62results about How to "High extract content" patented technology

The invention relates to a preparation process of ashitaba fermented tea. The preparation process comprises the following steps: washing; spreading for airing and withering; rinsing with warm water; throwing water off; cutting; withering with hot air; rolling; fermenting; drying with gross fire; spreading for cooling; drying with complete fire; sieving and the like. In the processes of rolling and fermenting, a proper amount of exogenous enzymes is added to assist fermentation of ashitaba. According to the ashitaba fermented tea prepared by the process, the astringent taste of an ashitaba tea product is remarkably improved, and the tea brewed with hot water smells mellow and pleasant, has full taste and sweet aftertaste. Meanwhile, the main quality aspects such as the content of tea polyphenol, the content of general flavone, the content of polysaccharide and the content of free amino acids are improved.

The invention provides a white tea processing technology, which specifically includes raw material selection, withering, fermentation, impurity removal, drying and packaging. The white tea produced is of good quality, with high content of amino acids, tea polyphenols, catechins and water extracts. And the cord is flat, green and golden yellow, fresh, soup color, bright color, floral fragrance is obviously slightly sweet, rich and mellow and dry.

The invention discloses a process for extracting cordycepin (active substance) from silkworm pupa cordyceps militaris. The cordycepin is a natural nucleoside new drug extracted from Cordyceps sinensis, has the activities of inhibiting tumor, resisting plasmodium, fungus and HIV-I type virus and is capable of selectively inhibiting clostridium, thereby arousing high attention in the medicine field; the cordycepin can be artificially synthesized and also can be extracted from Cordyceps sinensis, but the yield is low, the price is high, the existing purifying and extracting method is complicated in operation, and poisonous organic solvent can cause pollution, therefore, mass production is difficult to realize. A two-aqueous-phase extraction method is adopted to extract the cordycepin from the silkworm pupa cordyceps militaris, the extract content is high, the extraction efficiency is high, labor and time are saved and the process is suitable for large-scale industrial extraction.

The invention provides instant loquat powder. The instant loquat powder is prepared by granulating the following ingredients in parts by weight: 65-80 parts of loquat flesh powder, and 2-8 parts of loquat leaf powder. The loquat flesh powder is prepared from the following steps: sieving raw materials; cleaning the sieved materials, removing kernels, and performing pulping so as to obtain pulp; adding 0.2-1% of pulp enzyme and 2-6% of beta-cyclodextrin in the pulp for enzymolysis; extracting juice, and sterilizing the juice so as to obtain clear juice; adding 0.02-0.1% of juice enzyme in the clear juice for enzymolysis and clarification; and performing concentration and pulverization so as to obtain the powdered loquat flesh powder. According to the method disclosed by the invention, the juice yield of loquat fresh is high, and the extraction ratio of effective ingredients is high; the obtained loquat flesh powder is varied in effective ingredients, high in content, few in impurities, good in solubility, and can be instantly dissolved when being brewed.

The invention provides a method and application of a photosynthetic bacteria culture solution in medicinal material planting. The method comprises the following steps: diluting the photosynthetic bacteria culture solution; and evenly irrigating soil, soaking seeds, irrigating roots and spraying. By utilizing the method, the medicinal material growth can be promoted; the occurrence of various disasters can be prevented and treated; the problem of manual culture quality reduction caused by utilizing chemical fertilizers and pesticides can be solved; the traditional Chinese medicine extract content can be increased; the content of the effective components in the traditional Chinese medicinal material is increased; the heavy metal content is reduced; the utilization of the Chinese medicinal material is improved; the sustainable utilization of the Chinese medicinal material is realized; the resources are protected; the implementation of GAP (good agriculture practice) in China can be improved; the problem that crop rotation can not be performed in Chinese medicinal material planting can be solved; and the fertilizer amount is saved by one third.

The invention creatively provides inonotus obliquus milk tea powder and a preparation method thereof. The preparation method comprises the following steps of preparing an inonotus obliquus extracting solution through water extraction three times, then mixing the following components in parts by weight of 50-80 parts of the inonotus obliquus extracting solution, 20-50 parts of skim milk, 5-10 parts of maltodextrin, 2-5 parts of a natural sweetener and 1-2 parts of carboxymethyl cellulose sodium so as to obtain a mixture, and performing homogenizing, vacuum concentration and spray drying so as to obtain instant powder namely the inonotus obliquus milk tea powder. According to the inonotus obliquus milk tea powder and the preparation method disclosed by the invention, inonotus obliquus extraction ingredients are creatively used as effective components for replacing coffee, so that the milk tea powder has the fragrance of caramel, has the mouth feel similar to the coffee, and is a healthy beverage integrating health care and leisure.

