The invention discloses a preparation method for
peptide chelated calcium of
clanis bilineata tsingtauica
larva. The preparation method comprises the following steps:
degreasing the
clanis bilineata tsingtauica
larva and then carrying out superfine crushing, adding water and uniformly mixing, and adjusting the pH value to 2-9; adding
protease in the obtained mixed liquid, stirring for
enzymatic hydrolysis, then inactivating the
protease to obtain a crude enzymatic
hydrolysate, and centrifuging to obtain an supernatant liquid; taking the obtained supernatant liquid and removing impurities to obtain a purified liquid, and carrying out separation and purification by virtue of at least one
ultrafiltration membrane with a molecular weight
cut-off of 1-50kD to obtain enzymatic hydtolysis
peptide components with different molecular weights; adding a
calcium source, and chelating to obtain a crude
peptide chelated calcium product of the
clanis bilineata tsingtauica
larva; and collecting precipitates after concentration and
precipitation, and
drying to obtain the peptide
chelated calcium product of the clanis bilineata tsingtauica larva. According to the method disclosed by the invention, a method of classifying through the
ultrafiltration membranes with different molecular weight
cut-off at first, and then chelating is adopted for the enzymatic product, on one hand, the technical
bottleneck that
a peptide calcium chelate is difficult to separate is solved; and on the other hand, the peptide chelated
calcium products with different molecular weights of the clanis bilineata tsingtauica larva are prepared and can be used by people with different needs, thus the use efficiency of the products is increased, and the pertinence of the products is improved.