47 results about "Free-flow electrophoresis" patented technology

Electrophoretically enhanced methods are disclosed for carrying out binding and other reactions using pulsating and polarity reversing electric fields. The methods are exemplified by E3 ELISAs using free flow electrophoresis in multiwell plates and E3 Westerns using saturated matrices. The E3 methods are much faster than conventional methods and provide superior results. Reagents, buffers, devices and power supplied for carrying out E3 methods are disclosed.

The present invention provides a novel and advantageous method for separating analytes by free flow electrophoresis. The methods are particularly suitable for depleting major constituents such as albumin from samples of biological origin, optionally combined with a further separation of the remaining portion of the sample. The sample portions recovered from the method can be used advantageously in downstream applications such as 1D or 2D-PAGE, HPLC or mass spectrometric analysis. Also provided are buffer systems, kits comprising such buffer systems, and devices for carrying out the free flow electrophoretic separation methods of the present invention.

A device for conducting integrated sequential separation and enrichment of a mixture of sample proteins, comprising means for separation and means for enrichment, whereby free flow electrophoresis—isoelectric focussing (FFE-IEF), is arranged to take place within an etched system of basins and channels is preferably employed in the separation step, multichannel conduits are used to guide the separated protein fractions to the enrichment stage, and a solid phase micro-extraction procedure, in an optionally dockable format, is preferably employed in the enrichment step. The separated fractions are then preferably dispensed onto a MALDI-plate for subsequent matrix assisted laser desorption ionisation (MALDI) analysis.

The invention relates to an Off-Adhesive free flow electrophoresis coupling chip and a making method thereof, belonging to the technical field of microfluidic chip manufacture. The chip comprises a chip substrate, an electrode and a separating chamber, wherein an immobilized pH gradient adhesive tape area and hard substrates for fixing and embedding of immobilized pH gradient adhesive are arranged at the tail end of the separating chamber. The method comprises the following steps: arranging a rectangular area for embedding of the immobilized pH gradient adhesive tape at the tail end of the separating chamber of the other substrate, then aligning the chip substrate with another substrate, carrying out sealing connection on the two substrates and the electrode according to a combined sealing method of the free flow electrophoresis chip to form a free flow electrophoresis chip, arranging the chosen immobilized pH gradient adhesive tape at the rectangular slotting area of the chip, and pressing and fixing the immobilized pH gradient adhesive tape by using the other hard substrate for using. The chip not only solves the defects of the free flow electrophoresis chip and Off-Adhesive electrophoresis technologies, but also inherits the advantages of the two technologies; and the chip has simple manufacturing method, and the immobilized pH gradient adhesive tape is replaceable.

The present invention provides methods and separation media for separating analytes of interest via free flow electrophoresis (FFE) using volatile buffer systems. The separation media provided herein allow a convenient separation of the analytes by electrophoresis, and offer the additional advantage that the buffer compounds and the solvent can be easily and residue-free removed after the electrophoretic separation step. Furthermore, methods for mass spectrometric analysis of analytes comprising an FFE method and kits for carrying out FFE separations with volatile buffer systems are also provided. Preferably, the volatile buffer system is TRIS acetate.

The invention relates to a soil compound heavy metal pollution regulation and recycling method. The method includes the following steps that (1) an adsorbing material is put; (2) magnetic separation is carried out; (3) adsorbing material desorbing is carried out; (4) primary adjustment of desorbing liquid is carried out; (5) sheet frame filtering and secondary adjustment of the desorbing liquid are carried out; (6) supergravity heavy metal centrifugal extraction is carried out; and (7) free flow electrophoresis is carried out. The soil compound heavy metal pollution regulation and recycling method has the beneficial effects that heavy metal treatment type diversification is achieved, the removal rate is high, and secondary pollution is avoided; compound heavy metal can be accurately separated and recovered; and the method can be applied to in-situ remediation or out-situ remediation of heavy metal polluted soil.

A method for separating particles in free-flow electrophoresis FFE includes conveying a separation medium using a dosage pump into a separation chamber; releasing the separation medium via outlets; applying an electrical field in the separation medium; coupling a particle to be separated with selectively defined charge labels for generating charge-modified particles which due to their selectively modified net surface charge display a different migratory behavior in FFE than non-charge-modified particles; adding a mixture of particles to be separated to the separation medium in the separation chamber by way of sample injection points; removing the particles separated using the separation medium by way of fractionation points; and collecting the fractions with the separated particles. Focusing buffers are created using a buffer medium between the separation medium and at least one electrode, whereby this buffer medium is much more electrically conductive than the separation medium and is fed into these focusing buffers.