The invention discloses a preparation method of toona sinensis fermented black tea. The preparation method comprises the following steps: (1) withering; (2) rolling; (3) fermenting; (4) primarily drying through gross fire; (5) drying by final fire; (6) spreading for airing; and (7) packaging. According to the preparation method, toona sinensis leaves are adopted to be fermented to prepare tea for the first time, and are properly withered and fermented, so that the enzyme activity of the fresh leaves is activated, macromolecule nutrient substances in the fresh leaves are degraded into absorbable micromolecule substances, the content of aqueous extracts is increased, and nutrients in tea water are rich; furthermore, through sufficient fermentation, the bitter taste of the finished tea is improved, so that the tea is mellow to be easily accepted by people. Meanwhile, a conventional process of drying by final fire can be changed, and a two-time drying method is adopted, so that the toona sinensis fermented black tea has special quality. The content of functional ingredients such as total flavonoids and soluble sugar can be increased, so that the toona sinensis fermented black tea is endowed with a certain health care function.

The invention discloses an organic and inorganic compound fertilizer for radix pseudostellariae. The organic and inorganic compound fertilizer comprises an organic fertilizer and an inorganic fertilizer, wherein the inorganic fertilizer comprises urea for a nitrogen fertilizer, calcium superphosphate for a phosphate fertilizer and potassium sulphate for a potash fertilizer; the formula is prepared from the following raw materials in parts by weight: 1200-1500 parts of an organic fertilizer, 90-120 parts of urea, 60-90 parts of calcium superphosphate and 120-150 parts of potassium sulphate; the raw materials are mixed evenly to prepare the organic and inorganic compound fertilizer; and an organic cow dung fertilizer metabolized by earthworms is adopted by the organic fertilizer. The radix pseudostellariae is scientifically fertilized with the organic and inorganic compound fertilizer, so that the fibrous root of the radix pseudostellariae can be reduced; the utilization rate of the fertilizer is improved; the physical and chemical properties of the soil are improved; and the moisture content, the ash content and the extract content of the radix pseudostellariae are greatly improved, so that the high-yield and high-quality radix pseudostellariae is obtained; the production cost can be greatly reduced; the organic and inorganic compound fertilizer has the characteristics of being high in utilization rate, stable in performance, simple, feasible and free of a damp coagulation phenomenon; and large-area popularization and application are facilitated.

The invention provides a brackish water and fresh water mixed irrigation method for saline-alkali soil. The irrigation method is characterized by comprising seedling-stage irrigation, intermediate-stage irrigation and cutting-stage irrigation. According to the seedling-stage irrigation, the ratio of times of rotation irrigation of brackish water to fresh water is 1:4, and the amount of irrigation at each time is 300 to 400m<3> / hectare. According to the intermediate-stage irrigation, the ratio of times of rotation irrigation of amino acid-enriched saline water to the fresh water is 1:3, irrigation is carried out for one time every five to seven days, and the amount of irrigation at each time is 400 to 500m<3> / hectare. The amino acid-enriched saline water is formed by dissolving an amino acid fertilizer into the brackish water, and components of the amino acid fertilizer comprise 5 to 6 percent of valine, 7 to 9 percent of cystine, 3 to 4 percent of aspartic acid, 2 to 3 percent of hydroxyproline, 3 to 5 percent of tryptophan, 3 to 6 percent of serine, 7 to 8 percent of threonine and 10 to 16 percent of microelement. Amaranthus hypochondriacus is planted by using the irrigation method, the annual output of fresh grass is 20,000 to 22,000kg / acre, and the protein content of the fresh grass is 25.2 to 28.8 percent.

The invention discloses composite walnut leaf tea and a preparation method thereof. The composite walnut leaf tea is composed of 50-80 parts of walnut leaf tea, 5-30 parts of tea leaves, 2-15 parts of folium mori tea, 10-20 parts of folium cortex eucommiae tea and 1-10 parts of corn stigmas. The preparation method comprises the following steps: firstly, carrying out steps of washing, drying, leaves cutting, enzyme deactivating, rolling and baking on the front four fresh leaves; mixing the treated leaves according to the formula ratio; fermenting and airing; adding the corn stigmas and mixing uniformly; and pressing and molding to prepare the composite walnut leaf tea. According to the composite walnut leaf tea, one more choice is provided for comprehensively utilizing walnut leaves; the composite walnut leaf tea has certain curative effects on hypertension and hyperlipidemia and is health-care tea with abundant nutrition and good mouth feel.

