molecular and / or radionuclide; the preparation method comprises the steps of preparing the nanoparticles, synthesizing the peptide chain, connecting the fluorescent molecular and / or radionuclide and constructing a nanometer platform. The fluorescence and / or radionuclide containing nanometer kit for detecting specific activated protease of serum can be applied to detection of tumor-specific protease of solid tumors such as breast cancer, non-small cell lung cancer, thyroid cancer, liver cancer, ovarian cancer, endometrial cancer, pancreatic cancer, prostate cancer, glioma and melanoma; all components of the nanometer platform are non-toxic and high in biological applicability, protease-specific cut peptide chain has high-sensitive responsiveness to tumor-specific protease and the detection method is rapid, accurate and specific.
Owner:朱高红

Recombinant expression vector, engineering bacteria, preparation method and application thereof f

ActiveCN106701807ASimple and effective purification technology of IL-18Solve the problem of preparing IL-18Senses disorderBacteriaInclusion bodiesGene engineering
The invention relates to the field of gene engineering, and in particular relates to a recombinant expression vector, engineering bacteria, a preparation method and application thereof. Human IL-18 gene engineering bacteria can be effectively expressed by a gene recombination technology, a simple and effective IL-18 purification technology is established, the problem of preparation of a large amount of IL-18 can be solved, and the clinical needs in the future can be met. A stable and simple inclusion body renaturation and IL-18 purification technology is established, and protein purity can reach more than 97%; and an IL-18 activity identification method can be established. The problems of complex IL-18 purification process, low recovery rate and low purity and the like can be mainly solved, and the process has the advantages of being simple, fast, high in recovery rate and high in purity (97%).
Owner:HE EYE HOSPITAL SHENYANG

Determination method for amount of silver in gold-loaded carbon

The invention relates to a method for determining the amount of silver in gold-loaded carbon, which belongs to the method for determining the amount of silver in carbon chemical analysis methods. The sample is batched and melted to obtain lead buttons of appropriate quality (containing gold and silver) and friable slag, and the residual silver in the slag is recovered. The gold and silver composite particles are separated from the lead buttons by ash blowing, and the obtained gold and silver composite particles are weighed after treatment. The gold and silver grains are separated into gold by nitric acid. Silver content can be calculated after weighing. The invention can improve the accuracy of the experiment, simplifies the experiment procedure, is convenient to operate, can realize the batch operation of samples, and improves the work efficiency.
Owner:CHANGCHUN GOLD RES INST

Fluorescent probe based on oxidative damage bases, and test kit and method for directly detecting DNA methylation

The invention belongs to the field of biological analysis, and relates to a fluorescent probe based on oxidative damage bases, and a test kit and a method for directly detecting DNA methylation. The fluorescent probe based on oxidative damage bases is developed for the first time, and DNA methylation of a single site is accurately detected through cyclic cutting signal amplification. hOGG1 enzymecan specifically cut an 8-oxoG base group in an 8-oxoG / 5-mC base pair, but has no activity on the 8-oxoG base group in an 8-oxoG / U base pair. The presence of the target methylated DNA can induce cyclic cleavage of the fluorescent probe with the aid of the hOGG1 enzyme resulting in an enhanced fluorescent signal. The method is high in sensitivity and high in specificity, a single methylation site can be detected, and the lower detection limit reaches 3.458 * 10 <-15> M. And the method can distinguish 0.01% of methylation level in a complex DNA mixed system, and can also detect the DNA methylation level in the genome.
Owner:SHANDONG NORMAL UNIV

Flow speed measurement instrument and measurement method in micro flow

The invention discloses a flow speed measurement instrument in micro flow, which is applied to micro flow experiments for petroleum recovery rate. The flow speed measurement instrument is characterized in that at least three sections of micro speed measurement tubes are horizontally fixed on a fixed board, and one end of a first section of micro speed measurement tube is connected to an inlet tube line. The other end of the first section of micro speed measurement tube is connected with a tee, and a vertical end of the tee is connected with a section increasing plug tube line. The other end of the tee is horizontally connected with a second section of micro speed measurement tube. A scale is fixed along and beside the second section of micro speed measurement tube. The other end of the second section of micro speed measurement tube is connected with a bent tube and a micro speed measurement tube. The end part of the last section of micro speed measurement tube is connected with an outlet tube line. The flow speed measurement instrument has the effects that the instrument can quantitatively measure the seepage speed of the fluid in a micro model, can quantify the instantaneous seepage speed in the micro model, realizes the measurement for the seepage speed under a high pressure condition, and improves the measuring precision of micro model displacement experiments.
Owner:PETROCHINA CO LTD

