132 results about "Ginsenoside Rh2" patented technology

The invention relates to application of a 20(S)-ginsenoside Rh2, namely 20(S)-protopanoxadiol-3-O-beta-D-glucopyranoside compound in the field of pharmacy. The compound can confront the swimming fatigue action of tumor-bearing animals and enhance the oxygen deficit resistance and immunologic function of the tumor-bearing animals. The safety experiment shows that the compound has high safety, does not influence the central nervous system, cardiovascular system and respiratory system of animals, and has no mutagenesis function. The clinical research shows that the compound can effectively reduce the brief fatigue index BFI score of a tumor patient, improve the functional evaluation FACTIT-F score of chronic disease treatment, improve the KPS score, and simultaneously relieve Chinese medicine symptoms so as to improve the fatigue degree of the tumor patient. The compound has high safety and good anti-fatigue function under test dose, and can be used for the treatment of fatigue, in particular cancer related fatigue.

The invention relates to a combination made of 20(R)-ginsenoside taken serving as active constitutent and the application of the combination as medicine or health products. The 20(R)-ginsenoside combination mainly comprises the following weight percentage compositions of the components: 30 percent to 60 percent of 20(R)-ginsenoside Rg3, 10 percent to 40 percent of 20(R)-ginsenoside Rh2 and 10 percent to 30 percent of ginsenoside III, wherein the ginsenoside III is 20(R)-protopanoxadiol-3-O-beta-pyrane xylopyranosyl (1-2) beta-D-pyrane glucosyl (1-2) beta -D-pyrane glucoside. The invention relates to application of the drug combination in preparing anticancer aided drug for preventing and curing leucocyte reduction caused by radiotherapy and chemotherapy and in medicine or health products improving human body immunity.

The invention discloses a production method of rare ginsenoside Rh2 and belongs to the field of enzymatic synthesis of ginsenoside. The production method includes that protopanoxadiol is used as a substrate, glycosyltransferase YjiC or YojK screened from bacillus subtilis is used as a catalyst, and the glycosylation of the C3 hydroxyl of the protopanoxadiol is efficiently catalyzed through in-vitro enzyme reaction to generate the ginsenoside Rh2. The production method is cheap in raw materials, high in ginsenoside Rh2 yield, low in production cost and capable of achieving large-scale production of the precious and rare ginsenoside Rh2.

The invention discloses a complex nano particle of ginsenoside Rh2 albumin and a preparation method thereof. According to the invention, albumin is adopted to wrap ginsenoside Rh2 to prepare a soluble albumin nano particle drug administration system. The nano particle is good in dispersion, and has a sustained release effect, so that more drugs can be accumulated near a tumor tissue, the anti-tumor effect is improved, the toxic and side effects of the drugs on normal tissues can be reduced, and the tolerance of a patient is improved.

The invention discloses two ginsenoside Rh2 aliphatic ester compounds. The compounds have the following structural formula described in the specification. In the formula, R is a C4-C22 fatty acid acyl. The invention also provides the chemical synthesis and enzymatic synthesis methods of the ginsenoside Rh2 aliphatic ester compounds, a pharmaceutical composition which takes the compounds as active ingredients and an application of the compounds and the pharmaceutical composition in preparation of an anticancer drug.

The invention relates to a ginseng and soapberry fermentation extract and its application in cosmetics. The extract is characterized by taking ginseng as the raw material, adding soapberries for lactobacillus fermentation, then conducting extraction with ethanol and other solvents, and purifying the extracting solution which can be applied in cosmetics. The fermented ginseng processed extracting solution and its cosmetics can be used for moisture retention, skin whitening, bacteriostasis, anti-senility, skin protection, and caring skin oxidative damage. As the extraction technology makes fully use of active substances in the ginseng and soapberry fermentation extract, such as rare ginsenoside Rh2, CK and the like, as well as the effective skin care effect of fermented soapberries, etc., the antioxidation and nutrition functions of ginseng and soapberry fermentation extract cosmetics are improved. The invention also provides embodiments of skin care cosmetics, and simultaneously elaborates that, compared with white ginseng, the ginseng and soapberry fermentation processed product of the invention contains different ginsenoside and has strong cosmetic application efficacy.

