Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-efficiency flavor-enhancing strain and application thereof in improving tobacco quality

A flavor-type and high-efficiency technology, applied in the field of microorganisms, can solve the problems of poor smoke quality, inconspicuous aroma enhancement effect, and long aging time of tobacco leaves, so as to reduce miscellaneous gas and irritation, improve aroma quality and aroma quantity, and shorten Effect of Aging Time

Active Publication Date: 2019-06-25
HUBEI CHINA TOBACCO IND
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of long aging time of tobacco leaves, inconspicuous aroma enhancement effect, poor smoke quality, poor smoking effect, etc., and provides a strain of high-efficiency aroma-enhancing Bacillus, and uses the biochemical treatment characteristics of the microorganism to improve the fermentation and aging of tobacco leaves. The actual effect of improving the sensory quality of tobacco leaves

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-efficiency flavor-enhancing strain and application thereof in improving tobacco quality
  • High-efficiency flavor-enhancing strain and application thereof in improving tobacco quality
  • High-efficiency flavor-enhancing strain and application thereof in improving tobacco quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] Isolation and Culture of Microbes from Flue-cured Tobacco Leaves

[0042] Cut flue-cured tobacco leaves naturally stored in different origins (Yunnan, Sichuan, etc.) under aseptic conditions, weigh about 1g, and place them in 10ml of sterilized phosphate buffer solution. After fully mixing in a shaker, Gradually dilute to the appropriate concentration according to ten-fold series, and take 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Bacterial suspensions of five concentrations were used as the objects for spreading bacteria on plates, and spread them on the medium of each plate, with 3 plates for each gradient, adding 0.2mL of liquid to each plate, spreading with a scraper, and warming at 28-30°C. Cultivate in the box for 1-2 days, pick out the single clone with good growth and full colony, and re-stretch to separate and purify.

[0043] After preliminary screening, 39 strains with good growth were obtained from the tobacco leaves of Yunnan flue-cured tobacco, Sichuan flue-c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a high-efficiency flavor-enhancing strain and application thereof in improving the tobacco quality. The high-efficiency flavor-enhancing strain is Bacillus sp. J3 with the preservation number of CCTCC NO: M 2018707. The strain is first separated from the surface of Zimbabwe flue-cured tobacco leaves and found to have high amylase, catalase and protease activities. The wholegenome sequencing finds that the strain contains multiple sets of oxidative stress systems, high activity amylase expression systems and multiple protease gene information. In addition, the strain also has a polyglutamic acid synthesis gene cluster, and can synthesize polyglutamic acid in a large amount extracellularly. On one hand, the Bacillus sp. can shorten the time of artificially aging of the flue-cured tobacco and improve the aroma of the flue-cured tobacco and smoking quality; on the other hand, the strain can be used as a fertilizer synergist for tobacco planting to improve the planting quality of the tobacco from the tobacco planting.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a high-efficiency aroma-enhancing bacterial strain and its application in improving the quality of tobacco. Background technique [0002] The cultivation of flue-cured tobacco in my country has a history of more than 100 years. Flue-cured tobacco has made important contributions to the development of China's economy and society. With the improvement of the quality of tobacco smoking, a series of changes have taken place in the cultivation and production of flue-cured tobacco. [0003] As we all know, the aging of tobacco leaves is an important process in the tobacco processing process. Because the tobacco leaves harvested in the same year have defects of varying degrees in quality, such as rough aroma, heavy soil, strong irritation, and spicy taste, etc., they must go through the aging process to promote the biochemical reactions of the tobacco leaves, so that the above defects can ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/02A24B3/12C05G3/00C12R1/07
Inventor 董爱君熊国玺戴景程邱东茹肜霖
Owner HUBEI CHINA TOBACCO IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com