The invention discloses a free-flow electrophoresis separation chamber device used in the technical field of biochemical engineering. The device comprises an organic glass flat plate, a ceramic flat plate, an ionic membrane, an electrophoresis chamber, an electrode chamber, a fastening sealed structure and an automatic semiconductor refrigeration and heat dissipation device, wherein the ionic membrane is located between an upper organic glass flat plate and a lower ceramic flat plate, and the middle and two sides of the ionic membrane are hollowed, and the ionic membrane, the upper organic glass flat plate and the lower ceramic flat plate form the electrophoresis chamber and the electrode chamber; a plurality of liquid inlet holes are uniformly formed on one end of the upper organic glass flat plate, and double amount of liquid outlet holes are uniformly formed on the other end of the upper organic glass flat plate, thus forming a swimming lane. The device disclosed by the invention can effectively solve the problem that a separation chamber is easy to leak, can effectively eliminate joule heat in the separation chamber, can improve the stability of flowing shape of the fluid in the separation chamber, and is easy to assemble; the device can be widely used for routine free-flow electrophoresis separation of multiple types of samples such as small-molecule substances, protein, organelle and virus, and meanwhile, the device can be applied to purification of samples.

A separation chamber device used for preparative free-flow electrophoresis (FFE) comprises a first synthetic glass flat plate, a second synthetic glass flat plate, an electrophoresis chamber and an electrode chamber; the electrophoresis chamber and the electrode chamber, which are formed by a separation hole and an electrode hole of an ion-exchange membrane, and a plastic film respectively, are clamped between the first and the second synthetic glass flat plates; a plastic screw passes through an upper screw hole on the first synthetic glass flat plate, a lower screw hole on the second synthetic glass flat plate and a film screw hole of the plastic film to be fixed; and an electrode buffer solution inlet and an electrode buffer solution outlet are formed on the first synthetic glass flat plate and at the positions corresponding to two ends of the electrode hole of the ion-exchange membrane and are used for circulating electrode solution. The invention can solve the problems of leak tightness and stability of the traditional FFE, provides a high-stability and high-uniformity hydrodynamics environment, is extensively applied to conventional FFE separation of various samples such as nucleic acid / protein / polypeptide / virus, and can be applied to various sample purifications carried out in FFE devices.

A self-balanced separated liquid collector for free flow electrophoresis (FFE) has multiple specimen bottles which are installed on a rigid frame and communicated to the FFE separation chamber via respective inlet tube. The level meter and level regulating knob are used to regulate their level position. It can be used for conventional FFE separation of nucleic acid, protein, polypeptide, cell, virus, etc.

A free-flow electrophoresis (FFE) apparatus and separation medium for separating particles therefore, includes a separation chamber with a dosage pump for conveying medium, electrodes for applying an electric field in the medium and sample injection points for adding a mixture of particles to be separated and fractionation points for removing the particles. Definable charge labels couple with the particles to form charge-modified particles which display with a different migratory behavior in FFE The apparatus includes focusing buffers between the separation medium and at least one electrode which has much higher electrical conductivity than the separation medium.

The invention relates to a microfluidic free flow paper chromatographic array spray mass spectrometry device. The device comprises a microfluidic control device, a microfluidic separation device, an array spray component and a mass spectrum component, wherein the microfluidic control device is used for controlling sample loading and flow of a mobile phase or a buffer solution. The microfluidic separation device is composed of a microfluidic support plate, a microfluidic cover plate and a paper stationary phase, wherein a metal electrode used for providing a separated electric field is plated on the support plate; a channel used for sample loading and mobile phase transport is reserved in the front end of the cover plate; a separating cavity for separating and filling the paper stationary phase is reserved in the middle of the cover plate; a fraction outlet is formed in the tail end of the separating cavity; the separating cavity is filled with the paper stationary phase and the paper stationary phase is used for separating the target object; the fraction outlet is connected with an array spray device; and mass spectrometry is performed after spray of the array spray device. According to the device disclosed by the invention, the advantages of free flow electrophoresis, chromatography and mass spectrometry are combined, the separation detection efficiency of the traditional freeflow electrophoresis can be very effectively improved, separation is performed in a transverse direction by utilizing the electric field effect, and the separating effect is further achieved in the longitudinal direction according to a chromatographic mechanism.