The invention discloses fusarium proliferatum and the method used to manufacture ginsenoside Rh2. It belongs to fusarium proliferatum ECU2042 CGMCC No.1495. The invention catalyzes and hydrolyzes relative common ginsenoside to manufacture rare saponin, and use the fusarium proliferatum to manufacture ginsenoside Rh2. It cost is relative low. And transformation ratio can reach above 60%. The formed saponin purity is not less than 95%.

The invention discloses a ginsenoside-containing medicine composition. The ginsenoside-containing medicine composition comprises the following active ingredients by weight percent: 20-70 percent of ginsenoside Rg, 10-40 percent of ginsenoside Rh2 and 20-50 percent of notoginsenoside ft1. The invention also provides application of the composition to drugs for preventing and treating cancers, auxiliary anti-cancer drugs and medicines or health products used for strengthening the organic immunity. The ginsenoside-containing medicine composition can effectively inhibit tumor cells and stimulate lymphocyte proliferation, has excellent pharmacological effects, and has better security on the basis of saving resources and taking better drug effects.

The invention provides compound configuration ginsenoside Rh2, which relates to plant extracts - ginsenoside, in particular to compound configuration ginsenoside. The invention also relates to application of ginsenoside with the configuration. The compound configuration ginsenoside mainly comprises 20(S) - ginsenoside Rh2 and 20 (R) - ginsenoside Rh2. The compound configuration ginsenoside is characterized in that the mass ratio of the 20 (R) - ginsenoside Rh2 and the 20(S) - ginsenoside Rh2 is 6:4 to 4:6. The compound configuration ginsenoside Rh2 can be applied to preparation of medicines for anticancer drug synergism and attenuation, medicines for treating cancer, medicines for preventing cancer cell transfer and medicines for enhancing the immunologic effect of the organism. The compound configuration ginsenoside has good effects of cancer resistance attenuation, cancer cell inhibition and cancer cell transfer prevention, and can enhance the immunologic effect of the organism.

The invention relates to the application of panaxoside Rh2, Ck and Rg3 to increase the heart contraction force, wherein the medicine compound comprises active panaxoside Rh2 and / or Ck and / or Rg3 and medicine carrier, to be made into injection, oral agent and film agent, to cure acute and low heart cardiac failure or reduced heart contraction force.

The invention discloses glycosyltransferase which can catalyze protopanaxdiol to be glycosylated to synthesize rare ginsenoside Rh2. Multiple mutants with optimized catalytic efficiency are obtained through transformation of the glycosyltransferase. The yield of the rare ginsenoside Rh2 synthesized by the mutants with an in-vitro enzyme method is obviously higher than that of wild type ginsenoside Rh2. The glycosyltransferase and the mutants thereof can be applied to total synthesis of the rare ginsenoside Rh2 in microorganism bodies.

The present invention relates to new use of ginsenoside Rh2, disclosed is ginsenoside Rh2 new use in anti-fatigue, belonging to medical and health food field.

The invention relates to application of a 20(S)-ginsenoside Rh2, namely 3-O-beta-D-glycopyranoside-dammarane-24-alkenyl-3beta, 12beta, 20(S)-triol compound in the field of pharmacy. The compound can effectively confront reserpine related ptosis or akinesia, L-5-HT related mouse tremble, mouse suspension tail or forced swimming acquired desperation and depression and enhance levodopa behavioral effects under test dose, and has no excitation or inhibition function on central pivot. The safety research shows that the compound has high safety, does not influence the central nervous system, cardiovascular system and respiratory system, and has no mutagenesis function. The compound has high safety and good antidepressant function under the test dose, and the action mechanism of the compound can block the reuptake of NA and DA transmitter so as to increase the concentration correlation of two transmitters of synapse and clearance; and the compound can be used for treating depression.

