The invention discloses a preparation method of novel
anticoagulant stents
coating capable of capturing endothelial
progenitor cells, which comprises the following steps: performing
hydroxylation processing on surface of a material, then dissolving
dopamine in a
Tris-base(pH is 8.5, 2mg / ml) solution, then depositing a layer or multilayer
dopamine membrane at temperature of 37 DEG C on the surface of the material, then adjusting reaction condition according to pH and EDC / NHS concentration, and performing covalent
grafting on a mixture of CD133+(0.8-3.2ug / ml) and
Fucoidan(50-400ug / ml) at temperature of 37 DEG C to obtain a target product. The preparation method has the advantages of simple operation, mild reaction condition, high efficiency and low cost. A target
coating has strong
antithrombotic performance, anticoagulation and inhibition of
endometrial hyperplasia of
smooth muscle due to effect of low molecular weight
Fucoidan, can rapidly capture the endothelial
progenitor cells of
peripheral blood so as to induce the endothelial
progenitor cells to differentiate for obtaining endothelial cells, and then a complete endothelial cells layer is formed, and the novel
anticoagulant stents
coating has excellent anticoagulation performance and biological compatibility.