The invention relates to a device for separating precious metals from a pregnant solution based on a free flow electrophoresis technology, and a use thereof. The device comprises an upper plate, a lower plate, an electrophoresis chamber and an electrode chamber. Plastic films which are located in the upper plate and the lower plate, are hollowed and are subjected to laser engraving molding form the electrophoresis chamber and the electrode chamber. An end of the lower plate is provided with a sample inlet hole and the sample inlet hole is located at an upper side of the trapezoidal structure. An end of the upper plate is provided with multiple sample outlet holes and the multiple sample outlet holes are located at a lower side of the trapezoidal structure. Inert electrodes are installed in the electrode chamber and are used for production of electric independent fields having the difference. The device creatively utilizes continuous separation advantage of free flow electrophoresis in industrial production, widens a free flow electrophoresis application scope, and improves industrial pregnant solution separation efficiency. Through free flow electrophoresis-based preliminary grading, the pregnant solution concentration is improved by 60%. The device provided by the invention is a novel free flow electrophoresis device, has a low cost and a simple structure, and can be assembled easily.

The invention discloses an air exhausting device of a free-flow electrophoresis separation cavity and an implementation method of the air exhausting device, and belongs to the technical field of biochemical engineering. The air exhausting device comprises an air inflating pump, a hose clamp, a rubber hose, an air-liquid buffering and separation device, a self-balancing recycling device, a separation cavity shell, a separation cavity, a waste liquid recycling bottle and a recycling three-way valve. According to the air exhausting device, the liquid storage function of the air-liquid buffering and separation device is utilized, firstly, the buffering fluid which is over ten times as large as the volume of the separation cavity is stored in the air-liquid buffering and separation device, then the buffering fluid stored in the air-liquid buffering and separation device quickly enters into the separation cavity by virtue of a hand-operated air inflating pump, as the speed of the buffering fluid is high, the separation cavity can be filled with the buffering fluid soon, thus the air in the separation cavity is exhausted completely. The device disclosed by the invention can be used for exhausting the air in the separation cavity by utilizing very simple operations within a short period of time, so that the problem that the former separation cavity is cockamamie and difficult to exhaust air; and the device has the advantages of simple structure, low cost, practicability and effectiveness, and is easy to operate and the like.

The invention relates to a phycocyanin separating and enriching device based on free flow electrophoresis. The phycocyanin separating and enriching device comprises a free flow electrophoresis main body separating device, a collecting device, an infusion pump and a pipeline. The free flow electrophoresis main body separating device is different from a common free flow electrophoresis device, multiple injection ports are formed, but a buffer solution inlet is not formed, and the upper end is arc-shaped, so that a stagnation phenomenon is weakened. By utilizing the characteristics that free flow electrophoresis can realize continuous separation and different electric matters have different migration rates in an electric field, phycocyanin crude extract and even protoplasm of cyanobacteria cells after disruption can directly enter the device to be enriched and separated, and an effective way is provided for rapid and efficient large-scale phycocyanin preparation.

The invention relates to the field of biological equipment manufacturing engineering. A protein and nucleic acid separation device comprises a macromolecular sample storage area, a buffer solution storage area, a free flow electrophoresis chip, a protein collection area, a nucleic acid collecting area and a power supply, wherein an electrophoresis channel is formed inside the free flow electrophoresis chip; electrodes are arranged on the two sides of the electrophoresis channel; positive and negative electrodes of the power supply are respectively connected to the electrodes on the two sides; a sample inlet and a buffer solution inlet are formed in the inflow end of the electrophoresis channel; a protein outlet and a nucleic acid outlet are respectively formed in positions, close to the electrodes on the two sides, at the outflow end of the electrophoresis channel; the sample inlet is connected with the macromolecular sample storage area; the buffer solution inlet is connected with the buffer solution storage area; the protein outlet is connected with the protein collecting area; the nucleic acid outlet is connected with the nucleic acid collecting area. The protein and nucleic acid separation device provided by the invention is compact and simple in structure, and easy to operate, and can realize efficient and fast separation of protein from nucleic acid.

A free flow electrophoresis method for separating sample substances into their analytes. This method comprises multiple steps: a first step for the crude fractionation of the sample substance and at least one second step in which the crudely fractionated sample substance is finely fractionated. These steps can be performed in a parallel simultaneous method, in a serial method or in a combination of these two methods.

A method for purification and separation of mixed elements, comprising at least a first free flow electrophoresis separation chamber, wherein a solution of the mixed elements is passed through the first separation chamber, an electric field submitted perpendicular to the solution flow and separating mobile ions of the solution based on electrophoretic mobility. A continuous method comprises selecting a complexing ligand, and controlling the temperature and pH of the solution. Also, a system or method for separating components of a multi-component concentrate comprising directing solution to at least a first and second channel each receiving part of the solution; each channel comprising a concentration section comprising a first transverse electric field across the channel, a fractionation section comprising a second electric field in a direction opposite the first electric field thereby distributing ions of the solution across the channel, and a flow splitter at an output of the fractionation section that divides the flow of each channel into subflows concentrated in heavier elements and concentrated in lighter elements.

