47 results about "Free-flow electrophoresis" patented technology

Electrophoretically enhanced methods are disclosed for carrying out binding and other reactions using pulsating and polarity reversing electric fields. The methods are exemplified by E3 ELISAs using free flow electrophoresis in multiwell plates and E3 Westerns using saturated matrices. The E3 methods are much faster than conventional methods and provide superior results. Reagents, buffers, devices and power supplied for carrying out E3 methods are disclosed.

The present invention provides a novel and advantageous method for separating analytes by free flow electrophoresis. The methods are particularly suitable for depleting major constituents such as albumin from samples of biological origin, optionally combined with a further separation of the remaining portion of the sample. The sample portions recovered from the method can be used advantageously in downstream applications such as 1D or 2D-PAGE, HPLC or mass spectrometric analysis. Also provided are buffer systems, kits comprising such buffer systems, and devices for carrying out the free flow electrophoretic separation methods of the present invention.

A device for conducting integrated sequential separation and enrichment of a mixture of sample proteins, comprising means for separation and means for enrichment, whereby free flow electrophoresis—isoelectric focussing (FFE-IEF), is arranged to take place within an etched system of basins and channels is preferably employed in the separation step, multichannel conduits are used to guide the separated protein fractions to the enrichment stage, and a solid phase micro-extraction procedure, in an optionally dockable format, is preferably employed in the enrichment step. The separated fractions are then preferably dispensed onto a MALDI-plate for subsequent matrix assisted laser desorption ionisation (MALDI) analysis.

The present invention provides a novel and advantageous method for separating analytes by free flow electrophoresis. The methods are particularly suitable for depleting major constituents such as albumin from samples of biological origin, optionally combined with a further separation of the remaining portion of the sample. The sample portions recovered from the method can be used advantageously in downstream applications such as 1D or 2D-PAGE, HPLC or mass spectrometric analysis. Also provided are buffer systems, kits comprising such buffer systems, and devices for carrying out the free flow electrophoretic separation methods of the present invention.

The invention relates to the use of MS compatible surfactants in free-flow electrophoretic methods, which allow the separation of analytes with differentiated electrophoretic mobility. The surfactant is preferably a cleavable surfactant, such as PPS.

The invention discloses an air exhausting device of a free-flow electrophoresis separation cavity and an implementation method of the air exhausting device, and belongs to the technical field of biochemical engineering. The air exhausting device comprises an air inflating pump, a hose clamp, a rubber hose, an air-liquid buffering and separation device, a self-balancing recycling device, a separation cavity shell, a separation cavity, a waste liquid recycling bottle and a recycling three-way valve. According to the air exhausting device, the liquid storage function of the air-liquid buffering and separation device is utilized, firstly, the buffering fluid which is over ten times as large as the volume of the separation cavity is stored in the air-liquid buffering and separation device, then the buffering fluid stored in the air-liquid buffering and separation device quickly enters into the separation cavity by virtue of a hand-operated air inflating pump, as the speed of the buffering fluid is high, the separation cavity can be filled with the buffering fluid soon, thus the air in the separation cavity is exhausted completely. The device disclosed by the invention can be used for exhausting the air in the separation cavity by utilizing very simple operations within a short period of time, so that the problem that the former separation cavity is cockamamie and difficult to exhaust air; and the device has the advantages of simple structure, low cost, practicability and effectiveness, and is easy to operate and the like.

The invention relates to the use of MS compatible surfactants in free-flow electrophoretic methods, which allow the separation of analytes with differentiated electrophoretic mobility. The surfactant is preferably a cleavable surfactant, such as PPS.

The present invention provides methods and separation media for separating analytes of interest via free flow electrophoresis (FFE) using volatile buffer systems. The separation media provided herein allow a convenient separation of the analytes by electrophoresis, and offer the additional advantage that the buffer compounds and the solvent can be easily and residue-free removed after the electrophoretic separation step. Furthermore, methods for mass spectrometric analysis of analytes comprising an FFE method and kits for carrying out FFE separations with volatile buffer systems are also provided. Preferably, the volatile buffer system is TRIS acetate.

A buffering diverter for the free flow electrophoresis is disclosed, which is composed of a buffer cavity consisting of liquid storage chamber and air cushion chamber for buffering the pressure-flow speed pulses caused by liquid delivering pump, electrolyte / specimen solution inlet tube, air cushion regulating injector the separation chamber for free flow electrophorsis level meter and horizontal level regulating knob. The tens-hundreds stable liquid streams can be generated by its outlet tube.

The invention relates to a soil compound heavy metal pollution treatment and resource recovery method, comprising the following steps: 1) adding adsorption materials; 2) magnetic separation; 3) desorption of the adsorption materials; 4) one-time adjustment of the desorption liquid; Secondary adjustment of desorption liquid; 6) supergravity centrifugal extraction of heavy metals; 7) free flow electrophoresis; the present invention has the advantages of diverse types of heavy metals, high removal efficiency, and no secondary pollution, and can accurately separate and recycle complex heavy metals , which can be applied to in-situ remediation or ex-situ remediation of heavy metal-contaminated soils.

The present invention provides an isoelectric point standard substance for free flow electrophoresis, the isoelectric point standard substance comprises eight isoelectric point substances, each isoelectric point substance corresponds to a different isoelectric point, each isoelectric point substance has a color capable of being directly observed by naked eyes, the isoelectric point of each isoelectric point substance is between 3.8 and 10.1, the isoelectric point substance is composed of micromolecular substances diluted by ultrapure water, the molecular weight of the isoelectric point substances is 200-600 KDa, and the isoelectric point substances are benzene ring and heterocyclic ring substances. The isoelectric point standard substance meets the system standards of pH 3-10, pH 7-10 and pH 5-8, the pH can be positioned in the isomer separation and preparation process, the isomer positioning is greatly improved, and the isomer preparation time is shortened.

The invention relates to the field of biological equipment manufacturing engineering. A protein and nucleic acid separation device includes: a macromolecule sample storage area, a buffer storage area, a free-flow electrophoresis chip, a protein collection area, a nucleic acid collection area, and a power supply; the free-flow electrophoresis chip An electrophoresis channel is arranged inside, and electrodes are arranged on both sides of the electrophoresis channel, and the positive and negative poles of the power supply are respectively connected to the electrodes on both sides; the inflow end of the electrophoresis channel is provided with a sample inlet and a buffer inlet, and the electrophoresis channel A protein outlet and a nucleic acid outlet are respectively set at the outflow end of the outlet near the electrodes on both sides; the sample inlet is connected to the macromolecule sample storage area, and the buffer inlet is connected to the buffer storage area; the protein outlet is connected to the A protein collection area, the nucleic acid outlet is connected to the nucleic acid collection area. The protein-nucleic acid separation device of the present invention has a compact and simple structure, is easy to operate, and can realize efficient and rapid separation of protein and nucleic acid.

In a non-carrier electrophoresis process to separate a sample substance into its constituent parts, the sample is first subjected to coarse fractionation followed by a second-stage fine fractionation. In the first stage is conducted at high linear flow speed over a short migration distance. A smaller number of fractionating points is used in the first stage than in the second stage. The process stages may be conducted in parallel, in series, or in a combination of parallel and series. An independent claim is also included for an electrophoresis apparatus with a separation chamber, electrodes, sample and fraction sections. The separation chamber is sub-divided into smaller chambers with electrodes each serving two fraction chambers,.