55 results about "High amylase" patented technology

The invention relates to a preparation method of corn resistant starch, which uses common corn starch as raw material to produce corn resistant starch with multiple physiological functions and characteristics by combined treatment of microbial fermentation and hot pressing and cooling. The resistant starch has physiologic functions of effectively controlling body weight, preventing constipation, appendicitis, hemorrhoids and colon cancer, controlling diabetes, increasing lipid discharge, improving lipid composition, promoting absorption of zinc, calcium and magnesium ions, increasing removal of cholesterol and cholic acid, and reducing cholesterol biosynthesis, lipid absorption and fatty acid biosynthesis. The resistant starch used in food processing industry has better physicochemical properties than those of dietary fiber, low water retention capacity, good mouthfeel, no influence on food, and improve food texture. The invention solves the problems in production of resistant starch, including low yield and lack of high amylase starch in China.

The invention discloses a no-alum potato starch product containing a compound additive and a preparation method thereof, belonging to the technical field of food processing. The no-alum potato starch product comprises the following materials: potato starch, chestnut starch, high-amylase corn starch and the compound additive, wherein the compound additive comprises tara gum, abelmoschus manihot gum, salt, potassium dihydrogen phosphate and calcium chloride. The preparation method comprises the following steps of degradation of amylopectin, size mixing, curing and three-section drying and the like. The no-alum potato starch product and the preparation method disclosed by the invention have the advantages that the used composite additive is green and safe, low in cost, convenient to use and good in use effect, and is an ideal alum substitute for processing a potato starch product. The processed potato starch product is excellent in quality, has health-care action, and is good in taste, high in transparency, good in toughness and elasticity, and low in strip breaking rate, consomme rate and dissolution rate.

The invention discloses a method for biological strengthening of vinegar by using marine-derived bacillus licheniformis, and belongs to the technical field of food brewing. According to the method, bacillus licheniformis (B. licheniformis) with CCTCC NO: M 2017209 is selected from marine environment, and the B. licheniformis can grow at 65 DEG C and has a high amylase production capacity. According to the method, the B. licheniformis is made into B. licheniformis powder for enhancing fermentation of acetic acid of the vinegar, and the method has the advantages of improving the utilization ratio of raw materials, improving the product flavor of the vinegar and improving the quality of the vinegar.

The invention discloses a thermostable amylase mutant and a preparation method and application thereof, belonging to the field of genetic engineering. Bacillus stearothermophilus amylase is taken as a female parent, the N-terminal area of the bacillus stearothermophilus amylase and oligopeptide are connected through PT-linker so as to obtain the mutant, and the amino acid sequence of the oligopeptide is shown as SEQIDNO.1. Under the transformation condition, the bacillus stearothermophilus amylase is improved to be 7h from 4h of a comparison example (before mutation) in half-life period at 95DEG C. The method remarkably improves the thermostability of the amylase and provides a foundation for industrial production of the amylase.

The invention discloses a polyvinyl alcohol / high amylose biodegradable material and a melting preparation method thereof. The method comprises the following steps: uniformly mixing polyvinyl alcohol, high amylase, heat stabilizer, inorganic filler, low molecular weight plasticizer and processing assistant agent in a high-speed mixer at the temperature of 70 to 120 DEG C; and then, melting, extruding and pelleting the mixture in a double-screw extruder, wherein the temperature of a charging barrel is 130 to 190 DEG C. By the method, a composite system of the low molecular weight plasticizer is rationally selected and a proper amount of processing assistant agent and heat stabilizer are added to successfully implement the thermoplastic molding of the polyvinyl alcohol / high amylose, therefore, not only the melting processing problem of the polyvinyl alcohol / high amylase is solved, but also the processability, mechanical property and biodegradability of the composite material are improved.

