The invention discloses a preparation method of a rice-lobster mixed culture slow-release fertilizer for reducing diseases and insect pests, wherein the preparation method includes the following steps: mixing coconut shell charcoal, calcined kaolin, vermiculite powder and sepiolite, adding the mixture into a sodium hydroxide solution, soaking, filtering, washing and drying, adding urea, monoammonium phosphate, calcium triple superphosphate, potassium dihydrogen phosphate, a potassium magnesium sulfate fertilizer, EDTA chelated zinc, EDTA chelated manganese, sodium selenite and boric acid, andmixing evenly to obtain a premix a; mixing fermented tea seed meal, peat soil, bagasse, corncob powder, corn germ meal and oat bran, adding water, and adding an EM microbial agent, and fermenting to obtain a premix b; and mixing the premix a, the premix b and gelatin evenly, adjusting the pH value of the system to 6.4-6.9 by hydrochloric acid, adding high-temperature-resistance alpha-amylase, stirring at room temperature, carrying out enzyme deactivation, adding a slow-release enhancer and glutaraldehyde, stirring, carrying out spray drying, granulating, and drying to obtain the rice-lobster mixed culture slow-release fertilizer for reducing the diseases and insect pests.