The invention discloses ginsenoside Rh2 ester lipidosome and a preparation method and application. The ginsenoside Rh2 ester lipidosome comprises, by mass percentage, 0.2-0.7% of ginsenoside Rh2 ester, 5-10% of lecithin and 1-2% of cholesterol. The preparation method comprises the steps of weight the ginsenoside Rh2 ester, the lecithin and the cholesterol according to the proportions; adding absolute ethyl alcohol, and taking a water bath at the temperature of 50-55 DEG C till the materials are dissolved; injecting 0.01-0.02 M of a phosphate buffer solution while stirring is conducted; conducting hydration for 20-25 minutes at the temperature of 45-50 DEG C; conducting rotary evaporation to remove ethyl alcohol; conducting ultrasonic treatment for 25-35 minutes, so that the ginsenoside Rh2 ester lipidosome is obtained; and introducing N<2>, conducting sealing, and placing the ginsenoside Rh2 ester lipidosome into a refrigerator for refrigerated preservation. The invention further relates to application of the ginsenoside Rh2 ester lipidosome to preparation of injection or preparations in acceptable dosage forms. The ginsenoside Rh2 ester lipidosome and the preparation method and application are simple in preparation process and high in safety and further have the beneficial effects of being high in targeting performance, slow release property and bioavailability.

The invention relates to a process for preparing ginsenosides Rh2 and Rh3 by using stems and leaves of pseudoginseng root as raw materials, which comprises the following technical flows: (1), extracting saponin from the leaves of the pseudoginseng root, namely cooking the stems and leaves of the pseudoginseng root with water for extraction, absorbing, eluting and concentrating to obtain a thin extract of the ginsenosides of the pseudoginseng root; (2), preparing diol saponins, namely absorbing, eluting, concentrating, drying and crushing the thin extract of the ginsenosides of the pseudoginseng root to obtain the diol saponins; and (3), acid hydrolysis, namely, performing acid hydrolysis on the diol saponins, absorbing, washing with water, eluting, concentrating and drying to obtain a mixture which contains the ginsenosides Rh2 and Rh3. The process of the invention is simple and feasible, high in purification rate and good in quality, and reaches industry standards.

The invention has provided a micro emulsion compound which contains panaxoside Rh2, the panaxoside Rh2 and micro emulsion system is included in the invention. The mentioned micro emulsion system includes oil phase, surface acting agent and accessory surface acting agent. The compound in the invention can be used in the application and industrial production of liquid capsule, solid capsule, capsulae enterosolubilis or micro emulsion pellets based on solid support. The preparing method of the compound is also included in the invention. After being orally taken, the preparation can form O / W micro emulsion the diameter of which is smaller than 100nm. Since the medicine is dispersed in the micro emulsion, it can increase the releasing amount of the medicine and the stability is also increased; the oral bioavailability is 5-6 times of common preparations.

The invention discloses NMN-containing composition as well as a preparation method and an application thereof, and relates to the technical field of medicine. The NMN-containing composition specifically comprises essential components as follows: NMN, grape seed extract, ginsenoside PPD, ginsenoside Rh2, chondroitin sulfate, sialic acid, s-adenosylmethionine, gamma-aminobutyric acid, protein oligopeptide, soybean lecithin and balsam pear seed extract. By the synergistic effect of various components, the composition has good effect of improving sleep, is non-toxic and harmless to users and has no adverse side effects.

