The invention discloses a method for high-flux
assay of
phytase activity in a transgenic
phytase crop seed. The method comprises the following steps: (1) building a
phosphorus standard curve based on a
phosphorus content as a horizontal coordinate and a light absorption value as a
vertical coordinate; (2) leaching out
phytase in a sample to be assayed so as to obtain leach liquor of phytase; (3) carrying out
enzyme activity
assay reaction of phytase on the leach liquor of phytase in a microplate with 96 holes; and (4) assaying the light absorption value of a reaction solution, calculating the
phosphorus concentrations of various samples to be assayed according to a
linear regression equation corresponding to the phosphorus
standard curve, calculating the phytase activities of the samples to be assayed according to an
enzyme activity calculation formula. Accuracy and sensitivity testes show that the assayed result obtained by using the method has good accuracy, small error, high stability and good
repeatability, and
coefficient of variation of the assayed result obtained by using the method is far lower than that obtained by the existing microplate method. The
assay method disclosed by has the advantages of
high flux, high assay efficiency, low cost, high accuracy and the like, and is easy to operate.