The invention provides an alpha-amylase truncated body and application thereof, and relates to meaningful alpha-amylase variant which simultaneously improves thermal endurance and catalytic activity, which is characterized in that 45-467 amino acid peptide fragment of bacillus subtilis Cn7alpha-amylase is cut off, and a fragment of artificially synthetic peptide is fused on C terminal. Obtained variant alpha-amylases respectively have temperature toleration increased by 2.7 DEG C, catalytic activity increased by 2.23 times compared with the parent alpha-amylase; and the variant alpha-amylases are more beneficial to industrialized production in the starch industry than the parent alpha-amylase.

The invention discloses a preparation method of a starch-C18 fatty acid compound.The method includes the steps that high amylase corn starch and C18 fatty acid, namely, stearic acid or oleic acid or soya oil acid or alpha-linolenic acid, serve as the raw materials, and through the purification of starch, the starch-C18 fatty acid compound is formed, purified and prepared.The method has the advantages that the defect that fatty acid is prone to oxidation is overcome, the enzyme resistance of starch is improved, and the compound yield can reach about 70% at most, and high application prospects are achieved on the aspects of food production and processing.

The invention discloses a method of reducing the content of amylase in hybrid rice and belongs to the technical field of rice genetic breeding. In the method, an original hybrid rice combination: one of a parent of a non-glutinous sterile line / a non-glutinous restorer is modified into a glutinous parent. The hybrid rice is prepared through a hybridization method of a glutinous sterile line / non-glutinous restorer or a non-glutinous sterile line / a glutinous restorer to obtain a hybrid rice variety in which the content of the amylase is lower than that of the original hybrid rice. In the invention, by means of a genetic effect and a genetic dosage effect of a glutinous gene wx, a near-isogenic line combination is reduced in the content of the amylase. In addition, a growth period, a plant and leaf form, panicle traits, floral organ traits, yield traits, disease resistant performance, adaptability and the like of the near-isogenic line combination are free of significant different when the near-isogenic line combination is compared with the original hybrid rice combination. Through quality analysis and taste evaluation, a new hybrid rice variety being appropriate in the content of the amylase and having the taste and the quality which are suitable for people in different region, thereby achieving an object of genetically improving a poor taste and a poor quality due to a high amylase content in the hybrid rice in the prior art.

The invention discloses saccharomycopsis fibuligera for preparing natural aroma fermented rice filtrate and application of the saccharomycopsis fibuligera. The saccharomycopsis fibuligera CGMCC No: 21316 is preserved in the China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No: 21316. The strain has relatively high amylase and protease activity, and shows relatively high tyrosinase inhibitory activity during secondary screening. When the strain is used for fermenting rice, the variety of aroma compounds in the obtained fermented rice filtrate is increased, the content of the aroma compounds is increased, the flower and fruit aroma of the fermented rice filtrate is obviously enhanced, and the aroma of the fermented rice filtrate is richer and more pleasant.

The invention discloses a prepacked ready-to-eat fish ball and a processing method thereof. The prepacked ready-to-eat fish ball comprises the following active ingredients in percentage by weight: 65.0-75.0% of minced fillet, 0.2-0.3% of composite phosphate, 0.5-0.8% of edible salt, 1.5-2.0% of white granulated sugar, 0.5-0.7% of aginomoto, 0.15% of xylose, 0.2% of glucose, 0.5-1.5% of curdlan gum powder, 2.0-6.0% of high amylase corn starch, 3.0-9.0% of MTG modified wheat gluten, 5.0-15.0% of water, and no more than 6% of auxiliaries, spices, seasonings and edible essences; the processing method comprises the following steps: pretreating, performing empty cutting, salt cutting and mixed cutting, forming, gelatinizing, curing, oil frying, cooling, packing, performing two-stage sterilization, and cooling, thus obtaining the prepacked ready-to-eat fish ball. According to the prepacked ready-to-eat fish ball and the processing method thereof, the phenomena that the tissues of the traditional fish ball are seriously destroyed during high-temperature frying and high-temperature sterilization procedures to further cause the poorer mouth feeling can be solved.