The invention relates to the field of microorganism, in particular to a method for preparing ginsenoside Rh2. The method comprises the following steps: taking activated lactobacillus delbrueckii subsp bulgaricus to inoculate MRS (magnetic resonance spectrum) culture medium, adding total ginsenoside; fermenting for 240-248 hours at 37-39 DEG C; collecting fermentation liquor and saponin-glycosidases for reacting at 240-360 hours at 88-92 DEG C; collecting reaction solution for filtration, absorbing the filtrate by using microporous resin and then eluting the absorbed filtrate to by 70 percent ethanol; extracting with 95 percent ethanol; and then filtering, and drying the filtrate to obtain the ginsenoside Rh2. The preparation method is simple to operate, mild for reaction conditions, utilizes the lactobacillus delbrueckii subsp bulgaricus to conduct biotransformation on the total ginsenoside, and saponin-glycosidases to prepare the ginsenoside Rh2, thus being high in transformation rate, being used for preparing a large amount of ginsenoside Rh2, and further realizing the industrialization of the ginsenoside Rh2.

The invention relates to traditional Chinese medicine extract and preparation thereof, in particular to ginsenoside Rh2 extract and preparation thereof. The preparation method comprises the following steps of: 1, extracting a medicinal material which contains ginsenoside components by using water, performing column chromatography on extract liquid through a macroporous adsorption resin column, eluting by using ethanol, collecting eluent, and concentrating the eluent until the eluent is dried so as to obtain total saponins; 2, dissolving the total saponins obtained in the step 1 in acid solution for reaction, adjusting pH to be neutral after the reaction is completed, and collecting precipitate; and 3, dissolving the precipitate in the ethanol, mixing into reverse phase silica gel, performing reverse phase silica gel column chromatography, eluting by using acetonitrile and water mixed liquid, collecting a fraction which is rich in the ginsenoside Rh2, and concentrating the fraction to obtain the ginsenoside Rh2 extract. The prepared ginsenoside Rh2 extract has higher treatment effect and higher purity than that prepared by the prior art.

The invention discloses application of ginsenoside Rh2 and particularly verifies that ginsenoside can be applied to preparation of inhibitors for inhibiting Treg cells or preparation of compositions for preventing and / or treating tumors with Treg cells increased. The ginsenoside Rh2 is capable of improving balance of T cells in human bodies by inhibition of the Treg cells, autoimmunity can be further enhanced, and accordingly an anti-tumor effect or an immunodeficiency disease improvement effect can be achieved.

The invention relates to a preparation using Quezui tea as a raw material and the application thereof. Tests prove that the effect of Quezui tea on improving the immunity function of the mouse of immunity inhabitation is better than that of the same dosage of fermented crordyceps mycelium and basically equal to 10mg.kg<-1> ginsenoside Rg3+10mg.kg<-1>ginsenoside Rh2, thus the preparation can be used for treating the diseases of poor immunity, and the like or treating senile degeneration, such as eye-ground yellow-macular pathological change and senile dementia. During the process of treating the diseases, Quezui tea or the extract thereof is used as an active ingredient to be individually used or cooperatively used with other medicines to be prepared into the medicines of different preparation forms of clinical utilization.

The invention provides a panax notoginseng saponin composition and a preparation method and application thereof.The composition is prepared from, by weight, 30-50% of ginsenoside Rg1, 25-40% of ginsenoside Rb1, 7-16% of notoginsenoside R1, 2.7-8% of ginsenoside Re, 0.5-7.0% of ginsenoside Rd, 0.5-2% of ginsenoside Rf, 0.3-2.0% of ginsenoside Rh2, 1-3% of ginsenoside Rc, 0.3-2.5% of ginsenoside Rb3 and 0.5-2.5% of ginsenoside Rg3, wherein ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 account for 70-95% of the total weight of the active components, and the sum of the contents of ginsenoside Rb1 and notoginsenoside R1 is not smaller than 8 times of the content of ginsenoside Rd.Compared with commercially available Xueshuantong injections and Xueshuantong preparations, the panax notoginseng saponin composition has a more obvious arterial and venous thrombosis resistant effect, low toxicity and high safety.