Nondestructive detecting method for strain silicon heterojunction in insulator

The invention provides a nondestructive examination method of heterojunction of strained silicon on an insulator, which comprises: (1) Experimental arrangement according to X-ray double crystal symmetrical diffraction geometry based on the crystallography structural feature of the heterojunction of the stained silicon to be examined; (2) making use of synchrotron radiation monochromatic light to conduct double crystal symmetrical diffraction on a specimen to acquire a rocking curve, and to acquire the diffraction peaks of the heterojunction of the stained silicon; (3) rotating the specimen by180 degrees around the surface normal to acquire another rocking curve; (4) comparing the double crystal rocking curves before and after the specimen turns by 180 degrees, and determining the correspondence of each of the diffraction peaks and the diffraction structure; (5) adjusting the angle of incidence of the incident ray so that combined diffraction peaks of Si layer appear asymmetric or divided peaks appear; (6) fixing the angles of incidence of the incident ray, and shooting the synchrotron radiation topography image corresponding to the diffraction structure on each diffraction peak. The invention has the advantages of simple and quick experimental procedures, capability of acquiring the spatial distribution of dislocation of the heterojunction of the stained silicon, and relevantcrystallography information without destroying the specimens.
Owner:GUOBIAO BEIJING TESTING & CERTIFICATION CO LTD

Caoutchouc tree anther callus vitrification ultra-low temperature storage method

The invention provides a method for preserving the callus of rubber plant anther with vitrification and super low temperature, comprising the following steps: the anther callus is inoculated to the pre-culture medium, dark culture is carried out for 2 to 3 days with the culture temperature of 25 to 28 DEG C; the anther callus is processed by 60 percent of PVS2 for 10 to 30 minutes at room temperature; after dehydration process, the anther callus is processed by the PVS2 for 20 to 40 minutes at the temperature of 0 DEG C, the stock solution of the PVS2 is removed, then new PVS2 is added, and the anther callus is stocked in liquid nitrogen; and after the anther callus is resurgent, successive transfer culture is carried out, the regeneration plant can be obtained after further embryoid is induced and the mature embryoid is induced to carry out differentiation. The preservation method by liquid nitrogen with vitrification and super low temperature related by the invention has the advantages of simple equipment, simple experiment procedure and good effect and repetitiveness, etc., can preserve the callus of rubber plant anther for long time, obtains the regeneration plant by culturing after unfrozen and creates conditions for the genetic transformation of the rubber plant.
Owner:RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI

Method for measuring content of tungsten and molybdenum in ore

The invention discloses a method for measuring the content of tungsten and molybdenum in ore. After being weighed, a sample is soaked in mixed acid of hydrochloric acid, nitric acid, hydrofluoric acid and perchloric acid, heated and dissolved at 200-230 DEG C, then disgested, and finally dried by distillation into a wet salt shape; finally, a complex is generated from tungsten through an oxalic acid and hydrochloric acid mixed solution. After capacity is fixed, an inductively coupled plasma-atomatic emission spectrometry (ICP-AES) is used for measuring the content of tungsten and molybdenum. By means of the method, the content of the two elements can be measured at the same time, and the problem that at present, the two elements can not be accurately measured at the same time is solved. In addition, by means of the method, high-temperature and strong-alkali operation is avoided, experiment procedures are simplified, experiment cost is reduced, test speed of the sample is increased, and work efficiency is effectively improved.
Owner:CHANGCHUN GOLD RES INST

Method for determining electron transfer rate of liquid/liquid interface

The invention provides a method for determining the electron transfer rate of a liquid / liquid interface, and the method comprises the following steps: through combining a computer simulation method with a thin-layer cyclic voltammetry, based on the liquid / liquid interface electron transfer theory of the thin-layer cyclic voltammetry, carrying out simulation and forecasting on a thin-layer cyclic voltammetry model through numerical simulation firstly so as to obtain the optimal experiment condition parameter; then, applying the optimal experiment condition to a specific experimental operation so as to obtain a series of ideal cathode platform current values; and finally, carrying out linear fitting on the cathode platform current values so as to obtain the precise constant value of the electron transfer rate of the liquid / liquid interface. In the method disclosed by the invention, the optimal experiment condition is determined by taking computer simulation as the guide and then applied to the specific experimental operation, therefore, the method disclosed by the invention is a new operable method which integrates the advantages of the computer simulation method and the thin-layer cyclic voltammetry, therefore, by using the method, the determination on the electron transfer rate of the liquid / liquid interface is more convenient, quick and accurate.
Owner:NORTHWEST NORMAL UNIVERSITY

