The invention relates to a method for determining
chloramphenicol in freshwater pond
sediment by applying a liquid chromatograph /
mass spectrometer. The method comprises the following steps of firstly, collecting a
sediment sample of an
aquaculture pond; secondly, extracting the sample; thirdly, purifying with n-
hexane; fourthly, extracting water saturated
ethyl acetate solution; fifthly, blowing extracting solution to be dry with
nitrogen gas, and dissolving the dry extracting solution with
acetonitrile-water (5+95) to obtain standby solution; sixthly, performing
solid phase extraction column for eluting; seventhly, extracting the water saturated
ethyl acetate solution, and blowing dry with the
nitrogen gas; eightly, dissolving
methanol water (50+50), whirling, filtering by a
microfiltration membrane of 0.22[mu]m and determining by using the liquid chromatograph /
mass spectrometer. According to the method disclosed by the invention, by pretreatment for the freshwater pond
sediment, a matrix disturbing substance is removed, the sensitivity is improved, and the pretreatment process is optimized; in addition, the sample is prevented from being derivaed, so that the operation step is simplified; a reliable detection means can be provided for checking a
pollution source and evaluating the influence of the
ecological environment on healthy breeding.