The invention provides a preparation process flow for health-care folium mori tea and relates to the technical field of health-care tea. According to the preparation process flow, Harpin protein and an exogenous amino acid are used for adjusting the folium mori growth process so as to increase the accumulation amount of secondary metabolism products of folium mori, improve the quality of folium mori fresh leaves and improve the processing performance; the folium mori is subjected to far-ultraviolet radiation pavement and microwave green removal; a substance change dynamic condition and the sensory quality of the folium mori in the pavement and green removal process are recorded; tests are carried out on obtained high-temperature oxidase according to different free states and immobilized states; a substance change dynamic condition and a quality forming mechanism in a folium mori tea processing method can be recorded. According to the preparation process flow, the content of special amino acids of the folium mori and the content of flavone of the folium mori are increased by adopting biological technological means; the bitter taste is reduced and the content of aqueous extracts of finished products is increased by exploring and using far ultraviolet and instantaneous high-pressure technologies and the like, and the resource utilization rate of tea leaves can be increased greatly.

The invention discloses a green tea planting method, comprising the steps of land selection, land preparation, planting trench digging, tea seedling selection, transplanting, tea garden management and overwinter management. According to the green tea planting method discloses by the invention, the transplanting and planting survival rate of tea seedlings can be increased, the tea seedling survival rate and the tea bush survival rate are high, enthusiasm of tea growers is improved, afforestation cost is lowered, and the green tea planting method is beneficial for forestland management, and has a good economic benefit. In addition, the quality of finished tea products is higher.

The invention provides yellow tang sulfated polysaccharide as well as a preparation method and application thereof, and relates to the technical field of polysaccharide. The preparation method of the yellow tang sulfated polysaccharide comprises the step of carrying out edulcoration, aqueous extraction, alcohol precipitation and dialysis on yellow tang by adopting alcohol or an aqueous solution of alcohol so as to obtain a polysaccharide crude product. A polysaccharide component with large molecular weight is purified and obtained by adopting an anion-exchange column, and a gel column is adopted for purifying the polysaccharide component with large molecular weight so as to obtain the yellow tang sulfated polysaccharide. The preparation method is high in raw material utilization rate, mild in technology and high in purity, and the stability of the active structure of the sulfated polysaccharide is sufficiently guaranteed. Poisonous and harmful reagents such as acid and alkali are not used in the preparation process, and the yellow tang sulfated polysaccharide is applicable to large-scale production. Moreover, the invention also relates to the yellow tang sulfated polysaccharide prepared by the preparation method and application thereof. The sulfated polysaccharide is large in molecular weight and high in acidophobe content, can adjust the level of intestinal flora, is applied to preparation of products for adjusting intestinal functions, and has great development and application prospects.

A method for treating wood having a high extractive content is disclosed. The method generally comprises applying to the wood a coating comprising a water-based polyurethane dispersion and a crosslinker. The dispersion can be cured and one or more additional coatings can be applied. The method results in reduced migration of extractives to the surface of the wood, improved adhesion and / or reduced staining of the wood.

The invention relates to the field of tea processing, in particular to a making technology of spring tippy tea. The method comprises the procedures of picking, vacuum freezing, withering, leaf selecting, vacuum microwave fixing, spreading for cooling, rolling, deblocking, shaping and the like. According to the making technology, the traditional rolling time is shortened to 6-10 min from original 20-25 min, the spring tippy tea made in the time period is pure in taste, reduced in bitter taste and suitable for consumption of modern urban crowds, the appearance is light green in color and luster, full of fluff and more attractive, the commodity appearance aesthetic feeling is enhanced, market evaluation is good, the tea making yield per unit time is increased, and electric charge and labor charge are reduced.

The invention discloses a health care wine preparation method. The health care wine takes Chinese yam, sorghum and oat as main materials, the main materials are divided into three equal parts, auxiliary materials of lily root flour, walnut powder and lotus root flour are added, under different temperatures, multitime fermentation and filtering are carried out, the alcohol content of the health care wine is not high, and the heavy metal content in the health care wine is reduced. The prepared health care wine has the efficacy of supplementing energy and nourishing lung, has obvious curative effect for lung diseases such as chronic bronchitis, can enhance human immunity by long-term drinking, and provides vigorous energy to people.