The invention discloses a free flow electrophoresis chip of an adhesive tape and a processing method thereof, and relates to a simple micro-fluidic chip and a processing method thereof. The preparation method comprises the steps of: 1, cleaning and processing upper and lower substrates, pasting the designed chip structure patterns on the upper and lower substrates as alignment marks; 2, punching a liquid inlet hole on the upper substrate, and pasting a single sticky tape by aiming a separation chamber mark; 3, pasting a double sticky tape to the lower substrate, calibrating the pattern mark by a nicking tool under a microscope, removing excessive adhesive tape, and then pasting the single sticky tape and a conductive adhesive tape by aiming the mark; 4, removing a surface film of the double sticky tape on the lower substrate, and cementing the upper substrate and the lower substrate by alignment. The free flow electrophoresis chip of the adhesive tape does not need to use any expensive special device under a common process environment, and is simple in process, and low in construction cost, and can meet the accuracy of a certain structure.

The invention relates to a device for continuously removing metal ions from a feed solution and a use method. According to the device, a free-flow electrophoresis technology and ion-exchange chromatography are combined, so that the efficient and continuous removal of the metal ions is achieved. The device comprises parts such as a separation chamber, an adsorption chamber and an electrode system. The device and the use method have the advantages that the device is low in manufacturing cost, simple in structure, easy to assemble and convenient to operate; the separation chamber is large in size, so that the feed solution treatment capacity is high; the metal ions in the feed solution can be continuously removed; the feed solution does not directly flow through a resin cavity, so that secondary pollution cannot be caused.

The present invention provides methods and separation media for separating analytes of interest via free flow electrophoresis (FFE) using volatile buffer systems. The separation media provided herein allow a convenient separation of the analytes by electrophoresis, and offer the additional advantage that the buffer compounds and the solvent can be easily and residue-free removed after the electrophoretic separation step. Furthermore, methods for mass spectrometric analysis of analytes comprising an FFE method and kits for carrying out FFE separations with volatile buffer systems are also provided. Preferably, the volatile buffer system is TRIS acetate.

The invention relates to a soil compound heavy metal pollution treatment and resource recovery method, comprising the following steps: 1) adding adsorption materials; 2) magnetic separation; 3) desorption of the adsorption materials; 4) one-time adjustment of the desorption liquid; Secondary adjustment of desorption liquid; 6) supergravity centrifugal extraction of heavy metals; 7) free flow electrophoresis; the present invention has the advantages of diverse types of heavy metals, high removal efficiency, and no secondary pollution, and can accurately separate and recycle complex heavy metals , which can be applied to in-situ remediation or ex-situ remediation of heavy metal-contaminated soils.

The present invention provides an isoelectric point standard substance for free flow electrophoresis, the isoelectric point standard substance comprises eight isoelectric point substances, each isoelectric point substance corresponds to a different isoelectric point, each isoelectric point substance has a color capable of being directly observed by naked eyes, the isoelectric point of each isoelectric point substance is between 3.8 and 10.1, the isoelectric point substance is composed of micromolecular substances diluted by ultrapure water, the molecular weight of the isoelectric point substances is 200-600 KDa, and the isoelectric point substances are benzene ring and heterocyclic ring substances. The isoelectric point standard substance meets the system standards of pH 3-10, pH 7-10 and pH 5-8, the pH can be positioned in the isomer separation and preparation process, the isomer positioning is greatly improved, and the isomer preparation time is shortened.

The present invention relates to a Micro-Free Flow Electrophoresis chip for analyzing or separating a sample including a pile (1) comprising at least two plates (2A, 2B), a sheet (3) uniformly disposed between the two plates (2A, 2B), clamping means, each sheet (3) comprising at least two inlets (11) for entry and at least one outlet (12) for exit of a first fluid electrode and a second fluid electrode, the first and second fluid electrodes applying an electric field to a separation chamber (31).

In a non-carrier electrophoresis process to separate a sample substance into its constituent parts, the sample is first subjected to coarse fractionation followed by a second-stage fine fractionation. In the first stage is conducted at high linear flow speed over a short migration distance. A smaller number of fractionating points is used in the first stage than in the second stage. The process stages may be conducted in parallel, in series, or in a combination of parallel and series. An independent claim is also included for an electrophoresis apparatus with a separation chamber, electrodes, sample and fraction sections. The separation chamber is sub-divided into smaller chambers with electrodes each serving two fraction chambers,.