The invention discloses a kit for detecting trace drugs in
sewage by using a time-resolved
fluorescence immunochromatography technology, and an application of the kit. The kit comprises a kit body; and an
aptamer affinity column, a time-resolved fluorescent
microsphere freeze-
drying tube and an
immunochromatographic test strip are arranged in the kit body. According to the kit, a time-resolved fluorescent
microsphere is used for marking an
antibody, the half-life period of
fluorescence is long, the product stability is relatively high, and the normal-temperature storage is facilitated; the
detection limit reaches 50ng / L, so that accurate detection of the drugs is realized; small molecules such as the to-be-detected drugs, namely, methylamphetamine and the like in the
sewage are enriched and concentrated through the
aptamer affinity column, so that the
sewage detection sensitivity of the test strip is improved, and the practical sewage detection sensitivity can reach 5ng / L according tothe effect of concentration by 10 times; the kit has obvious advantages in the aspects of inter-
assay variations and the precision of the test strip, and is sufficient in reaction and higher in sensitivity; the operation is simple and convenient; and the test strip is simple and convenient to use and operate, and does not need to be operated by professionals.