The invention discloses a method for making a tissue chip for gastric cancer HER-2 FISH, which includes selecting experimental materials, making an experimental tissue chip, dewaxing, removing cross-linking pretreatment, digesting with proteinase K, observing the effect of cell digestion, and repeating digestion and denaturation The integrity of the wax block archives is protected to the greatest extent through the material selection step of "drawing the experimental area first and then cutting the tissue section"; the tissue section area is small, which can save experimental reagents, reduce workload, and improve work efficiency and accuracy. ; Laying multiple tissue sections on one slide can reduce subsequent processing steps, further improve work efficiency, and save archive space; the process of observing the effect of cell digestion has fewer processing steps and shorter time, without immersion oil and counterstaining agent, Reduce costs and avoid the risk of being interfered with by both, and there is no risk of tissue breakage or chip loss. The invention can greatly reduce the material cost and labor cost of experiments, and improve work efficiency and accuracy.