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Separation and purification method of mannosylerythritol lipids (MELs)

A technology of separation and purification, erythromycin sugar, applied in the field of separation and purification, to achieve the effect of high product recovery rate and purity, efficient separation and purification, and low solvent toxicity

Active Publication Date: 2018-10-09
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the deficiencies and defects of existing MELs separation and purification methods, and proposes a separation and purification method of MELs

Method used

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  • Separation and purification method of mannosylerythritol lipids (MELs)
  • Separation and purification method of mannosylerythritol lipids (MELs)
  • Separation and purification method of mannosylerythritol lipids (MELs)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take the fermentation broth rich in MELs, filter it with filter paper, and dissolve the semi-solid filter residue with enough methanol to obtain a methanol solution of MELs. Then according to the ratio of methanol: water: n-hexane=4:1:1, water and n-hexane of corresponding volume are added thereto, and the pH of the control water phase is 2, 5, 7, 9, 11, fully Mix for 2 minutes, then centrifuge at 4000 rpm for 2 minutes, and remove the lower methanol phase. The results are shown in Table 1 below. This method can achieve the purpose of separating and purifying MELs, and the acidic pH of the aqueous phase is better.

[0033] Table 1

[0034] pH of aqueous phase

Embodiment 2

[0036] Take the fermentation broth rich in MELs, filter it with filter paper, and dissolve the semi-solid filter residue with enough methanol to obtain a methanol solution of MELs. According to the ratio of methanol: water: n-hexane=2:1:1, 3:1:1, 4:1:1, 5:1:1, water and n-hexane of corresponding volume are added thereto, and the pH of the water phase is adjusted to 2. Mix well for 2 minutes on the suspension apparatus, then centrifuge at 4000rpm for 2 minutes, and take out the lower methanol phase. The results are shown in Table 2 below. This method can achieve the purpose of separating MELs.

[0037] Table 2

[0038]

Embodiment 3

[0040] Take the fermentation broth rich in MELs, filter it with filter paper, and dissolve the semi-solid filter residue with enough methanol to obtain a methanol solution of MELs. According to the ratio of methanol: water: n-hexane=2:1:0.5, 2:1:1.5, 2:1:2, 2:1:2.5, water and n-hexane of corresponding volume are added thereto, and the pH of the water phase is adjusted to 2. Mix well for 2 minutes on the suspension apparatus, then centrifuge at 4000rpm for 2 minutes, and take out the lower methanol phase. The results are shown in Table 3 below. This method can achieve the purpose of separating MELs.

[0041] table 3

[0042]

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Abstract

The invention discloses a separation and purification method of mannosylerythritol lipids (MELs). The method is based on the difference in polarity of each component in crude MELs produced by fermentation, and utilizes a methanol-water-n-hexane ternary system to perform separation and purification on the MELs. The MELs are green bio-surfactants which are secondary metabolites produced by fermentation of Pseudozyma aphidis or Ustilago aphidis. The methanol-water-n-hexane ternary extraction system provided by the invention can effectively remove impurities such as grease and free fatty acids inthe crude MELs so as to realize efficient and quick separation and purification of the MELs, and the operation process is simple and efficient.

Description

technical field [0001] The invention relates to a new separation and purification method, in particular to a new separation and purification method for mannose erythritol lipids (MELs). Background technique [0002] Surfactants are called "industrial monosodium glutamate" and are widely used in industry and our daily life. But at present, most of them are petroleum-based chemical surfactants. A large amount of chemical surfactants will have adverse effects on soil, water and organisms, and cause great harm to the environment and human health. Biosurfactants are surfactants produced by microbial fermentation. They have the advantages of low toxicity, degradability, ecological safety and high surface activity, and can replace chemical surfactants in many fields. Mannose erythritol lipids (MELs) are a class of biosurfactants that are fermented by Pseudozyma aphidis or Ustilago aphidis using oil and sugar as carbon sources. One of the most potential biosurfactants. MELs have ...

Claims

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Application Information

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IPC IPC(8): B01F17/56C07H15/04C07H1/06C09K23/56
CPCC07H1/06C07H15/04C09K23/00
Inventor 龙旭伟沈亮金明杰马科锋朱孟婕
Owner NANJING UNIV OF SCI & TECH
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