A catalyst coated membrane (CCM) comprising an anode catalyst layer having a first catalyst layer composed of a non-supported catalyst and a second catalyst layer composed of a supported catalyst, a cathode catalyst layer composed of a supported catalyst, and an electrolyte membrane interposed between the anode catalyst layer and a cathode catalyst layer, the first catalyst layer of the anode catalyst layer being disposed adjacent to the electrolyte membrane; a membrane electrode assembly (MEA) comprising the catalyst coated membrane; a method of preparing the membrane electrode assembly; and a fuel cell comprising the membrane electrode assembly, are provided. The CCM, which comprises a bilayered anode catalyst layer including the first catalyst layer composed of a non-supported catalyst and the second catalyst layer composed of a supported catalyst, exhibits reduced electrical resistance and interfacial resistance, and has increased catalyst availability. The use of the CCM and an MEA having the same results in a decrease in the interfacial resistance between the electrodes and the electrolyte membrane, a decrease in the amount of the catalyst used in the electrode catalyst layer, and a decrease in the thickness deviation in the electrode layers. The fuel cell employing the MEA exhibits maximal activity of the supported catalyst, and has improved cell characteristics such as output voltage, output density, efficiency, and the like.