The invention provides a thermophilic alkaline
pectin lyase gene, an
engineering bacterium, thermophilic alkaline
pectin lyase and application thereof and belongs to the technical field of
biological engineering. The preservation number of the
engineering bacterium is CGMCC No.11460, the
engineering bacterium is named as
Escherichia coli through classification, the depository authority is the General
Microbiology Center of China Committee for Culture Collection of Microorganisms, and the preservation date is September 29, 2015. The invention mainly discloses the thermophilic alkaline
pectin lyase, the optimum
reaction temperature is 70 DEG C, the optimal Ca2+ concentration is 0.8 mM, a substrate is 0.2% (w / v) polygalacturonic acid, and pH is 9.5. The
specific activity of the lyase is 900 U / mg under the optimal conditions that the pH is 9.5, the Ca2+ concentration is 0.8 mM and the temperature is 70 DEG C; heat preservation is performed at the temperature of 70 DEG C, and a half-life period is 6 h; after
nickel column purification treatment is performed, CbPelC can be up to 50 mg / L (1 L fermented liquid). The
heat resistance and yield of the thermophilic alkaline
pectin lyase are remarkably improved, the thermophilic alkaline
pectin lyase more adapts to the modern industrial production requirements, and an
effective method is provided for industrial
mass production of the alkaline
pectinase.