The invention discloses triticale malt for beer brewing as well as a preparation method and application of the triticale malt, and belongs to the technical field of beer production. Through the stepsof wheat selection, wheat soaking, germination, drying, coking and de-rooting, the thousand-grain weight and protein content in the wheat-selecting stage can be accurately controlled, the temperatureand humidity of wheat soaking and germination are controlled, the full dissolution of wheat endosperm is promoted through ventilation and wheat turning in the germination stage, and the temperature and time of drying and coking are controlled to give the malt color and aroma; and the triticale malt with excellent indexes is obtained by adopting the method provided by the invention, and the triticale malt is subjected to wort preparation and beer brewing to produce characteristic triticale malt beer by controlling the saccharification process and brewing conditions to meet the market demand.

The invention relates to spleen-refreshing and child-nourishing granules, and a preparation method and quality control method thereof. The spleen-refreshing and child-nourishing granules are preparedaccording to the following method: taking sowthistle tasselflower herb, pilose gerbera herb, pittosporm roots and rhizomes of broadleaf common valeriana according to a prescription amount, adding warmwater at 40-60 DEG C for soaking for 0.5h, performing decocting twice, combining decoctions, performing filtering, concentrating the obtained filtrate to a clear paste with a relative density of 1.15-1.18 (80 DEG C), adding auxiliary materials, and performing granulation. The method can sufficiently retain active ingredients. The invention also discloses a quality control method for the spleen-refreshing and child-nourishing granules, and the method can better control the product quality.

The invention belongs to the field of codonopsis pilosula processing, and particularly relates to a fresh codonopsis pilosula drying method. The preparation method comprises the steps of putting a food drying agent into cleaned fresh codonopsis pilosula; drying for 1 to 2 hours, putting the mixture into a constant-temperature box at 40 to 45 DEG C and drying for 5 to 8 hours; taking out the codonopsis pilosula when the moisture content of the codonopsis pilosula reaches 50 to 56 percent; taking out the codonopsis pilosula, rubbing and cutting the codonopsis pilosula into sections, and placingthe codonopsis pilosula at room temperature for 3 to 7 days until the moisture content of the codonopsis pilosula is not less than 60%; placing the codonopsis pilosula back into the constant-temperature box at 60 to 65 DEG C again for drying for 7 to 9 hours; and finally taking out the codonopsis pilosula when the moisture content of the codonopsis pilosula reaches 16% to acquire dried codonopsispilosula with extract content of 55% or above. The method is simple in process, low in cost, small in pollution, excellent in dried codonopsis pilosula product efficacy, high in extract content and non-toxic in processing, is a green processing method, has a far-reaching influence on development of codonopsis pilosula industry, has a wide market prospect, and is convenient to popularize and use.

The invention provides an extraction method of a lactic acid bacteria lysing active matter and the lactic acid bacteria lysing active matter obtained by the extraction method. The extraction method of the lactic acid bacteria lysing active matter comprises the following steps: diluting lactic acid bacteria sludge to obtain bacteria liquid, and then crushing the bacteria liquid by using a high-voltage pulsed electric field to obtain the lactic acid bacteria lysing active matter. According to the method, the high-voltage pulsed electric field is adopted to crush the lactobacillus sludge obtained by fermentation, cell membrane rupture and intracellular cytoplasm outflow are finally caused by increasing cell membrane permeability and weakening cell membrane strength, and the method has the characteristics of low energy consumption, short treatment time, high efficiency and the like. The high-voltage pulse electric field technology is utilized to denature and inactivate intracellular polysaccharide clastic enzyme, and the polysaccharide decomposition capability is not realized any more, so that the extracted polysaccharide is higher in content and better in antioxidant activity.

