Method for improving specific activity of cellulase and application of method

A cellulase and specific activity technology, applied in the field of cellulase activity modification and biomass saccharification fermentation, can solve the problem of no clear conclusion on the promotion or inhibition mechanism, and achieve the improvement of saccharification efficiency, specific activity, and enzymatic hydrolysis. The effect of saccharification efficiency

Active Publication Date: 2022-02-18
HUBEI UNIV
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Problems solved by technology

The carboxyl and methoxy groups of phenolic acids may be related to the promotion of the enzymatic hydrolysis process, but there is no clear conclusion on the promotion or inhibition mechanism of lignin-derived phenolic compounds on the enzymatic hydrolysis process

Method used

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  • Method for improving specific activity of cellulase and application of method
  • Method for improving specific activity of cellulase and application of method
  • Method for improving specific activity of cellulase and application of method

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Embodiment Construction

[0021] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0022] 1.1 The effect of phenolic acid on the enzymatic hydrolysis efficiency of cellulase

[0023] 1.1.1 Effect of phenolic acid on cellulase hydrolysis

[0024] The effects of phenolic acids on cellulase hydrolysis efficiency were analyzed using phosphoric acid-swelled cellulose and microcrystalline cellulose as substrates. Add 25mM phenolic acid to the enzymatic hydrolysis reaction system to judge the enzymatic hydrolysis efficiency of cellulase by detecting the release amount of reducing sugar after 24 hours of enzymatic hydrolysis. The control group without adding phenolic acid was defined as 100% enzymatic hydrolysis efficiency. In the enzymatic hydrolysis system using phosphoric acid-swollen cellulose as th...

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Abstract

The invention relates to a method for improving the specific activity of cellulase and application of the method. In particular, cellulase with improved specific activity is obtained after cellulase is incubated with phenolic acid, and the cellulase with improved specific activity can be used for improving the biomass enzymolysis saccharification efficiency. Efficient biorefinery of lignocellulosic biomass is limited due to the negative impact of expensive cellulase and lignin degradation products on cellulase hydrolysis. A lignin-derived phenolic acid compound, vanillic acid, syringic acid, ferulic acid, isovanillic acid and the like can effectively improve the specific activity of cellulase, the saccharification efficiency of different biomass is effectively improved under the condition that the enzyme dosage is not increased, and the enzyme use cost of biomass enzymolysis saccharification can be effectively reduced.

Description

technical field [0001] The invention relates to the field of cellulase activity modification and biomass saccharification and fermentation, in particular to a method for improving the specific activity of cellulase and its application. Background technique [0002] Resource, energy and environmental problems are becoming more and more serious. Therefore, there is an urgent need to develop clean and renewable energy sources to replace fossil resources. Lignocellulosic biomass is the most abundant renewable resource on earth and can be converted into high-value chemicals or biofuels through sugar platforms. Commodities produced from renewable resources can replace products based on petroleum supplies, helping to improve energy security and reduce environmental impact. Lignocellulose is mainly composed of cellulose, hemicellulose and lignin. The production of biomass from lignocellulosic biomass involves three steps, namely pretreatment, enzymatic hydrolysis and subsequent f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12P19/14
CPCC12N9/2437C12P19/14Y02E50/10
Inventor 江正兵冉秋萍宋慧婷李华南刘家书周樱
Owner HUBEI UNIV
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