Three-dimensional physical similarity simulation experimental frame with adjustable angle

The invention relates to a three-dimensional physical similarity simulation experimental frame with an adjustable angle. The three-dimensional physical similarity simulation experimental frame comprises an external structural frame and an internal frame, wherein two ends of the external structural frame are provided with an angle determining plate, and each angle determining plate is provided with an angle adjusting track; the bottoms of the front side and the rear side of the internal frame are respectively provided with a guide pulley, the rear end guide pulley is movably hinged with the external structural frame, and the front end guide pulley is matched with the angle adjusting track; and the bottom of the external structural frame is provided with a hydraulic cylinder and a support thereof, and the hydraulic cylinder acts on the internal frame. The three-dimensional physical similarity simulation experimental frame has the advantages that: 1, the operation of the frame is simple, more manpower is saved, and the success rate of experiments is improved; 2, the edge of the frame is provided with an angle value, the angle for the experiment can be directly adjusted, and the experimental process is simplified; 3, the periphery of the frame is protected by using a high-strength transparent materialboard, so that the phenomenon is easy to observe, and results are more realistic; and 4, an open-off cut is reserved, so that the experimental procedure can be simplified, and the experiment is more realistic and simpler.
Owner:XIAN UNIV OF SCI & TECH

An immunochromatographic method for the quantitative detection of organophosphorus pesticides

ActiveCN106970217BEnables multi-residue detectionEasy to fixBiological testingCholinesteraseMonoclonal antibody
The invention discloses an immunochromatography assay for quantitatively detecting organophosphorus pesticides. The immunochromatography assay is characterized in that the principle combining the organophosphorus pesticides and acetylcholin esterase is combined with the immunochromatography technology, a monoclonal antibody for resisting electric eel acetylcholin esterase is prepared, the site, combining with the electric eel acetylcholin esterase, of the antibody is identical with the site, combining with the electric eel acetylcholin esterase, of organophosphorus, the monoclonal antibody is used to fix a detection line, the acetylcholin esterase is used to modify an indicator, the immunochromatography assay for quantitatively detecting the organophosphorus pesticides is provided to achieve the multi-residue detection of the organophosphorus pesticides, and the quantitative detection of the organophosphorus pesticides is achieved according to the signal strength of the detection line and a quality control line. The immunochromatography assay has the advantages that the marking of the organophosphorus pesticide indicator and the chemical preparation of protein conjugates are avoided, detection procedures are simplified, human health damage and severe environment pollution caused by the use of standard organophosphorus pesticides are reduced, and the immunochromatography assay is high in detection sensitivity and capable of satisfying the detection requirements of accuracy, quickness and field high throughput.
Owner:MEIZHENG BIO TECH CO LTD

Method for calculating initial velocity of explosive collapse fragments of reinforced concrete target plate

The invention relates to a method for calculating the initial velocity of explosive collapse fragments of a reinforced concrete target plate, which is a calculation method obtained by carrying out dimension analysis on physical quantities influencing the initial velocity of the fragments and fitting initial velocity data of the fragments generated by the concrete target plate under the action of an explosive load obtained by a test. The method has the beneficial effects that a method of combining dimension analysis and test is adopted, the calculation process is simple and accurate, a calculation formula can be corrected according to test data and different requirements, guidance can be provided for later-stage tests on the basis of early-stage tests, experiment programs are simplified, and the number of times of tests is reduced.
Owner:INST OF DEFENSE ENG ACADEMY OF MILITARY SCI PLA CHINA

Hydraulic support shifting device applied to physical similarity simulation test

The invention relates to engineering field physical simulation of mine, rock soil and the like and especially relates to a hydraulic support shifting device applied to a physical similarity simulation test. The device comprises one preposition connection portion and a plurality of postposition shifting portions. The preposition connection portion comprises a plurality of first top bearings, a first upper belt, a plurality of first bottom bearings, three fixing components, a lock catch, a first lower belt, a U type fixing component and a first support body. Each postposition shifting portion comprises a plurality of second top bearings, a second upper belt, a plurality of second bottom bearings, a third concave clamping groove plate, a third convex clamping groove plate, a second lower belt, a connection portion and a second support body. The preposition connection portion is plugged with the adjacent postposition shifting portion, and the two adjacent postposition shifting portions are plugged.
Owner:XIAN UNIV OF SCI & TECH

