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Preparation of amylase-rich compound enzyme as well as strain and application of compound enzyme

A compound enzyme and amylase technology, applied in the field of compound enzymes, can solve problems such as poor compounding effect, achieve the effects of less waste, saving feed cost, and rich fermentation products

Active Publication Date: 2020-08-18
HANGZHOU BIOCOM BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention proposes the preparation of a compound enzyme rich in amylase and its strain and application, which solves the problem of compounding caused by single ratio of enzyme preparation in the prior art The problem of poor performance

Method used

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  • Preparation of amylase-rich compound enzyme as well as strain and application of compound enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A kind of preparation of the compound enzyme that is rich in amylase, preparation method comprises the following steps:

[0046] 1 slant culture

[0047] Aspergillus oryzae BAK200312 strain was taken, and cultured by streaking on the slant of the sterilized PDA test tube in a sterile environment using aseptic operation, and the slant of the test tube after the streaking was placed in an incubator at 30°C for 5-7 days. The formula of the PDA test tube slant medium used is: glucose 1-2%, potato juice 20-30%, agar powder 1.2-1.8%, sterilized at 121° C. for 20 minutes.

[0048] 2 strains liquid culture

[0049]Take out the strains on the slant of the test tube that have been cultivated in step 1, and transfer all the strains on the slant of the test tube to the secondary liquid medium in a clean bench using aseptic operation, culture in a shaker at 30°C for 2-3 days, and shake The bed speed was 160-200 rpm. The medium used is a semi-synthetic medium (adding known salts o...

Embodiment 2

[0074] A kind of preparation of the compound enzyme that is rich in amylase, preparation method comprises the following steps:

[0075] 1 slant culture

[0076] Aspergillus oryzae BAK200312 strain was taken, and cultured by streaking on the slant of the sterilized test tube in a sterile environment using aseptic operation, and the slant of the test tube after the streaking was placed in an incubator at 30°C for 7-10 days. The test tube slant culture medium used is a high-salt Caspian culture medium, and its medium formula is: 2-3% sucrose, 0.2-0.3% sodium nitrate, 0.05-0.1% dipotassium hydrogen phosphate, 0.05-0.1% magnesium sulfate, chloride Potassium 0.05-0.1%, ferrous sulfate 0.001-0.002%, agar powder 1.2-1.8%. Sterilize at 121°C for 20 minutes.

[0077] 2 strains liquid culture

[0078] Take out the strains on the slant of the test tube that have been cultivated in step 1, and transfer all the strains on the slant of the test tube to the secondary liquid medium in a cle...

Embodiment 3

[0084] A kind of preparation of the compound enzyme that is rich in amylase, preparation method comprises the following steps:

[0085] 1 slant culture

[0086] Aspergillus oryzae BAK200312 strain was taken, and cultured by streaking on the slanted surface of the sterilized test tube in a sterile environment using aseptic operation, and the slanted surface of the test tube was cultured in a 30°C incubator for 5-8 days. The medium used is SDA medium, and its medium formula is: animal tissue pepsin hydrolyzate and tryptone equal mixture 1%, glucose 4%, agar powder 1.2-1.8%, pH 5.4-5.8, sterilized at 121°C 20min.

[0087] 2 strains liquid culture

[0088] Take out the strains on the slant of the test tube that have been cultivated in step 1, and transfer all the strains on the slant of the test tube to the secondary liquid medium in a clean bench using aseptic operation, culture in a shaker at 30°C for 2-3 days, and shake The bed speed was 160-200 rpm. The medium used is a se...

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Abstract

The invention provides preparation of an amylase-rich compound enzyme as well as a strain and application of the compound enzyme. A cultured fermentation product contains amylase, neutral proteases, xylanases, cellulases, glucanases and pectinases. Aspergillus oryzae is named as aspergillus oryzae BAK200312, and the preservation number of the Aspergillus oryzae is CGMCC No.19617. The invention provides a rich complex enzyme system. The used aspergillus oryzae strain is obtained by strict domestication, has strong enzyme production capability, high amylase yield and high enzyme activity, and isan aspergillus oryzae strain with efficient amylase production capability.

Description

technical field [0001] The invention relates to the field of compound enzymes, in particular to the preparation of a compound enzyme rich in amylase, its bacterial strain and its application. Background technique [0002] There are many kinds of feed raw materials, including raw grain, grain, soybean, corn, soybean meal, miscellaneous meal, fish meal, meat and bone meal, whey powder, amino acid, vitamin and many other varieties, which together constitute the source of animal energy and nutrition. Starch is the most abundant nutrient in feed, and it is also the main energy source of livestock and poultry, accounting for about 60% of the apparent metabolizable energy of livestock and poultry diets. Starch is a polymer of glucose, which is stored in energy feeds such as corn, wheat, and sorghum in the form of white solid starch particles. Native starch generally contains two components: amylose and amylopectin. Amylose is an unbranched long-chain molecule composed of α-glucos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N9/08C12N9/16C12N9/18C12N9/24C12N9/26C12N9/30C12N9/34C12N9/42C12N9/48C12N9/62C12N1/14A23K50/30A23K50/60A23K10/12A23K10/30A23K10/37A23K10/35A23K20/26A23K20/163A23K20/20A23K20/189A23K20/147C12R1/69
CPCC12N9/2411C12N9/2405C12N9/2437C12N9/248C12N9/62C12N9/2408C12N9/242C12N9/18C12N9/2428C12N9/2445C12N9/485C12N9/2482C12N9/0065C12N9/0089C12N9/16C12Y302/01003C12Y302/01001C12Y302/01039C12Y301/01011C12Y302/01021C12Y302/01037C12Y301/03001C12Y111/01C12Y115/01001C12Y302/01008C12Y302/0102C12Y302/01004C12Y304/21062C12Y111/01006C12Y304/11009C12Y304/1101C12Y304/16005C12Y304/13A23K50/30A23K50/60A23K10/12A23K10/30A23K10/37A23K10/35A23K20/26A23K20/163A23K20/30A23K20/189A23K20/147C12R2001/69C12N1/145Y02P60/87
Inventor 王云龙吴勃徐永雷王天珍王云祥
Owner HANGZHOU BIOCOM BIOLOGICAL TECH
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