The invention discloses a production method for mannosylerythritol lipids. The production method includes the steps: inoculating a seed culture solution with activated pseudozyma aphidis DSMZ70725 for seed culture, and separating to obtain bacterial cells after culture; inoculating a
fermentation culture medium with the bacterial cells, carrying out
fermentation culture for 7-15 d at the temperature of 28-30 DEG C, to obtain a
fermentation liquid; and separating and purifying from the fermentation liquid to obtain the mannosylerythritol lipids, wherein the fermentation culture medium contains a
vegetable oil. With the
vegetable oil as a substrate and under the appropriate process, the mannosylerythritol lipids are produced through microbial
cell transformation. The substrate used in the method is a
renewable resource, the product is non-toxic and
environmentally friendly, and the obtained fermentation liquid has
high surface activity; in the fermentation process,
mycelium clusters are small, the substrate is transformed fully, and the crude product yield is greatly improved; and the production process is safe, simple and convenient, and easy to operate, the product quality is high, and industrialized production is easy to realize.