Flow speed measurement instrument and measurement method in micro flow

The invention discloses a flow speed measurement instrument in micro flow, which is applied to micro flow experiments for petroleum recovery rate. The flow speed measurement instrument is characterized in that at least three sections of micro speed measurement tubes are horizontally fixed on a fixed board, and one end of a first section of micro speed measurement tube is connected to an inlet tube line. The other end of the first section of micro speed measurement tube is connected with a tee, and a vertical end of the tee is connected with a section increasing plug tube line. The other end of the tee is horizontally connected with a second section of micro speed measurement tube. A scale is fixed along and beside the second section of micro speed measurement tube. The other end of the second section of micro speed measurement tube is connected with a bent tube and a micro speed measurement tube. The end part of the last section of micro speed measurement tube is connected with an outlet tube line. The flow speed measurement instrument has the effects that the instrument can quantitatively measure the seepage speed of the fluid in a micro model, can quantify the instantaneous seepage speed in the micro model, realizes the measurement for the seepage speed under a high pressure condition, and improves the measuring precision of micro model displacement experiments.
Owner:PETROCHINA CO LTD

Constructing method of insect-killing genetically engineered beauveria bassiana

The present invention discloses a constructing method of insect-killing genetically engineered beauveria bassiana. Primers are designed to amplify an AaIT gene from a pUC57-AaIT vector by PCR, and a pbarGPEl-AaIT vector is constructed by digestion, purification and pbarGPEl; the primers are used to amplify a GpdA-AaIT-TrpC expression cassette; Not1 single-digestion of pGPS3Ben-bbchit1 is conducted, dephosphorylation is conducted, connection with GpdA-AaIT-TrpC digested by the Not1 is conducted, transformation screening is conducted to obtain a pGPS3Ben-bbchit1-AaIT expression vector, and the pGPS3Ben-bbchit1-AaIT expression vector is introduced into agrobacterium tumefaciens competent cells to obtain positive transformant colonies; and a beauveria bassiana spore suspension is mixed with anagrobacterium tumefaciens solution containing the pGPS3Ben-bbchit1-AaIT vector, and the transformed genetically engineered beauveria bassiana is obtained by screening a solid IM culture medium and aCPZ selection culture medium. The constructed genetically engineered beauveria bassiana can prepare a high-efficiency insect-killing biological control agent, has no pollution to the environment and also has a good prevent and cure effect, and is suitable for popularization in the technical field of biological prevention and cure.
Owner:XUZHOU UNIV OF TECH

Method for detecting PCR amplification product

The invention discloses a method for detecting a PCR amplification product. The method is characterized by comprising the following steps: (1) adding a fluorescent dye and a nonionic detergent into aPCR reaction system, and then carrying out PCR amplification, wherein the concentration of the fluorescent dye in the PCR reaction system is 0.1-1X, and the concentration of the nonionic detergent inthe PCR reaction system is 0.1-0.5%; and (2) observing the system obtained in the step (1) by naked eyes in a dark field under irradiation of an ultraviolet lamp, or observing the system subjected tophotographing by a fluorescent imaging system, and judging whether the PCR amplification product exists or not through the color of the system. For PCR identification which is judged by whether reacting occurs or not, the method can be used for direct judging by observation in a PCR tube by using the fluorescent imaging system or naked eyes without gel electrophoresis, so that experimental procedures are reduced, experimental operation is reduced, time is saved, and efficiency is improved.
Owner:JINLING INST OF TECH

A three-dimensional physical similarity simulation experiment frame with adjustable angle

The invention relates to a three-dimensional physical similarity simulation experimental frame with an adjustable angle. The three-dimensional physical similarity simulation experimental frame comprises an external structural frame and an internal frame, wherein two ends of the external structural frame are provided with an angle determining plate, and each angle determining plate is provided with an angle adjusting track; the bottoms of the front side and the rear side of the internal frame are respectively provided with a guide pulley, the rear end guide pulley is movably hinged with the external structural frame, and the front end guide pulley is matched with the angle adjusting track; and the bottom of the external structural frame is provided with a hydraulic cylinder and a support thereof, and the hydraulic cylinder acts on the internal frame. The three-dimensional physical similarity simulation experimental frame has the advantages that: 1, the operation of the frame is simple, more manpower is saved, and the success rate of experiments is improved; 2, the edge of the frame is provided with an angle value, the angle for the experiment can be directly adjusted, and the experimental process is simplified; 3, the periphery of the frame is protected by using a high-strength transparent materialboard, so that the phenomenon is easy to observe, and results are more realistic; and 4, an open-off cut is reserved, so that the experimental procedure can be simplified, and the experiment is more realistic and simpler.
Owner:XIAN UNIV OF SCI & TECH