The invention provides a pharmaceutical composition. The pharmaceutical composition comprises ginseng extract, and an anti-tumor pharmaceutical component 5-fluorouracil, wherein preferably the mass ratio of the ginseng extract to the 5-fluorouracil is 1:1, and the ginseng extract comprises ginsenoside Rb1, ginsenoside Re, ginsenoside Rd, ginsenoside Rb3, ginsenoside Rg3, ginsenoside Rh2, ginsenoside Rg1. The pharmaceutical composition is used to enhance the curative effect of antitumor components and reduce the treatment side-effect caused by the antitumor components. The pharmaceutical composition is especially designed for treating stomach cancer diseases.

The invention relates to a composite microsphere for co-loading adriamycin nanoparticles and ginsenoside rh2 and a preparation method thereof, wherein the preparation method comprises the following steps: (1) preparing adriamycin hydrochloride and a nanoparticle carrier material into doxorubicin polyelectrolyte nanoparticles; (2) dispersing the adriamycin polyelectrolyte nanoparticles in water toform a nano-particle suspension, and dispersing the nano-particle suspension in an organic solution containingginsenoside rh2 and a high-molecular polymer to prepare S / W1 / O colostrum; (3) adding the S / W1 / O colostrum into an aqueous solution containing an emulsifier to prepare S / W1 / O / W2 complex milk; (4) solidifying the S / W1 / O / W2 complex emulsion into microspheres, collecting the microspheres, washing and drying. The microsphere prepared by the method can achieve the sequential release, slow release of doxorubicin hydrochloride and ginsenoside rh2,and has the advantages of low burst release rate and high encapsulation rate.

The present invention is the preparation process of 20(R)-ginsenoside Rh2 with panax leaf and stem total saponin or protopanoxadiol saponin as initial material. The preparation process includes dissolving the material in the water solution or alcohol solution of organic acid or inorganic acid for acid hydrolysis and collecting the reaction product. The product may be silica gel chromatographic column separated and re-crystallized to purify. The acid hydrolysis is completed at 30-100 deg.c and 0.05-1 MPa, and the organic acid and the inorganic acid include formic acid, acetic acid, hydrochloric acid, sulfuric acid and nitric acid. In addition, the prepare process of medicine composition with 20(R)-ginsenoside Rh2 is also disclosed. The present invention has reasonable technological process and high yield.

The invention provides application of ginsenoside Rh2 in preparation of medicines for preventing and treating inflammatory bowel diseases. According to the application in the invention, the ginsenoside Rh2 is capable of increasing the content of beneficial microorganisms in the intestinal tract and reducing the content of harmful microbes in the intestinal tract by regulating microbial equilibriumin the intestinal tract, and intestinal flora disturbance is avoided, so that the content of inflammatory factors such as TNF-alpha, IL-6 and IL-1beta in the colon is reduced. Therefore, the inflammatory bowel diseases can be effectively treated.

The invention relates to a preparation method of violet gold black ginseng. The preparation method comprises carrying out wall breaking treatment on cleaned ginseng, carrying out drying, and carrying out fermentation treatment under the conditions of control of a temperature and humidity. Through the preparation method, in violet gold black ginseng preparation, high-content secondary ginsenoside components Rg3 and Rh2 are formed. A detection result shows that compared with black ginseng obtained by the traditional method, the violet gold black ginseng has ginsenoside Rg3 content improved by 25-26% and ginsenoside Rh2 content improved by 3 times, and obviously improves health care effects and medicinal value. The preparation method is not influenced by natural conditions, has large scale batch production feasibility, realizes violet gold black ginseng product quality data control and prevents difference of each batch of products. The violet gold black ginseng has a sweet-waxy taste.

The invention relates to the technical field of medicine and discloses a preparing method for ginsenoside Rh2. The preparing method comprises the steps of strain culture, raw material selecting, biological fermentation and conversion, filter pressing, sterilization and drying, and thus a product is obtained. Ginsenoside Rh2 extract prepared through the preparing method is high in treatment effect and purity, good in absorption and stable in quality; meanwhile, the preparing method is good in separation effect, high in content, simple in process, convenient to implement, low in cost and suitable for industrial production.