The invention discloses a method for inducing an embryonic stem cell into pancreatic tissue-like cells. The method comprises the following steps of: using a hanging-drop preparation method to differentiate an embryonic stem cell into three germ layer cells and using a immunomagnetic bead cell sorting method to purify finalized inner germ layer cells; further inducing the finalized inner germ layer cells so as to differentiate the cells into pancreatic precursor cells; and further differentiating the pancreatic precursor cells outside the body so as to form pancreatic tissue-like cells with high amylase secretion capability. The technical scheme of the invention adopts a plurality of induction factors for induced differentiation in different stages, which proves that the embryonic stem cell has the potentiality of being differentiated into pancreatic exocrine cells, and an effective way for inducting the embryonic stem cell into pancreatic tissue-like cells is found out, so that the pancreatic precursor cells are possible to recover injury of pancreas, and more cell sources are provided for recovering pancreas.

The invention provides a high-efficiency flavor-enhancing strain and application thereof in improving the tobacco quality. The high-efficiency flavor-enhancing strain is Bacillus sp. J3 with the preservation number of CCTCC NO: M 2018707. The strain is first separated from the surface of Zimbabwe flue-cured tobacco leaves and found to have high amylase, catalase and protease activities. The wholegenome sequencing finds that the strain contains multiple sets of oxidative stress systems, high activity amylase expression systems and multiple protease gene information. In addition, the strain also has a polyglutamic acid synthesis gene cluster, and can synthesize polyglutamic acid in a large amount extracellularly. On one hand, the Bacillus sp. can shorten the time of artificially aging of the flue-cured tobacco and improve the aroma of the flue-cured tobacco and smoking quality; on the other hand, the strain can be used as a fertilizer synergist for tobacco planting to improve the planting quality of the tobacco from the tobacco planting.

The invention discloses a general bacillus subtillis combination expression vector for improving the expression level of alpha-amylase. The invention relates to the general bacillus subtillis combination expression vector including the following parts: a bacillus subtillis promoter sequence, a multiple cloning site sequence for combining components possibly related to expression regulation, and an escherichia coli copying initiation sequence. The vector can be stably copied in escherichia coli and can be combined with bacillus subtillis chromosomes for stable expressing. After the vector is combined with bacillus subtillis chromosomes, the expression level of alpha-amylase in bacillus subtillis is obviously improved.

The invention discloses an oxidation-resistant amylase mutant and a preparation method thereof, and belongs to the field of genetic engineering. The preparation method of the invention adopts Bacillus alcalophilus JN21 (CCTCC NO: M 2011231) amylase as a female parent, and performs site-directed mutagenesis of the Bacillus alcalophilus amylase sequence by molecular biology technology. Under the reconstruction conditions, the Bacillus alcalophilus amylase is treated at 40 DEG C in 500 mM H2O2 environment for 30 min, and the enzymatic activity residue is increased from 18% of a control (before mutation) case to 92%; with the strategy, the oxidation resistance of the amylase is greatly increased, which lays a foundation for the application of the amylase in industrial production fields of weaving desizing, washing agent additives, and the like. The strategy has important guidance meaning for the reconstruction of oxidation resistance of amylase and other enzymes.

The invention discloses a breeding method of rice noodles dedicated special rice, and belongs to the technical field of special rice breeding. The method comprises the following steps: selecting rice variety which is large in popularization area in production, high in yield and good in quality as a recurrent parent, using the rice variety with premature senility resistance, endurable storage, high iron and high amylase as a target trait to provide the parent, hybridizing, multiple-crossing and back-crossing introducing and verifying and screening the target traits, breeding the special rice variety which has similar agronomic trait and yield with the recurrent parent, and has high amylose content, high iron, premature senility resistance, and endurable storage and is suitable for processing nutrient rice noodles.

