The invention relates to selenium-rich chicken essence seasoning containing wild fungi and a preparation method of the selenium-rich chicken essence seasoning and belongs to the technical field of food additives. According to the selenium-rich chicken essence seasoning containing wild fungi, holy basil, tricholoma matsutake powder, russula vinosa powder, truffle powder, thelephora ganbajun zang powder and an enzymatic hydrolysate form main delicate flavor, then, roxburgh rose juice and Stanleya pinnata are used for correcting the delicate flavor, besides, disodium 5'-ribonucleotide, xylose and low-sodium salt are compounded, so that the chicken essence seasoning has unique flavor, is fragrant and delicious and contains rich nutrients; meanwhile, through compounding of the thelephora ganbajun zang powder and the Stanleya pinnata, the selenium content of the product is greatly increased, and a good health-care function is realized. Besides, the content of fat and protein in the chicken essence seasoning is greatly reduced, worry of an eater about obesity is avoided, and the selenium-rich chicken essence seasoning is easy to popularize and apply.