The invention discloses a processing method of lithocarpus litseifolius dark green tea. The method includes the steps of harvesting lithocarpus litseifolius tender leaves to be thoroughly cleaned, adding cellulase and pectinase for soaking, scooping up the leaves to be drained, adding the drained lithocarpus litseifolius tender leaves to a roller fixation machine for low-temperature fixation, putting lithocarpus litseifolius leaves obtained after fixation into a tea kneading machine to be kneaded, inoculating lactic acid bacteria, acetic acid bacteria and eurotium into the kneaded lithocarpuslitseifolius leaves for pile fermentation, drying the fermented tea leaves for 6-8 hours at the temperature of 40-60 DEG C, putting the dried tea leaves in a warehouse to be naturally fermented, and conducting packaging to obtain the lithocarpus litseifolius dark green tea. The novel drink tea with a healthcare function is prepared by effectively combining the three-highs reduction medicinal valueof lithocarpus litseifolius and the unique three-highs reduction characteristic of dark green tea, the tea has the remarkable functions of reducing blood glucose, blood pressure and lipid and is novel healthcare tea which prevents and treats the cerebrovascular and cardiovascular arteriosclerosis, diabetes and the hypertension, the new product is developed for the lithocarpus litseifolius, and the benefits of the lithocarpus litseifolius are improved.

The invention provides crabapple granulated tea and a preparation method and application thereof, and belongs to the technical field of substitutional tea products. The crabapple leaves and the substitutional tea are uniformly mixed according to a certain proportion, and a framework material aqueous solution is used as an adhesive to prepare the granulated tea. The product has the advantages of smooth surface, roundness and uniform size, clear and transparent soup color, high tea water extract content, bitter taste first and sweet aftertaste, stable release of particle contents, brewing resistance, small size, exquisite package, convenience in carrying and drinking and the like; and meanwhile, the preparation process is scientific, rigorous and extremely suitable for large-scale industrialproduction, so that the beverage has good practical popularization and application value.

The invention relates to a preparation method of peony flower fiber. The preparation method comprises the following steps of (1) preparing a peony flower extract solution; (2) performing swelling treatment on cationic viscose fiber; (3) preparing peony flower extract fiber; (4) spraying soft water; (5) spraying a softening agent; and (6) performing post-treatment. According to the preparation method of the fiber, firstly, peony flower extract powder is uniformly dispersed in deionized water to prepare the peony flower extract solution, and then the peony flower fiber is prepared in a spraying mode; and in the method, no strong acid or strong base is involved in the adsorption process of the peony flower extract, and high temperature does not need to be used, so that effective components in the peony flower extract cannot be destroyed, the content of the peony flower extract in the prepared fiber is high, and the prepared composite fiber is resistant to oxidation, capable of removing DPPH free radicals and good in antibacterial function.

The invention discloses a preparation method for ultrahigh-concentration wort. The method comprises the following steps: at different temperature rising phases of a wort saccharifying process, sequentially adding a mixture of compound protease and neutral protease, and flavourzyme; after saccharifying, filtering and clarifying wort; adding hops particles with the mass percentage of 0.1%; and after boiling the wort, convoluting and depositing; and adding high malt syrup into the clarified wort and setting to 20oP. According to the method disclosed by the invention, under the condition that an existing saccharifying process of wort is not changed, the contents of extracts and amino acids of the ultrahigh-concentration wort can be improved by more than 5% and more than 50% obviously and respectively; after being fermented, beer has high fermentation degree, ethanol yield and content of flavor substances, so that an ultrahigh-concentration brewing period is shortened greatly and the quality of the ultrahigh-concentration brewed beer is improved.

The invention aims at providing a method for screening an agilawood edgeworthia chrysantha strain, and belongs to the technical field of strain screening. The method is characterized by orderly comprising the following steps: inoculating and sampling fungi, screening dominant bacteria of facilitating aquilaria sinensis edgeworthia chrysantha by three indexes such as the extract content of an edgeworthia chrysantha region, the hole dyeing area and the bacteria colonization rate according to the flow. The agilawood edgeworthia chrysantha fungi are screened by adopting the method, and the strain which is strong in infection ability, high in extract content of the edgeworthia chrysantha region and good in edgeworthia chrysantha quality can be selected. Thus, an effective bacterium screening method is provided for research of facilitation of aroma production by fungi-mediated microbiotic edgeworthia chrysantha, and is popularized and used in production.