The invention discloses a higher-heat-stability amylase mutant, and a preparation method and application thereof, belonging to the field of enzyme engineering. A molecular biology technique is utilized to perform multiple site-directed mutagenesis to obtain the higher-heat-stability amylase mutant. The half life of the obtained amylase at 60 DEG C is enhanced to 23.9 minutes from 3.2 minutes of the control sample (before mutation). The scheme can obviously enhance the heat stability of the amylase, and provides basis for industrial production. The scheme has important instruction meanings for property modification of other enzymes.

The invention provides a high-specific-activity high-catalytic-efficiency amylase mutant and a preparation method and application thereof and relates to the field of gene engineering. Acid amylase derived from Talaromyces leycettanus JCM12802 is used as a female parent, and the wild type is subjected to site-directed mutagenesis by means of molecular biological techniques. Under the modification condition, the amylase mutant has significantly higher catalytic efficiency and specific activity than the wild (before mutation) female parent. By using the scheme, it is possible to greatly increase the specific activity and acting conditions of amylase, and basis is provided for the amylase in industrial production field. The scheme is of important guidance for increasing the catalytic efficiency and specific activity of amylase and other enzymes.

The invention discloses a method for modifying corn starch with different amylose contents by air atmospheric pressure plasma jet. The method comprises preparing waxy corn starch, common corn starch and high amylase corn starch into starch emulsions having a mass concentration of 5-15%, directly treating the starch emulsions with an air atmospheric pressure plasma jet device for 1-7 minutes, centrifuging the starch emulsions to obtain precipitates, washing the precipitates for 3 times with absolute ethanol, and performing vacuum freeze drying to obtain modified starch. The method uses the airatmospheric pressure plasma jet technology to treat the three corn starch emulsions, and introduces some oxygen-containing polar groups into the corn starch for hydrophilicity improvement, mainly reflected in increase of water bonding ability and water expansion volumes, of the starch so as to further improve the practical application performance of the three kinds of corn starch. The method can also provide a new direction for starch modification for scholars who study the direction in the future, and provides more theoretical supports for the starch research field. The modified starch produced by the method can also be better applied to convenience food.

A liquid cleaning agent containing: a) 20 to 70 wt % water, b) at least one amylase preparation, c) at least one Ca2+-ion source, d) lactic acid or a lactic acid salt, is notable for elevated amylase stability and improved cleaning performance.

The invention discloses an amylase mutant with improved heat stability and application thereof and belongs to the field of genetic engineering. The amylase mutant uses Bacillus alcalophilus JN21 (CCTCC NO:M 2011231) amylase as a female parent, and a molecular biological technique is adopted to perform site-specific mutagenesis to a Bacillus alcalophilus amylase sequence. Under the transformation condition, the Bacillus alcalophilus amylase is improved from 15.3min (before mutation) to 43.8min at a 50-DEG C half-life period. By means of the application, the heat stability of the amylase can be improved remarkably, and a foundation is provided for industrial production of the amylase. The application has important guiding significance on modification of other enzymes.

The invention relates to a screening method for mutated strains capable of realizing high yield of amylase. The method comprises a step of screening of mutated strains, i.e., screening of first-generation strains having undergone mutation; the step comprises a substep of primary screening and a substep of secondary screening; the secondary screening is carried out in a manner the same as the manner used in primary screening so as to obtain second-generation mutated starting strains; then mutation and screening are carried out again; and the screening method for the generation II and generation III to generation n is same, and mutation and screening are carried out until high-amylase-yield stains meeting requirements are bred. With the screening method, the starting strains having undergone mutation treatment undergoes primary screening and secondary screening again so as to obtain the second-generation starting strains; the screening method is simple and easily practicable, and can obtain the high-amylase-yield stains in a short time; the whole screening process is low in investment and high in targeting performance; and orientated production of the amylase strains with high enzyme activity can be realized, and the method has large-scale promotion value.

The present invention discloses a method for relieving structure collapse of steamed buns by using a sour dough fermentation technology and belongs to the technical field of food processing. The method comprises steps of sour dough fermentation, mixed dough preparation, final fermenting, steaming, etc. The sour dough fermentation technology is used to effectively relieve internal shrinkage and collapse phenomena of fermented dough food caused by high amylase activity of wheat flour by inhibiting the amylase activity in the wheat flour. The method can effectively improve the physical property quality of the product, is also good in batch stability, high in safety, free of chemical addition, green and natural, and achieves an effect of obviously improving the physical property quality characteristics of the fermented dough food.

