The invention relates to culture solution filling method and
system for
chlamydomonas culture. The method comprises the following steps: heating, disinfecting and cooling sea water, and then storing the sea water; mixing the sea water and a culture solution with nutritive salt in a various nutritive salt tanks, and filtering through a sand filtering device; and inputting the mixture into a
chlamydomonas culture reactor through a pipeline with a quantitative control clamp. The
system comprises a constant-pressure
storage tank which is provided with a solution transmission pipeline, and is connected with a sea
water heating and disinfecting device, and a nutritive salt tank which is used for filling the nutritive solution to the solution transmission pipeline through a pump, wherein the output end of the solution transmission pipeline is connected with the
branch of a material liquid transmission pipeline on a culturing bracket through the sand filtering device, and is communicated with a hanging bag type
photobioreactor, and the material liquid transmitting pipeline is arranged on a culture support. The method disclosed by the invention can realize the automatic, sterile and unwatched line production of culture solution. The
system disclosed by the invention has a closing property, a systematic property, a quantitative property, a cleaning function and a buffering effect.