Recombinant expression vector, engineering bacteria, preparation method and application thereof

ActiveCN106701807BSimple and effective purification technology of IL-18Solve the problem of preparing IL-18Senses disorderBacteriaInclusion bodiesMicrobiology
The invention relates to the field of gene engineering, and in particular relates to a recombinant expression vector, engineering bacteria, a preparation method and application thereof. Human IL-18 gene engineering bacteria can be effectively expressed by a gene recombination technology, a simple and effective IL-18 purification technology is established, the problem of preparation of a large amount of IL-18 can be solved, and the clinical needs in the future can be met. A stable and simple inclusion body renaturation and IL-18 purification technology is established, and protein purity can reach more than 97%; and an IL-18 activity identification method can be established. The problems of complex IL-18 purification process, low recovery rate and low purity and the like can be mainly solved, and the process has the advantages of being simple, fast, high in recovery rate and high in purity (97%).
Owner:HE EYE HOSPITAL SHENYANG

A method for finding zinc finger protein target sites in the genome

The invention discloses a novel method for finding zinc finger protein target loci from a genome. The method comprises the following steps: firstly, building all 9-nucleotide sequences which can be bound with zinc finger proteins at a binding rate of greater than 2 into a pool; and then, designing a program for finding loci which exist at certain intervals in double chains and are matched with corresponding 9-nuclieotide sequences in the pool from the genome in order to obtain corresponding zinc finger protein target loci. Compared with a conventional method, the novel method has the advantages that constructed zinc finger proteins can be directly applied without being detected or screened, so that an experimental procedure is simplified greatly; the cost is reduced; and the efficiency is increased.
Owner:SUN YAT SEN UNIV

Polymerase chain reaction (PCR)-based quick construction method for tandem repetitive sequence and special primer pair and use

InactiveCN102154269BThere will be no reverse connectionSimple methodDNA preparationDNA/RNA fragmentationYeastRepetitive Sequences
The invention discloses a PCR-based quick construction method for a tandem repetitive sequence, a special primer pair and use. The method constructs a low-copy tandem repetitive sequence of target cis-acting elements which are in the same direction by performing PCR amplification of the target cis-acting elements by using a primer pair designed according to the target cis-acting elements. In the method, more than 3 tandems in the same direction are quickly constructed by performing only one PCR, the positive cloning rate reaches 18 percent, and 200 tandems in the same direction can be obtained by performing two PCRs and subcloning once. The low-copy trandems are used for the quick and effective construction of repetitive cis elements in yeast crossing, and the obtained high-copy tandems can be used very widely in the production of high-molecular-weight repetitive proteins.
Owner:HUNAN AGRICULTURAL UNIV

Preparation method for benzo [e] indole compound

The invention discloses a preparation method for a benzo [e] indole compound, and belongs to the technical field of chemical synthesis. According to the preparation method, the benzo [e] indole compound is synthesized at a high yield by taking aromatic aldehyde, 2-naphthylamine and nitro-paraffin as raw materials, taking lewis acid as a catalyst, and taking ethyl alcohol as a solvent under the catalysis of the lewis acid. The preparation method has the advantages that used raw materials are conventional organic reagents which are low in cost, reaction conditions in a catalytic system are mild and easy to control, the catalyst is low in cost and easy to obtain, an experimental operation is simple, green and pollution-free, and product structures are diversified and the like. Moreover, the ethyl alcohol is taken as the solvent; post-treatment is relatively simple; the pollution to the environment is low; the preparation method is beneficial to realization of green synthesis; industrial production is easy to realize.
Owner:SHAOXING UNIVERSITY

Construction method and applications of multifunctional plant binary plasmid pPBEL-BiFC

The invention discloses a construction method and applications of a multifunctional plant binary plasmid pPBEL-BiFC, and belongs to the technical field of gene engineering. According to the construction method, the original multiple cloning site is modified, wherein the original MCS region is knocked out, and the two new MCS regions are introduced, such that the more enzyme digestion sites can beselected; the original GFP is modified, wherein the GFP is replaced, and the YFP-C fragment and the YFP-N fragment are introduced at different positions of the vector and are respectively positioned at two vectors; the HA tag and the MYC tag are introduced, such that the western-blot protein detection can be performed, wherein the protein A is linked to the HA, the protein B is linked to the MYC,and the western-blot analysis is performed by detecting the protein A so as to detect the presence of the protein B, and vice versa. According to the present invention, with the contructed plasmid, the co-immunoprecipitation (Co-IP) reaction can be performed.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
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