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Production method for mannosylerythritol lipids

A production method, sugar erythritol technology, applied in the production field of mannose erythritol lipid, can solve the problems of low product yield, immature process of mannose erythritol lipid, high raw material cost, etc., and achieve high surface activity , easy operation, high product quality effect

Inactive Publication Date: 2014-02-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a production method of mannose erythritol lipid, which solves the problems of immature technology for preparing mannose erythritol lipid by the existing biological method, high raw material cost and low product yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The vegetable oil used in this example is soybean oil.

[0030] (1) Strain activation: Use sterilized maltose agar plate as the activation medium, insert the refrigerated strain Pseudozyma aphidis DSMZ70725, activate and cultivate at 28°C for 4 days, and wait until the mycelium is full Tablet spare.

[0031] (2) Seed culture: Pick 3 pieces of 1cm×1cm mycelium from the plate culture medium and put them into a 250mL Erlenmeyer flask containing 50mL seed culture solution, at 28°C and 220rpm for 4 days; The liquid was centrifuged, the bacterial cells were taken, and washed 3 times with 0.9% sodium chloride solution;

[0032] Wherein, the components of the seed culture solution include 4.0% of glucose, 0.3% of sodium nitrate, 0.03% of magnesium sulfate, 0.03% of potassium dihydrogen phosphate, 0.1% of yeast powder, and distilled water.

[0033] (3) Fermentation culture: the bacterial cells were inserted into a 500mL Erlenmeyer flask filled with 100mL fermentation medium at...

Embodiment 2

[0038] The vegetable oil used in this example is blended oil, calculated according to the volume ratio, the composition is soybean oil: peanut oil: rapeseed oil = 6:3:1.

[0039] (1) Strain activation: Use sterilized maltose agar plate as the activation medium, insert the refrigerated strain Pseudozyma aphidis DSMZ70725, activate and cultivate at 28°C for 4 days, and wait until the mycelium is full Tablet spare.

[0040] (2) Seed culture: Pick 3 pieces of 1cm×1cm mycelium from the plate culture medium and put them into a 250mL Erlenmeyer flask containing 50mL seed culture solution, at 28°C and 220rpm for 4 days; The liquid was centrifuged, the bacterial cells were taken, and washed 3 times with 0.9% sodium chloride solution;

[0041] Wherein, the components of the seed culture solution include 4.0% of glucose, 0.3% of sodium nitrate, 0.03% of magnesium sulfate, 0.03% of potassium dihydrogen phosphate, 0.1% of yeast powder, and distilled water.

[0042] (3) Fermentation cultu...

Embodiment 3

[0047] The vegetable oil used in this example is soybean oil.

[0048] (1) Strain activation: Use sterilized maltose agar plate as the activation medium, insert the refrigerated strain Pseudozyma aphidis DSMZ70725, activate and cultivate at 28°C for 4 days, and wait until the mycelium is full Tablet spare.

[0049] (2) Seed culture: Pick 3 pieces of 1cm×1cm mycelium from the plate culture medium and put them into a 250mL Erlenmeyer flask containing 30mL seed culture solution, at 28°C and 220rpm for 3 days; The liquid was centrifuged, the bacterial cells were taken, and washed 3 times with 0.9% sodium chloride solution;

[0050] Wherein, the components of the seed culture solution include 4.0% of glucose, 0.2% of sodium nitrate, 0.02% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate, 0.1% of yeast powder, and distilled water.

[0051] (3) Fermentation culture: the bacterial cells were inserted into a 500mL Erlenmeyer flask filled with 100mL fermentation medium at...

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Abstract

The invention discloses a production method for mannosylerythritol lipids. The production method includes the steps: inoculating a seed culture solution with activated pseudozyma aphidis DSMZ70725 for seed culture, and separating to obtain bacterial cells after culture; inoculating a fermentation culture medium with the bacterial cells, carrying out fermentation culture for 7-15 d at the temperature of 28-30 DEG C, to obtain a fermentation liquid; and separating and purifying from the fermentation liquid to obtain the mannosylerythritol lipids, wherein the fermentation culture medium contains a vegetable oil. With the vegetable oil as a substrate and under the appropriate process, the mannosylerythritol lipids are produced through microbial cell transformation. The substrate used in the method is a renewable resource, the product is non-toxic and environmentally friendly, and the obtained fermentation liquid has high surface activity; in the fermentation process, mycelium clusters are small, the substrate is transformed fully, and the crude product yield is greatly improved; and the production process is safe, simple and convenient, and easy to operate, the product quality is high, and industrialized production is easy to realize.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a production method of mannose erythritol lipid. Background technique [0002] Surfactants are amphiphilic substances that can significantly reduce surface tension and play an important role in industrial and agricultural production. However, most of the surfactants used in the domestic market are artificially chemically synthesized from petroleum, which is likely to cause problems such as environmental pollution and quality safety in the production process. Surfactants obtained by microbial transformation, namely biosurface Active agents do not have this problem. Therefore, biosurfactants will become one of the options to replace chemically synthesized surfactants. Biosurfactant is a substance with high surface activity secreted by microorganisms in the metabolic process. It has the characteristics of biocompatibility, biodegradability, non-toxic or low toxicity, and also...

Claims

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Application Information

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IPC IPC(8): C12P19/44C12R1/645
Inventor 陈启和范琳琳董亚晨
Owner ZHEJIANG UNIV
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