The invention relates to a feed additive capable of improving the flavor of pork as well as a preparation method and application of the feed additive. The feed additive comprises the following components of phytosterin, a plant extract, selenium yeast, probiotics powder, conjugated linoleic acid, saccharicterpenin and a carrier substrate. The preparation method comprises the following steps of weighing the components in parts by weight, firstly screening the plant extract, and performing uniform mixing; then mixing the phytosterin with a part of the carrier substrate; and finally, adding the remaining components, performing sufficient and uniform mixing, performing granulation, and performing screening. Compared with the prior art, the feed additive disclosed by the invention has the advantages that after the feed additive is added to a pig basic ration, the growth of pigs can be promoted, the feed utilization rate can be increased, the eye muscle area and the lean meat percentage canbe increased, the water drop losses can be reduced, and the content of intramuscular fat of the pork can be adjusted and controlled, so that the intramuscular fat is rich and uniform, the compositionstructure and the balanced proportion of fatty acid in the pork are improved, the fat percentage is reduced, the content of flavored precursor substances of the pork is increased, the tenderness and the juicy nature of the pork are improved, and the pork is delicious in flavor, delicate in mouth feel, fresh and juicy, free from drug residues and healthy.