Cosmetic pigments, their production method, and cosmetics containing the cosmetic pigments
A technology of cosmetics and pigments, applied in the direction of cosmetic preparations, cosmetics, cosmetics, etc., can solve the problems of poor skin adhesion, no moisturizing feeling, poor affinity, etc., and achieve excellent use feeling, excellent skin affinity, and safety sex high effect
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manufacture Embodiment 1
[0069] (Manufacturing Example 1: Manufacturing Examples of MEL-A, MEL-B, and MEL-C)
[0070] One inoculation loop of Pseudozyma antarctica NBRC 10736 (obtained from: NITE Biological Resource Center) flora was inoculated into a seed medium (20 ml / 500 ml shaking flask) to perform seed culture. Incubate overnight at 30°C. The obtained culture solution was used as an inoculum. The composition of the seed medium is: 4W / V% glucose, 0.3W / V% NaNO 3 , 0.02W / V%MgSO 4 ·7H 2 O, 0.02W / V% KH 2 PO 4 , 0.1W / V% yeast extract. For cultivation, 75 ml of the above inoculum was inoculated into 1.5 L of production medium (5 L tank), and cultured in a 5 L tank at 30° C., 300 rpm (stirring rotation), and 0.5 L / min (Air). The composition of the production medium is: 3W / V% soybean oil, 0.02W / V% MgSO 4 ·7H 2 O, 0.02W / V% KH 2 PO 4 , 0.1W / V% yeast extract. 250 ml of the culture solution was centrifuged (6500 rpm, 30 min), the supernatant was removed, and the precipitate (bacteria) was recovere...
manufacture Embodiment 2
[0072] (Manufacturing Example 2: Manufacturing Example of Inverted MEL-B)
[0073]Inoculate 0.2ml of Pseudozyma tsukubaensis (シュードザイマッックババンシス) frozen stocks into 20ml of YM medium (1W / V% glucose, 0.3W / V% yeast extract, 0.5W / V% polypeptone, 0.3W / V (V% malt extract, pH 5.6) / 500ml capacity shaking flask, cultured overnight at 26°C, 180rpm, as seed bacteria. 0.2 ml of seed bacteria were inoculated again into 20 ml of YM seed medium / 500 ml capacity shaking flask, and cultured overnight at 26° C. and 180 rpm to obtain seed bacteria. Inoculate 20 ml of inoculum into 2 L of YM medium / 5 L tank containing 15 W / V% olive oil, and cultivate for 8 days at 26° C., 300 rpm (1 / 4 VVM, 0.5 L air / min). The culture solution was centrifuged at 7,900rpm·60min·4°C, and separated into bacteria (including MEL-B) and supernatant. Add 80ml of ethyl acetate to the cell components, stir up and down to fully suspend the cells, and then perform centrifugation at 7,900rpm·30min·4°C. An equal amount of satu...
manufacture Embodiment 3
[0075] (Manufacturing Example 3: Manufacturing Example of MEL-C)
[0076] Inoculate 0.2 ml of the frozen stock of Pseudozyma hubeiensis into 20 ml of YM seed medium / 500 ml of shaking flask, and cultivate it overnight at 26° C.·180 rpm to serve as seed bacteria. 0.2 ml of seed bacteria was re-inoculated into 20 ml of YM seed medium / 500 ml capacity shaker flask, and cultured overnight at 26° C.·180 rpm to serve as seed bacteria. Inoculate 20ml of inoculum into 2L of YM medium / 5L·tank and culture at 26°C·300rpm (1 / 4VVM, 0.5L·air / min) for 8 days. The culture solution was centrifuged at 7,900rpm·60min·4°C, and separated into bacteria (including MEL-C) and supernatant. Add 80ml of ethyl acetate to the cell components, stir up and down to fully suspend the cells, and then perform centrifugation at 7,900rpm·30min·4°C. An equal amount of saturated brine was added to the obtained supernatant, followed by stirring to obtain an ethyl acetate layer. An appropriate amount of anhydrous so...
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