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Bone tissue engineering stent and preparation method thereof

A technology of bone tissue engineering and bone powder, applied in additive processing, medical science, prosthesis, etc., can solve problems such as liver, kidney and other organ damage and adverse reactions

Inactive Publication Date: 2016-01-27
TIANJIN HAIHE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term oral administration of large amounts often causes a variety of adverse reactions, and even damages the liver, kidneys and other organs.

Method used

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  • Bone tissue engineering stent and preparation method thereof
  • Bone tissue engineering stent and preparation method thereof
  • Bone tissue engineering stent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Dissolve PLGA10g (25:75, w / w) PLGA in 100ml of dichloromethane and acetone mixed solution, wherein dichloromethane: acetone (v / v) = 1:1, and make a 10% PLGA solution.

[0031] (2) Add 15 g of 5 μm allogeneic bone powder gradually to 10% PLGA solution in small amounts at 20° C., and stir with sealed magnetic force for 3 hours until a uniform mixed solution is formed.

[0032] (3) 3 g of isoniazid (INH), 1 g of rifampicin (RFP), and 1 g of bone morphogenetic protein (BMP-2) were added to the above mixed solution, sealed and ultrasonically stirred for 1 hour to prepare a spray solution.

[0033] (4) In order to obtain a suitable viscosity for printing, transfer the solution into a syringe and pre-cool at 4°C for 30 minutes.

[0034] (5) Place the pneumatic 3D printer in an ultra-clean bench and irradiate it with ultraviolet light for 30 minutes to sterilize it. Install the pre-cooled injection needle tube filled with printing fluid on the printer frame, and run the pr...

Embodiment 2

[0036] (1) PLGA is dissolved in dichloromethane (DCM) and acetone to form a 10%-25% solution. For example, dissolve 30g (85:15, w / w) PLGA in 200ml dichloromethane and acetone mixed solution, wherein dichloromethane: acetone (v / v) = 1:1, and make a 15% PLGA solution.

[0037] (2) 15g of 7 μm allogeneic bone powder was gradually added to 15% PLGA solution in a small amount at 20° C., sealed and magnetically stirred for 3 hours until a uniform mixed solution was formed.

[0038] (3) 4 g of isoniazid (INH), 3 g of rifampicin (RFP), and 2 g of bone morphogenetic protein (BMP-2) were added to the above mixed solution, sealed and ultrasonically stirred for 1 hour to prepare a spray solution.

[0039](4) In order to obtain a suitable viscosity for printing, transfer the solution into a syringe and pre-cool at 4°C for 30 minutes.

[0040] (5) Place the pneumatic 3D printer in an ultra-clean bench and irradiate it with ultraviolet light for 30 minutes to sterilize it. Install the pre-...

Embodiment 3

[0042] (1) PLGA is dissolved in dichloromethane (DCM) and acetone to form a 10%-25% solution. For example, 30g (50:50, w / w) of PLGA was dissolved in 300ml of dichloromethane and acetone mixed solution, wherein dichloromethane: acetone (v / v) = 1:1, made into 10% PLGA solution.

[0043] (2) 30 g of 7 μm allogeneic bone powder was gradually added to the 10% PLGA solution in small amounts at 20° C., sealed and magnetically stirred for 3 hours until a uniform mixed solution was formed.

[0044] (3) 2 g of isoniazid (INH), 4 g of rifampin (RFP), and 2 g of bone morphogenetic protein (BMP-2) were added to the above mixed solution, sealed and ultrasonically stirred for 1 hour to prepare a spray solution.

[0045] (4) In order to obtain a suitable viscosity for printing, transfer the solution into a syringe and pre-cool at 4°C for 30 minutes.

[0046] (5) Place the pneumatic 3D printer in an ultra-clean bench and irradiate it with ultraviolet light for 30 minutes to sterilize it. Ins...

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Abstract

The invention relates to a bone tissue engineering stent and a preparation method thereof. The bone tissue engineering stent is prepared from PLGA, isoniazide, rifampicin, allogeneic bone power and bone morphogenetic protein at the weight ratio of (87-51) to (1-6) to (1-6) to (10-30) to (1-7); according to the bone tissue engineering stent, polylactic acid-hydroxyacetic acid material, allogeneic bone power, antituberculosis drugs and bone morphogenetic protein are combined by adopting a 3D printing technology, a bioactive stent is manufactured by adopting the 3D printing technology, the physical, chemical and biological properties of the stent are detected, and the bone tissue engineering stent has the good biocompatibility and the good bone-formation promotion capacity and is biodegradable.

Description

technical field [0001] The invention relates to a medical material and a preparation method thereof, in particular to a bone tissue engineering scaffold and a preparation method thereof. Background technique [0002] Bone tuberculosis is a common secondary extrapulmonary tuberculosis, accounting for about 3% of all tuberculosis cases, and about half of them involve the spine. If the diagnosis and treatment are delayed, the patient may be permanently disabled or even die. As the most common form of bone tuberculosis, spinal tuberculosis has also been on the rise with the increase of tuberculosis in recent years. Mycobacterium tuberculosis is sensitive to drugs such as rifampicin (RFI) and isoniazid (INH). Drugs such as rifampicin and isoniazid can penetrate into host cells and have killing and (or) inhibitory effects on Mycobacterium tuberculosis inside and outside the cells. However, long-term oral administration of a large amount often causes a variety of adverse reaction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/54A61L27/50A61L27/58B29C67/00B33Y10/00
Inventor 张文龙王勇韩臣富鲍玉成苗瑞瑞谢祎苏丽洁张汝柄王露
Owner TIANJIN HAIHE HOSPITAL
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