Microfluidic free flow paper chromatographic array spray mass spectrometry device

Abstract

The invention relates to a microfluidic free flow paper chromatographic array spray mass spectrometry device. The device comprises a microfluidic control device, a microfluidic separation device, an array spray component and a mass spectrum component, wherein the microfluidic control device is used for controlling sample loading and flow of a mobile phase or a buffer solution. The microfluidic separation device is composed of a microfluidic support plate, a microfluidic cover plate and a paper stationary phase, wherein a metal electrode used for providing a separated electric field is plated on the support plate; a channel used for sample loading and mobile phase transport is reserved in the front end of the cover plate; a separating cavity for separating and filling the paper stationary phase is reserved in the middle of the cover plate; a fraction outlet is formed in the tail end of the separating cavity; the separating cavity is filled with the paper stationary phase and the paper stationary phase is used for separating the target object; the fraction outlet is connected with an array spray device; and mass spectrometry is performed after spray of the array spray device. According to the device disclosed by the invention, the advantages of free flow electrophoresis, chromatography and mass spectrometry are combined, the separation detection efficiency of the traditional freeflow electrophoresis can be very effectively improved, separation is performed in a transverse direction by utilizing the electric field effect, and the separating effect is further achieved in the longitudinal direction according to a chromatographic mechanism.

Description

Technical field [0001] The invention relates to the field of chromatography and mass spectrometry, in particular to a microfluidic free-flow paper chromatography array spray mass spectrometry device. Background technique [0002] In the past 30 years, two-dimensional separation and analysis technology has developed rapidly and has been widely used in the separation and analysis of complex biological, chemical, medical, and environmental samples. The biggest advantage of this separation analysis method is that it has a very high peak capacity. So far, there have been many two-dimensional separation methods, including capillary electrophoresis-high performance liquid chromatography, capillary electrophoresis-capillary electrophoresis, etc. Good results. Based on the advantages of high integration, high throughput, and low consumption, microfluidic chip technology has become one of the important means of life science research. It uses the chip as the basic platform to establish a ...

AI Technical Summary

Problems solved by technology

However, the current glycoprotein separation methods are mainly liquid chromatography, gel chromatography, solid phase extraction and other methods. Among these methods, some have problems such as low separation efficiency and some have time-consuming problems.

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