The invention relates to a slow-release fertilizer based on traditional Chinese medicine additives and a preparation technology. The slow-release fertilizer is prepared from the following components in parts by weight: 3 to 5 parts of traditional Chinese medicine additives, 20 to 30 parts of animal bone protein, 5 to 8 parts of organic humic acid, 10 to 15 parts of multi-element slow-release fertilizer, 10 to 15 parts of plant mixture, 5 to 10 parts of crosslinking agent, and 10 to 20 parts of water. The preparation technology comprises the following steps of after the traditional Chinese medicine additives, the plant mixture, the animal bone protein, the crosslinking agent and the slow-release additives are prepared, preparing the raw materials in parts by weight for further use; uniformly mixing the raw materials, so as to obtain fertilizer particles which can be balled by hands and be dispersed after falling onto ground; composting the fertilizer particles, spreading and airing after composting is finished, granulating by a granulating technology, and packaging, so as to obtain the finished product of the slow-release fertilizer. The prepared slow-release fertilizer has the advantages that the period of delaying the release of nutritional elements is long, the nutritional elements can be continuously supplied, and the traditional Chinese medicine additives can provide rich nutritional matters and regulate the acid and alkaline balance of the soil.

The invention relates to flos sophorae tea and a method for processing the same. The method includes steps of (1), collecting fresh flos sophorae; (2), withering the fresh flos sophorae for 3-4 h until the moisture content of the flos sophorae is 70-72% so as to obtain withered flos sophorae; (3), killing enzymes in the withered flos sophorae to obtain enzyme-killed flos sophorae; (4), drying the enzyme-killed flos sophorae by the aid of microwave until the moisture content of the enzyme-killed flos sophorae is 35-40% so as to obtain primarily baked flos sophorae; (5), carrying out wrapping-twisting on the primarily baked flos sophorae for 25-30 minutes while the primarily baked flos sophorae is hot until the primarily baked flos sophorae is twisted and is approximately granular, and then baking the primarily baked flos sophorae by full fire until the moisture content of the flos sophorae is 5-7% so as to obtain the flos sophorae tea. The flos sophorae tea and the method have the advantages that cells in the flos sophorae can be appropriately broken, accordingly, the content of water extract can be increased, and the flos sophorae tea is attractive.

The present invention belongs to the technical field of tea leaf processing and specifically discloses a preparation method of old tree white tea. The preparation method of the old tree white tea comprises the following steps: (1) picking is conducted: fresh single buds of old tea trees are picked; (2) withering is conducted: the fresh single buds are sequentially subjected to a natural spreadingtreatment, a fluorescent lamp irradiation treatment, a yellow light irradiation treatment, a rolling machine pressure-free empty rolling treatment, etc.; (3) drying is conducted: tea leaves are successively subjected to an artificial shaking treatment, a vacuum secondary drying treatment and a secondary drying treatment; and (4) screening is conducted. The steps of the preparation method of the old tree white tea are combined; the preparation method effectively improves shapes, color and fragrance of the old tree white tea; and the prepared old tree green tea is high in sensory quality, high in nutritional value and good in quality.

The invention discloses a preparation method for a cyclocarya paliurus Batal. and leaf tea beverage. The preparation method comprises the following steps: (1) spraying a mixed bacteria solution of lactobacillus plantarum and saccharomycetes to radix puerariae, rhizoma polygonati, cortex cinnamomi, folium mori, aloe, folium mali hupehensis, and dried orange peel for fermentation; (2) after fixing,rolling, and sun-drying of fresh leaves of cyclocarya paliurus Batal., spraying a mixed bacteria solution of aspergillus niger and bacillus coagulans for fermentation; (3) mixing the two fermented materials in proportion, and then carry outing pile-fermentation; and (4) finally, steaming the mixed material with steamed, performing press forming, and performing drying. The tea beverage prepared bythe invention has the advantages of high juice yield, high content of nutritional active substances including tea polyphenol, general flavones and the like, and has better sensory quality and a healthcare effect.

The invention discloses a preparation method of a liquid culture medium suitable for tender stems of dendrobium officinale. The preparation method comprises the following steps of adding coconut juice, a potato extract, an apple extract and other materials into 5 to 7 times of water under room temperature, and uniformly mixing to obtain a product A; pre-dissolving 2,4-D and NAA (naphthylacetic acid) by anhydrous ethanol, and preparing into a solution with concentration of 0.5 to 1.5 mg / ml to obtain a product B; and loading the product A and the product B into a tissue culture bottle, uniformlymixing, sterilizing for 20 to 30 min by steam at high temperature, and cooling to obtain the liquid culture medium. The liquid culture medium disclosed by the invention has the beneficial effects that by adding and controlling the concentrations of the coconut juice, the apple extract and the potato extract, the contents of polysaccharide and extracts in the tender stems of the dendrobium officinale are increased.