34results about How to "Improve enzymatic hydrolysis and saccharification efficiency" patented technology

The invention discloses a biological-chemical combined treatment process for improving saccharifying effect of lignocellulose. According to the process, a lignocellulose raw material is subjected to biological treatment by virtue of an efficient lignocelluloses degrading bacterium sphingobacterium sp. LD-1 (preservation number is CGMCC No.10920), so as to remove partial lignin and hemicellulose components in the lignocellulose raw material with the action of microorganisms, and to damage the stable structure of the lignocellulose to a certain degree; and furthermore, the lignocellulose undergoes chemical treatment at low temperature by virtue of alkali / urea, so that the lignocellulose is more loose in structure. The lignocellulose, which is preprocessed by the process disclosed by the invention, achieves significant change in component content and obvious increase in proportion of cellulose; in addition, the structure of the lignocellulose is significantly changed as well, and the surface of the raw material becomes rough, loose and porous, so as to greatly increase an accessible surface during enzymolysis saccharifying. The combined pretreatment process disclosed by the invention has the advantages of being not high in requirement on equipment, simple in operation, mild in processing condition, low in cost and the like, and the saccharifying efficiency of the lignocellulose is greatly improved.

The invention provides a high-capacity double-systematic-circulation cellulose enzymolysis reactor and its application. The high-capacity double-systematic-circulation cellulose enzymolysis reactor concretely comprises: a first reaction tank (a) comprising a tank body, shearing homogenizers positioned in the tank body, an inlet used for adding a feed material, and an outlet used for discharging the material; a second reaction tank (b) comprising above two series or parallel reaction tubes; a pipeline (c) connecting the first reaction tank with the second reaction tank; and a pipeline (d) connecting all tubes in the second reaction tank, wherein the number of the shearing homogenizers is two or more; and the volume of the first reaction tank is 3-150t, and preferably 30-100t. The enzymolysis reactor has the advantages of high conversion rate, high concentration, short conversion time, low enzyme preparation consumption, low energy consumption and the like when the enzymolysis saccharification of celluloses comprising fibrilia and the like is carried out by the enzymolysis reactor, so the enzymolysis reactor is especially suitable for high-capacity cellulose enzymolysis reaction systems.

The invention relates to a production process of waxy corn rice wine, which specifically includes the following steps: 1), cleaning waxy corn seeds, beating, and sieving; 2), adding a compound enzyme preparation for enzymatic hydrolysis and saccharification; 3), adding activated corn Yellow rice wine yeast and yellow rice wine koji, add lactic acid to adjust the pH value and then carry out primary fermentation; 4) Add compound aroma-enhancing yeast for secondary fermentation; 5) Filter through diatomite, then filter through a ceramic microporous membrane filter, and finally pass through a tube Type sterilizer high temperature instant sterilization passivation, after sealing enters the aging stage. The invention solves the problem of high content of urethane while improving the production efficiency, ensures rich nutritional value and good taste of rice wine products, and is suitable for large-scale automatic production.

The present invention provides a dual body circulatory reactor for cellulose enzymolysis and an application thereof. Specifically, the present invention provides a reactor for cellulose enzymolysis comprising: (a) a first reaction tank comprising a tank body, a shear homogenizer provided in the tank body, an inlet for feeding, and an outlet for discharging a material; (b) a second reaction tank comprising two or more reaction tubes connected in series or in parallel; (c) a pipeline connecting the first and the second reaction tanks; and (d) a pipeline connecting each tube bodies in the second reaction tank. When conducting enzyme saccharification on bast fiber crops and other celluloses, the enzymolysis reactor of the present invention has advantages of high conversion rate, high concentration, short conversion time, low enzyme consumption, and low energy consumption, and thus is especially suitable for a large volume cellulose enzymolysis reaction system.

The invention discloses a method for strengthening the ammonia pretreatment of waste biomass by means of lignin-degrading bacteria. Particularly, on the basis of the ammonia pretreatment of the waste biomass, the lignin-degrading bacteria, namely Cupriavidus basilensis B-8, with the preservation number of CGMCC No.4240 are used, residual lignin in the waste biomass is further removed by improving culturing conditions, and the cellulose accessible surface is improved in the enzymolysis and saccharification processes. By using the method, the enzymolysis efficiency of the pretreatment of a single ammonia method is improved by about 30%, and the method has the advantages of being short in treatment time, simple in operation, small in secondary pollution, low in cost and the like.