The invention provides a high-specific-activity amylase mutant with good ability to degrade raw starch and a preparation method and application thereof, and relates to the field of gene engineering. Acid amylase derived from Talaromyces leycettanus JCM12802 is used as a female parent, and the wild type is subjected to site-directed mutagenesis by means of molecular biological techniques. Under the modification condition, the amylase mutant has significantly higher specific activity than the wild (before mutation) female parent. By using the scheme, it is possible to greatly increase the specific activity and acting conditions of amylase, and basis is provided for the amylase in industrial production field. The scheme is of important guidance for increasing the catalytic efficiency and specific activity of amylase and other enzymes.

The invention provides preparation of an amylase-rich compound enzyme as well as a strain and application of the compound enzyme. A cultured fermentation product contains amylase, neutral proteases, xylanases, cellulases, glucanases and pectinases. Aspergillus oryzae is named as aspergillus oryzae BAK200312, and the preservation number of the Aspergillus oryzae is CGMCC No.19617. The invention provides a rich complex enzyme system. The used aspergillus oryzae strain is obtained by strict domestication, has strong enzyme production capability, high amylase yield and high enzyme activity, and isan aspergillus oryzae strain with efficient amylase production capability.

The invention relates to a fungus fermentation culture medium and a fungus fermentation culture technology, and especially relates to a culture medium and a method for high-yield production of amylase by ascosphaera apis fermentation. The culture medium comprises maltose, yeast extract powder, Ca<2+> and vitamin E. The method comprises that the culture medium is inoculated with strain fermentation liquor, 50ml of the culture medium loaded in a triangular flask having the capacity of 250ml is used, and an inoculation amount ratio is 5%; and the strain fermentation liquor is cultured at a temperature of 25 DEG C at a rotation rate of 140r / min. The method comprises strain activation, strain fermentation liquor culture and fermentation optimization. The culture medium and the method can greatly improve an amylase yield.

The invention discloses a preparation method of a kidney bean extract with no agglutination activity and high alpha-amylase inhibition activity. The method comprises the following steps: treating a kidney bean water solution for 0.5-2 hour by using protease under the conditions of pH2-4 and the temperature of 30-0 DEG C, regulating the pH value, and taking the supernatant; and treating the supernatant to obtain the powdery kidney bean alpha-amylase inhibitor extract. The invention also discloses a high alpha-amylase inhibition activity kidney bean extract without agglutination activity. The invention also discloses application of the acidic hydrolase in extraction of the high alpha-amylase inhibition activity kidney bean extract without agglutination activity. The method can remove agglutinin activity while keeping alpha amylase inhibition activity, has certain in-vitro antioxidant capacity, is simple to operate, high in economic value and easy for process production, and can meet the auxiliary treatment requirements of diabetic patients while meeting the requirement of common consumers for controlling weight.

The invention discloses high amylase corn starch paste and a preparation method thereof. The method comprises the following steps that (1) high amylase corn starch is added into water to obtain high amylase corn starch water suspension; a sodium hydroxide water solution is added into the high amylase corn starch water suspension to be uniformly stirred to obtain a mixed solution; (2) the mixed solution is stirred; then the pH value of the stirred mixed solution is regulated to be neutral, and the high amylase corn starch paste is obtained; and (3) the high amylase corn starch paste is subjected to high-pressure homogenizing processing. The preparation method provided by the invention is simple and reliable, is convenient to operate and does not pollute environment, and the pasting cost of the high amylase corn starch is greatly saved. The high amylase corn starch paste has good liquidity, high transparency, uniform paste structure and no residual starch granules, and the starch paste viscosity of high amylase corn can be regulated through the selection of homogeneous pressure.