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Method for purifying hydrophilic mannosylerythritol lipids

A purification method, sugar erythrose technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of high cost, inability to meet the industrial production of MELs, waste of products and organic solvents, etc., and achieve efficient and rapid separation Effect of purification, promotion of industrial production and application

Pending Publication Date: 2022-01-28
NANJING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purification method of MELs is mainly column chromatography technology, but column chromatography separation and purification technology is often costly, resulting in a large waste of products and organic solvents, which cannot meet the needs of industrial production of MELs

Method used

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  • Method for purifying hydrophilic mannosylerythritol lipids
  • Method for purifying hydrophilic mannosylerythritol lipids
  • Method for purifying hydrophilic mannosylerythritol lipids

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Take 2.5g of paste-like MELs crude product, dissolve it in 30ml of methanol, add 50-400μL of concentrated sulfuric acid until the volume fraction of sulfuric acid is 0.167-1.33%, react the above mixture at 40-60°C for 30-120min, pass TLC Observe the structural changes of products and impurities. The function of the concentrated sulfuric acid is as a catalyst in methyl esterification, and as H in the structural change of MELs + Provider, the amount of acid added will mainly affect the reaction time.

[0026] The rate of this reaction depends on the temperature and the amount of acid added. As shown in Table 1 below, the free fatty acid methyl esterification reaction is relatively fast, and the reaction can be completed within 10-30 minutes under the condition of concentrated sulfuric acid as a catalyst, and the conversion rate is close to 100%. However, it takes a long time to obtain hydrophilic MELs through this reaction. With the increase of the amount of acid added...

Embodiment 2

[0030] Take 2.5g of paste-like crude MELs product, dissolve it in 30ml of methanol, add 50μL (0.167%) concentrated sulfuric acid until the volume fraction of sulfuric acid is 0.167%, and react the above mixture at 50°C for 30min. Add 2 g of CaCO to methanol after the reaction 3 , adjust the pH to 6-7, and remove the solids by filtration. Add methanol to the obtained neutral solution, or concentrate by evaporation to adjust the concentration of MELs to 40-300g / L.

[0031] Divide the methanol solution of mannose erythritol lipid into three parts and add different proportions of n-hexane respectively. After the concentration adjustment, the volume ratio of MELs methanol solution to n-hexane is 1:1, 1:2, 1:3 respectively .

[0032] Thoroughly oscillate and mix on a spinner, then centrifuge at 12,000 rpm for 5 min, and discard the upper n-hexane phase. Then the above extraction process was repeated 3 times with n-hexane, the lower layer (methanol phase) was collected, and washed...

Embodiment 3

[0035] Take 2.5g of paste-like crude MELs product, dissolve it in 30ml of methanol, add 400μL of concentrated sulfuric acid until the volume fraction of sulfuric acid is 1.33%, and react the mixture at 50°C for 120min. After the reaction, three times the volume of pure water was added to the methanol phase, shaken evenly, and then centrifuged at 8000 rpm for 5 minutes to remove the upper liquid phase and collect the hydrophilic MELs neutral precipitate in the lower layer. In the method of the present invention, the pH adjustment method is to adjust the MELs solute, so that the solubility of MELs decreases and the H + Transfer to liquid and remove together.

[0036]Add methanol to the resulting precipitate and dilute to different concentrations (40-300g / L). Add n-hexane to the MELs methanol solution after concentration adjustment for extraction, the volume ratio of the methanol solution of the mannose erythritol lipid to n-hexane is 1:1-3, fully oscillating and mixing on the s...

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Abstract

The invention discloses a method for purifying hydrophilic mannosylerythritol lipids (MELs). The hydrophilic MELs are prepared by performing acid hydrolysis on an MELs mixture obtained by fermentation in a methanol solution, meanwhile, free fatty acid as a main impurity in the hydrophilic MELs generates fatty acid methyl ester which can be removed through normal hexane extraction, and finally the high-purity hydrophilic MELs are obtained. The MELs is a glycolipid biosurfactant which is mainly produced by fermentation of saccharomycetes. According to the method provided by the invention, rapid preparation of hydrophilic MELs can be realized, free fatty acid in crude MELs can be efficiently removed, efficient and rapid separation and purification of the product can be realized, a new scheme is provided for purification of conventional structures of MELs, and industrial production and application of MELs can be promoted.

Description

technical field [0001] The invention relates to a new method for separation and purification of glycolipid biosurfactants, in particular to a method for purification of hydrophilic mannose erythritol lipids (mannosylerythritol lipid, MELs). Background technique [0002] Surfactant is a compound with amphiphilic structure, which can significantly reduce the surface tension of liquid at low concentration. It has been widely used in many fields such as petroleum industry, environmental engineering, food industry, etc., and is known as "industrial monosodium glutamate". However, most of the current surfactants are chemically synthesized from petroleum, which will bring a series of environmental problems during their production and use. Biosurfactants are a class of surface-active amphiphilic compounds mainly produced by microbial fermentation. Compared with chemical surfactants, they have higher structural diversity, foaming properties, environmental friendliness and unique biol...

Claims

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Application Information

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IPC IPC(8): C07H15/04C07H1/06
CPCC07H15/04C07H1/06
Inventor 龙旭伟杨骐宁沈亮
Owner NANJING UNIV OF SCI & TECH
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