Protein and nucleic acid separation device and method

Abstract

The invention relates to the field of biological equipment manufacturing engineering. A protein and nucleic acid separation device comprises a macromolecular sample storage area, a buffer solution storage area, a free flow electrophoresis chip, a protein collection area, a nucleic acid collecting area and a power supply, wherein an electrophoresis channel is formed inside the free flow electrophoresis chip; electrodes are arranged on the two sides of the electrophoresis channel; positive and negative electrodes of the power supply are respectively connected to the electrodes on the two sides; a sample inlet and a buffer solution inlet are formed in the inflow end of the electrophoresis channel; a protein outlet and a nucleic acid outlet are respectively formed in positions, close to the electrodes on the two sides, at the outflow end of the electrophoresis channel; the sample inlet is connected with the macromolecular sample storage area; the buffer solution inlet is connected with the buffer solution storage area; the protein outlet is connected with the protein collecting area; the nucleic acid outlet is connected with the nucleic acid collecting area. The protein and nucleic acid separation device provided by the invention is compact and simple in structure, and easy to operate, and can realize efficient and fast separation of protein from nucleic acid.

Description

technical field [0001] The invention relates to the field of biological equipment manufacturing engineering, in particular to a protein and nucleic acid separation device and method. Background technique [0002] In the current field of life science research, two kinds of biomacromolecules, protein and nucleic acid, are important research objects. Protein is responsible for various physiological functions, maintains the metabolic activities of organisms as a whole, and is the direct expresser of biological traits, while nucleic acid is the main substance that reveals the inheritance and variation of life. In the analysis and research of biological samples, the samples often contain a large amount of protein and nucleic acid, which requires separation and purification of the protein or nucleic acid. There are many separation and purification methods for biological macromolecules, such as traditional dialysis, ultrafiltration, coagulation, etc. Gel filtration, organic precipi...

AI Technical Summary

Problems solved by technology

However, these separation operations often affect or even destroy the activity of another biomacromolecule when separating one biomacromolecule.

Moreover, these methods usually require relatively complicated manual operations or the use of multiple instruments and equipment, and these operations also have many shortcomings in terms of time and separation efficiency.

[0003] Therefore, in the field of separation and purification of biomacromolecules, there is still a lack of a miniaturized and automated separation device for the whole protein and nucleic acid of cells.

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